Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Vasco Fachada (Corresponding Author), Paavo Rahkila, Nuno Fachada, Tuomas Turpeinen, Urho Kujala, Heikki Kainulainen

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.
Original languageEnglish
Article number151869
JournalActa Histochemica
Volume124
Issue number3
DOIs
Publication statusPublished - Apr 2022
MoE publication typeA1 Journal article-refereed

Keywords

  • Lipid droplets
  • PLIN5
  • Type II diabetes
  • Skeletal muscle
  • Insulin resistance
  • Fiber type

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