Abstract
Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.
| Original language | English |
|---|---|
| Article number | 151869 |
| Journal | Acta Histochemica |
| Volume | 124 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 2022 |
| MoE publication type | A1 Journal article-refereed |
Funding
This study was supported by the Academy of Finland (grant 132987 and 298875 ) and Fundação para a Ciência e a Tecnologia (FCT, Portugal) under grant UIDB/04111/2020 (COPELABS). The author Vasco Fachada acknowledges his FCT grant SFRH/BD/68308/2010.
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Lipid droplets
- PLIN5
- Type II diabetes
- Skeletal muscle
- Insulin resistance
- Fiber type
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