Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Vasco Fachada; Paavo Rahkila; Nuno Fachada; Tuomas Turpeinen; Urho  Kujala; Heikki  Kainulainen

doi:10.1016/j.acthis.2022.151869

Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Vasco Fachada (Corresponding Author), Paavo Rahkila, Nuno Fachada, Tuomas Turpeinen, Urho Kujala, Heikki Kainulainen

BA5401 Fiber web processes

Research output: Contribution to journal › Article › Scientific › peer-review

3 Citations (Scopus)

Abstract

Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.

Original language	English
Article number	151869
Journal	Acta Histochemica
Volume	124
Issue number	3
DOIs	https://doi.org/10.1016/j.acthis.2022.151869
Publication status	Published - Apr 2022
MoE publication type	A1 Journal article-refereed

Keywords

Lipid droplets
PLIN5
Type II diabetes
Skeletal muscle
Insulin resistance
Fiber type

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.acthis.2022.151869Licence: CC BY

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title = "Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes",

abstract = "Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.",

keywords = "Lipid droplets, PLIN5, Type II diabetes, Skeletal muscle, Insulin resistance, Fiber type",

author = "Vasco Fachada and Paavo Rahkila and Nuno Fachada and Tuomas Turpeinen and Urho Kujala and Heikki Kainulainen",

note = "Funding Information: This study was supported by the Academy of Finland (grant 132987 and 298875) and Funda??o para a Ci?ncia e a Tecnologia (FCT, Portugal) under grant UIDB/04111/2020 (COPELABS). The author Vasco Fachada acknowledges his FCT grant SFRH/BD/68308/2010. Funding Information: This study was supported by the Academy of Finland (grant 132987 and 298875 ) and Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT, Portugal) under grant UIDB/04111/2020 (COPELABS). The author Vasco Fachada acknowledges his FCT grant SFRH/BD/68308/2010. Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

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doi = "10.1016/j.acthis.2022.151869",

language = "English",

volume = "124",

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AU - Fachada, Vasco

AU - Rahkila, Paavo

AU - Fachada, Nuno

AU - Turpeinen, Tuomas

AU - Kujala, Urho

AU - Kainulainen, Heikki

N1 - Funding Information: This study was supported by the Academy of Finland (grant 132987 and 298875) and Funda??o para a Ci?ncia e a Tecnologia (FCT, Portugal) under grant UIDB/04111/2020 (COPELABS). The author Vasco Fachada acknowledges his FCT grant SFRH/BD/68308/2010. Funding Information: This study was supported by the Academy of Finland (grant 132987 and 298875 ) and Fundação para a Ciência e a Tecnologia (FCT, Portugal) under grant UIDB/04111/2020 (COPELABS). The author Vasco Fachada acknowledges his FCT grant SFRH/BD/68308/2010. Publisher Copyright: © 2022 The Authors

PY - 2022/4

Y1 - 2022/4

N2 - Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.

AB - Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.

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Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Abstract

Keywords

UN SDGs

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