Enumeration and identification of Lactobacillus paracasei subsp. paracasei F19

Stina Björneholm, Annelie Eklöw, Maria Saarela, Jaana Mättö

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

The identification of probiotic lactobacilli at strain level is important for their industrial use. Methods for enumeration and identification of Lactobacillus paracasei subsp. paracasei isolate F19 ( Lactobacillus F19) were evaluated. Nine closely related isolates were used to validate the methods. MRS with vancomycin (20 &#119 g ml) or adjusted to pH 5.4, and Rogosa with vancomycin (20 &#119 g ml) showed good results for enumeration in dairy products and biological samples, respectively. MRS with cefotaxime (0.5-2 &#119 g ml) slightly suppressed growth and cell count of Lactobacillus F19. RAPD analysis modified from the study by Tynkkynen et al. (1999) proved applicable for strain-level identification of Lactobacillus F19. Also, FT-IR analysis was tested with good results. The recommendation is to enumerate using the MRS with vancomycin or adjusted to pH 5.4 and screen colonies with RAPD analysis with the primer Lbc-19 and confirm identity with the primer OPA-02, provided that the preparation of crude cell extracts and the parameters of RAPD protocol are strictly standardized.
Original languageEnglish
Pages (from-to)7-13
JournalMicrobial Ecology in Health and Disease
Volume14
Issue number1
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

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Lactobacillus
Vancomycin
Dairy Products
Cefotaxime
Probiotics
Cell Extracts
Complex Mixtures
Cell Count
Lactobacillus paracasei
Growth

Keywords

  • lactobacillus
  • paracasei
  • identification
  • cefotaxime
  • vancomycin
  • rapd

Cite this

Björneholm, Stina ; Eklöw, Annelie ; Saarela, Maria ; Mättö, Jaana. / Enumeration and identification of Lactobacillus paracasei subsp. paracasei F19. In: Microbial Ecology in Health and Disease. 2002 ; Vol. 14, No. 1. pp. 7-13.
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abstract = "The identification of probiotic lactobacilli at strain level is important for their industrial use. Methods for enumeration and identification of Lactobacillus paracasei subsp. paracasei isolate F19 ( Lactobacillus F19) were evaluated. Nine closely related isolates were used to validate the methods. MRS with vancomycin (20 &#119 g ml) or adjusted to pH 5.4, and Rogosa with vancomycin (20 &#119 g ml) showed good results for enumeration in dairy products and biological samples, respectively. MRS with cefotaxime (0.5-2 &#119 g ml) slightly suppressed growth and cell count of Lactobacillus F19. RAPD analysis modified from the study by Tynkkynen et al. (1999) proved applicable for strain-level identification of Lactobacillus F19. Also, FT-IR analysis was tested with good results. The recommendation is to enumerate using the MRS with vancomycin or adjusted to pH 5.4 and screen colonies with RAPD analysis with the primer Lbc-19 and confirm identity with the primer OPA-02, provided that the preparation of crude cell extracts and the parameters of RAPD protocol are strictly standardized.",
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Enumeration and identification of Lactobacillus paracasei subsp. paracasei F19. / Björneholm, Stina; Eklöw, Annelie; Saarela, Maria; Mättö, Jaana.

In: Microbial Ecology in Health and Disease, Vol. 14, No. 1, 2002, p. 7-13.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enumeration and identification of Lactobacillus paracasei subsp. paracasei F19

AU - Björneholm, Stina

AU - Eklöw, Annelie

AU - Saarela, Maria

AU - Mättö, Jaana

PY - 2002

Y1 - 2002

N2 - The identification of probiotic lactobacilli at strain level is important for their industrial use. Methods for enumeration and identification of Lactobacillus paracasei subsp. paracasei isolate F19 ( Lactobacillus F19) were evaluated. Nine closely related isolates were used to validate the methods. MRS with vancomycin (20 &#119 g ml) or adjusted to pH 5.4, and Rogosa with vancomycin (20 &#119 g ml) showed good results for enumeration in dairy products and biological samples, respectively. MRS with cefotaxime (0.5-2 &#119 g ml) slightly suppressed growth and cell count of Lactobacillus F19. RAPD analysis modified from the study by Tynkkynen et al. (1999) proved applicable for strain-level identification of Lactobacillus F19. Also, FT-IR analysis was tested with good results. The recommendation is to enumerate using the MRS with vancomycin or adjusted to pH 5.4 and screen colonies with RAPD analysis with the primer Lbc-19 and confirm identity with the primer OPA-02, provided that the preparation of crude cell extracts and the parameters of RAPD protocol are strictly standardized.

AB - The identification of probiotic lactobacilli at strain level is important for their industrial use. Methods for enumeration and identification of Lactobacillus paracasei subsp. paracasei isolate F19 ( Lactobacillus F19) were evaluated. Nine closely related isolates were used to validate the methods. MRS with vancomycin (20 &#119 g ml) or adjusted to pH 5.4, and Rogosa with vancomycin (20 &#119 g ml) showed good results for enumeration in dairy products and biological samples, respectively. MRS with cefotaxime (0.5-2 &#119 g ml) slightly suppressed growth and cell count of Lactobacillus F19. RAPD analysis modified from the study by Tynkkynen et al. (1999) proved applicable for strain-level identification of Lactobacillus F19. Also, FT-IR analysis was tested with good results. The recommendation is to enumerate using the MRS with vancomycin or adjusted to pH 5.4 and screen colonies with RAPD analysis with the primer Lbc-19 and confirm identity with the primer OPA-02, provided that the preparation of crude cell extracts and the parameters of RAPD protocol are strictly standardized.

KW - lactobacillus

KW - paracasei

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KW - vancomycin

KW - rapd

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