Enzymatic degradation of cellulose-based materials

Merja Itävaara, Matti Siika-aho, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)

Abstract

The biodegradability of cellulose-based materials was compared in the standard Sturm test and by enzymatic hydrolysis. Trichoderma reesei culture filtrate, the purified enzymes endoglucanase I and II from T. reesei, and β-glucosidase from Aspergillus niger were used in the experiments. The unpurified Trichoderma reesei culture filtrate was found to contain a mixture of enzymes suitable for cellulose degradation. However, when purified enzymes were used the right balance of the individual enzymes was necessary. The addition of β-glucosidase enhanced the enzymatic hydrolysis of cellulose materials when both culture filtrate and purified enzymes were used. In the Sturm test the biodegradability of most of the cellulose materials exceeded 70% carbon dioxide generation, but, in contrast, the biodegradability of the highly substituted aminated cellulose and cellulose acetate was below 10%. The results concerning enzymatic hydrolysis and biodegradability were in good agreement for kraft paper, sausage casing, aminated cellulose, and cellulose acetate. However, diverging results were obtained with cotton fabric, probably as a result of its high crystallinity.
Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalJournal of Environmental Polymer Degradation
Volume7
Issue number2
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Cellulose
Biodegradability
Degradation
Enzymatic hydrolysis
Enzymes
Glucosidases
Kraft paper
Cellulases
Aspergillus
Cotton fabrics
Carbon Dioxide
Carbon dioxide
Experiments
acetylcellulose

Cite this

Itävaara, Merja ; Siika-aho, Matti ; Viikari, Liisa. / Enzymatic degradation of cellulose-based materials. In: Journal of Environmental Polymer Degradation. 1999 ; Vol. 7, No. 2. pp. 67-73.
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Enzymatic degradation of cellulose-based materials. / Itävaara, Merja; Siika-aho, Matti; Viikari, Liisa.

In: Journal of Environmental Polymer Degradation, Vol. 7, No. 2, 1999, p. 67-73.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Siika-aho, Matti

AU - Viikari, Liisa

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AB - The biodegradability of cellulose-based materials was compared in the standard Sturm test and by enzymatic hydrolysis. Trichoderma reesei culture filtrate, the purified enzymes endoglucanase I and II from T. reesei, and β-glucosidase from Aspergillus niger were used in the experiments. The unpurified Trichoderma reesei culture filtrate was found to contain a mixture of enzymes suitable for cellulose degradation. However, when purified enzymes were used the right balance of the individual enzymes was necessary. The addition of β-glucosidase enhanced the enzymatic hydrolysis of cellulose materials when both culture filtrate and purified enzymes were used. In the Sturm test the biodegradability of most of the cellulose materials exceeded 70% carbon dioxide generation, but, in contrast, the biodegradability of the highly substituted aminated cellulose and cellulose acetate was below 10%. The results concerning enzymatic hydrolysis and biodegradability were in good agreement for kraft paper, sausage casing, aminated cellulose, and cellulose acetate. However, diverging results were obtained with cotton fabric, probably as a result of its high crystallinity.

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