Enzymatic degradation of and bovine serum albumin release from starch-acetate films

Laura Tuovinen (Corresponding Author), Soili Peltonen, Tapani Suortti, Nicholas Crowther, Matti Elomaa, Kristiina Järvinen

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

The effect of acetylation of potato starch on swelling, enzymatic degradation, and bovine serum albumin (BSA, molecular mass 68 kDa) release rate from polymer films was studied. Potato starch and potato starch acetates (SA), having a degree of substitution of 1.9 or 2.6, were investigated. Polymer films were incubated in phosphate buffer solution pH 7.4 in the absence and presence of enzymes (-amylase, amyloglucosidase, esterase) or in human serum. The acetylation of potato starch decreased its swelling considerably. Increased acetylation of starch also considerably retarded its enzymatic degradation. Due to the decreased swelling and degradation of SA films, BSA was released much slower from SA films than from potato starch films, both in the presence and absence of enzymes.
Original languageEnglish
Pages (from-to)284-290
JournalBiomacromolecules
Volume3
Issue number2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Bovine Serum Albumin
Starch
Acetylation
Degradation
Swelling
Polymer films
Glucan 1,4-alpha-Glucosidase
Molecular mass
Esterases
Enzymes
Buffers
Substitution reactions
Phosphates
starch acetate

Keywords

  • biodegradation
  • polymers
  • starch
  • potato
  • pharmaceutical compounds
  • drug delivery systems
  • amylose
  • amylopectin
  • acetylation

Cite this

Tuovinen, L., Peltonen, S., Suortti, T., Crowther, N., Elomaa, M., & Järvinen, K. (2002). Enzymatic degradation of and bovine serum albumin release from starch-acetate films. Biomacromolecules, 3(2), 284-290. https://doi.org/10.1021/bm015581e
Tuovinen, Laura ; Peltonen, Soili ; Suortti, Tapani ; Crowther, Nicholas ; Elomaa, Matti ; Järvinen, Kristiina. / Enzymatic degradation of and bovine serum albumin release from starch-acetate films. In: Biomacromolecules. 2002 ; Vol. 3, No. 2. pp. 284-290.
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abstract = "The effect of acetylation of potato starch on swelling, enzymatic degradation, and bovine serum albumin (BSA, molecular mass 68 kDa) release rate from polymer films was studied. Potato starch and potato starch acetates (SA), having a degree of substitution of 1.9 or 2.6, were investigated. Polymer films were incubated in phosphate buffer solution pH 7.4 in the absence and presence of enzymes (-amylase, amyloglucosidase, esterase) or in human serum. The acetylation of potato starch decreased its swelling considerably. Increased acetylation of starch also considerably retarded its enzymatic degradation. Due to the decreased swelling and degradation of SA films, BSA was released much slower from SA films than from potato starch films, both in the presence and absence of enzymes.",
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Tuovinen, L, Peltonen, S, Suortti, T, Crowther, N, Elomaa, M & Järvinen, K 2002, 'Enzymatic degradation of and bovine serum albumin release from starch-acetate films', Biomacromolecules, vol. 3, no. 2, pp. 284-290. https://doi.org/10.1021/bm015581e

Enzymatic degradation of and bovine serum albumin release from starch-acetate films. / Tuovinen, Laura (Corresponding Author); Peltonen, Soili; Suortti, Tapani; Crowther, Nicholas; Elomaa, Matti; Järvinen, Kristiina.

In: Biomacromolecules, Vol. 3, No. 2, 2002, p. 284-290.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzymatic degradation of and bovine serum albumin release from starch-acetate films

AU - Tuovinen, Laura

AU - Peltonen, Soili

AU - Suortti, Tapani

AU - Crowther, Nicholas

AU - Elomaa, Matti

AU - Järvinen, Kristiina

PY - 2002

Y1 - 2002

N2 - The effect of acetylation of potato starch on swelling, enzymatic degradation, and bovine serum albumin (BSA, molecular mass 68 kDa) release rate from polymer films was studied. Potato starch and potato starch acetates (SA), having a degree of substitution of 1.9 or 2.6, were investigated. Polymer films were incubated in phosphate buffer solution pH 7.4 in the absence and presence of enzymes (-amylase, amyloglucosidase, esterase) or in human serum. The acetylation of potato starch decreased its swelling considerably. Increased acetylation of starch also considerably retarded its enzymatic degradation. Due to the decreased swelling and degradation of SA films, BSA was released much slower from SA films than from potato starch films, both in the presence and absence of enzymes.

AB - The effect of acetylation of potato starch on swelling, enzymatic degradation, and bovine serum albumin (BSA, molecular mass 68 kDa) release rate from polymer films was studied. Potato starch and potato starch acetates (SA), having a degree of substitution of 1.9 or 2.6, were investigated. Polymer films were incubated in phosphate buffer solution pH 7.4 in the absence and presence of enzymes (-amylase, amyloglucosidase, esterase) or in human serum. The acetylation of potato starch decreased its swelling considerably. Increased acetylation of starch also considerably retarded its enzymatic degradation. Due to the decreased swelling and degradation of SA films, BSA was released much slower from SA films than from potato starch films, both in the presence and absence of enzymes.

KW - biodegradation

KW - polymers

KW - starch

KW - potato

KW - pharmaceutical compounds

KW - drug delivery systems

KW - amylose

KW - amylopectin

KW - acetylation

U2 - 10.1021/bm015581e

DO - 10.1021/bm015581e

M3 - Article

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Tuovinen L, Peltonen S, Suortti T, Crowther N, Elomaa M, Järvinen K. Enzymatic degradation of and bovine serum albumin release from starch-acetate films. Biomacromolecules. 2002;3(2):284-290. https://doi.org/10.1021/bm015581e