Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1040-1045 |
| Number of pages | 6 |
| Journal | Talanta |
| Volume | 68 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Tyramine
- Histamine
- Putrescine
- Cadaverine
- Transglutaminase
- Detection
Cite this
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Enzymatic determination of biogenic amines with transglutaminase. / Punakivi, Katri (Corresponding Author); Smolander, Maria; Niku-Paavola, Marja-Leena; Mattinen, J.; Buchert, Johanna.
In: Talanta, Vol. 68, No. 3, 2006, p. 1040-1045.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Enzymatic determination of biogenic amines with transglutaminase
AU - Punakivi, Katri
AU - Smolander, Maria
AU - Niku-Paavola, Marja-Leena
AU - Mattinen, J.
AU - Buchert, Johanna
PY - 2006
Y1 - 2006
N2 - Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.
AB - Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.
KW - Tyramine
KW - Histamine
KW - Putrescine
KW - Cadaverine
KW - Transglutaminase
KW - Detection
U2 - 10.1016/j.talanta.2005.06.009
DO - 10.1016/j.talanta.2005.06.009
M3 - Article
VL - 68
SP - 1040
EP - 1045
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 3
ER -