Enzymatic determination of biogenic amines with transglutaminase

Katri Punakivi (Corresponding Author), Maria Smolander, Marja-Leena Niku-Paavola, J. Mattinen, Johanna Buchert

    Research output: Contribution to journalArticleScientificpeer-review

    28 Citations (Scopus)

    Abstract

    Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.
    Original languageEnglish
    Pages (from-to)1040-1045
    Number of pages6
    JournalTalanta
    Volume68
    Issue number3
    DOIs
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Tyramine
    • Histamine
    • Putrescine
    • Cadaverine
    • Transglutaminase
    • Detection

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