Enzymatic accessibility of xylans in lignocellulosic materials

Liisa Viikari, Anne Kantelinen, Johanna Buchert, Jurgen Puls

Research output: Contribution to journalArticleScientificpeer-review

51 Citations (Scopus)

Abstract

The hydrolysis of fibre-bound and isolated xylans from both birch and pine wood and kraft pulps was studied using purified xylanolytic enzymes of Trichoderma reesei. Despite high enzyme loading, the degree of hydrolysis of fibre-bound substrates did not exceed 20% of the theoretical value, apparently due to limited accessibility of the substrates. The fibre-bound xylans were as equally accessible in softwood as in hardwood pulps. The isolated xylans of wood and kraft pulps could be solubilized more extensively, with a hydrolysis yield of 50–65%. The substitution degree of the isolated xylan substrates was reflected in the different hydrolysis yields obtained by the two xylanases, with isoelectric point (pI) values of 9.0 and 5.5. On the more substituted substrates, i.e. pine kraft xylan and pine wood xylan, the two enzymes acted almost similarly, whereas on the less substituted xylan substrates, such as isolated birch kraft xylan, the pI-9.0 enzyme was more efficient. The side-group-cleaving enzymes increased only moderately the solubilization of the substrates.
Original languageEnglish
Pages (from-to)124-129
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume41
Issue number1
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

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Xylans
Hydrolysis
Enzymes
Betula
Trichoderma
Isoelectric Point

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Viikari, Liisa ; Kantelinen, Anne ; Buchert, Johanna ; Puls, Jurgen. / Enzymatic accessibility of xylans in lignocellulosic materials. In: Applied Microbiology and Biotechnology. 1994 ; Vol. 41, No. 1. pp. 124-129.
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title = "Enzymatic accessibility of xylans in lignocellulosic materials",
abstract = "The hydrolysis of fibre-bound and isolated xylans from both birch and pine wood and kraft pulps was studied using purified xylanolytic enzymes of Trichoderma reesei. Despite high enzyme loading, the degree of hydrolysis of fibre-bound substrates did not exceed 20{\%} of the theoretical value, apparently due to limited accessibility of the substrates. The fibre-bound xylans were as equally accessible in softwood as in hardwood pulps. The isolated xylans of wood and kraft pulps could be solubilized more extensively, with a hydrolysis yield of 50–65{\%}. The substitution degree of the isolated xylan substrates was reflected in the different hydrolysis yields obtained by the two xylanases, with isoelectric point (pI) values of 9.0 and 5.5. On the more substituted substrates, i.e. pine kraft xylan and pine wood xylan, the two enzymes acted almost similarly, whereas on the less substituted xylan substrates, such as isolated birch kraft xylan, the pI-9.0 enzyme was more efficient. The side-group-cleaving enzymes increased only moderately the solubilization of the substrates.",
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Enzymatic accessibility of xylans in lignocellulosic materials. / Viikari, Liisa; Kantelinen, Anne; Buchert, Johanna; Puls, Jurgen.

In: Applied Microbiology and Biotechnology, Vol. 41, No. 1, 1994, p. 124-129.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzymatic accessibility of xylans in lignocellulosic materials

AU - Viikari, Liisa

AU - Kantelinen, Anne

AU - Buchert, Johanna

AU - Puls, Jurgen

N1 - Project code: VBIO2022

PY - 1994

Y1 - 1994

N2 - The hydrolysis of fibre-bound and isolated xylans from both birch and pine wood and kraft pulps was studied using purified xylanolytic enzymes of Trichoderma reesei. Despite high enzyme loading, the degree of hydrolysis of fibre-bound substrates did not exceed 20% of the theoretical value, apparently due to limited accessibility of the substrates. The fibre-bound xylans were as equally accessible in softwood as in hardwood pulps. The isolated xylans of wood and kraft pulps could be solubilized more extensively, with a hydrolysis yield of 50–65%. The substitution degree of the isolated xylan substrates was reflected in the different hydrolysis yields obtained by the two xylanases, with isoelectric point (pI) values of 9.0 and 5.5. On the more substituted substrates, i.e. pine kraft xylan and pine wood xylan, the two enzymes acted almost similarly, whereas on the less substituted xylan substrates, such as isolated birch kraft xylan, the pI-9.0 enzyme was more efficient. The side-group-cleaving enzymes increased only moderately the solubilization of the substrates.

AB - The hydrolysis of fibre-bound and isolated xylans from both birch and pine wood and kraft pulps was studied using purified xylanolytic enzymes of Trichoderma reesei. Despite high enzyme loading, the degree of hydrolysis of fibre-bound substrates did not exceed 20% of the theoretical value, apparently due to limited accessibility of the substrates. The fibre-bound xylans were as equally accessible in softwood as in hardwood pulps. The isolated xylans of wood and kraft pulps could be solubilized more extensively, with a hydrolysis yield of 50–65%. The substitution degree of the isolated xylan substrates was reflected in the different hydrolysis yields obtained by the two xylanases, with isoelectric point (pI) values of 9.0 and 5.5. On the more substituted substrates, i.e. pine kraft xylan and pine wood xylan, the two enzymes acted almost similarly, whereas on the less substituted xylan substrates, such as isolated birch kraft xylan, the pI-9.0 enzyme was more efficient. The side-group-cleaving enzymes increased only moderately the solubilization of the substrates.

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