Enzymatic determination of biogenic amines with transglutaminase

Katri Punakivi (Corresponding Author), Maria Smolander, Marja-Leena Niku-Paavola, J. Mattinen, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.
Original languageEnglish
Pages (from-to)1040-1045
Number of pages6
JournalTalanta
Volume68
Issue number3
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Transglutaminases
Biogenic Amines
Ammonia
Substrates
Cadaverine
Food Quality
Tyramine
Putrescine
Histamine
Food

Keywords

  • Tyramine
  • Histamine
  • Putrescine
  • Cadaverine
  • Transglutaminase
  • Detection

Cite this

Punakivi, K., Smolander, M., Niku-Paavola, M-L., Mattinen, J., & Buchert, J. (2006). Enzymatic determination of biogenic amines with transglutaminase. Talanta, 68(3), 1040-1045. https://doi.org/10.1016/j.talanta.2005.06.009
Punakivi, Katri ; Smolander, Maria ; Niku-Paavola, Marja-Leena ; Mattinen, J. ; Buchert, Johanna. / Enzymatic determination of biogenic amines with transglutaminase. In: Talanta. 2006 ; Vol. 68, No. 3. pp. 1040-1045.
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abstract = "Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.",
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Punakivi, K, Smolander, M, Niku-Paavola, M-L, Mattinen, J & Buchert, J 2006, 'Enzymatic determination of biogenic amines with transglutaminase', Talanta, vol. 68, no. 3, pp. 1040-1045. https://doi.org/10.1016/j.talanta.2005.06.009

Enzymatic determination of biogenic amines with transglutaminase. / Punakivi, Katri (Corresponding Author); Smolander, Maria; Niku-Paavola, Marja-Leena; Mattinen, J.; Buchert, Johanna.

In: Talanta, Vol. 68, No. 3, 2006, p. 1040-1045.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzymatic determination of biogenic amines with transglutaminase

AU - Punakivi, Katri

AU - Smolander, Maria

AU - Niku-Paavola, Marja-Leena

AU - Mattinen, J.

AU - Buchert, Johanna

PY - 2006

Y1 - 2006

N2 - Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.

AB - Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.

KW - Tyramine

KW - Histamine

KW - Putrescine

KW - Cadaverine

KW - Transglutaminase

KW - Detection

U2 - 10.1016/j.talanta.2005.06.009

DO - 10.1016/j.talanta.2005.06.009

M3 - Article

VL - 68

SP - 1040

EP - 1045

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 3

ER -