Enzymatic determination of biogenic amines with transglutaminase

Katri Punakivi (Corresponding Author), Maria Smolander, Marja-Leena Niku-Paavola, J. Mattinen, Johanna Buchert

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26 Citations (Scopus)

Abstract

Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.
Original languageEnglish
Pages (from-to)1040-1045
Number of pages6
JournalTalanta
Volume68
Issue number3
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Keywords

  • Tyramine
  • Histamine
  • Putrescine
  • Cadaverine
  • Transglutaminase
  • Detection

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    Punakivi, K., Smolander, M., Niku-Paavola, M-L., Mattinen, J., & Buchert, J. (2006). Enzymatic determination of biogenic amines with transglutaminase. Talanta, 68(3), 1040-1045. https://doi.org/10.1016/j.talanta.2005.06.009