Enzymatic determination of biogenic amines with transglutaminase

Katri Punakivi; Maria Smolander; Marja-Leena Niku-Paavola; J. Mattinen; Johanna Buchert

doi:10.1016/j.talanta.2005.06.009

Enzymatic determination of biogenic amines with transglutaminase

Katri Punakivi^*
, Maria Smolander
, Marja-Leena Niku-Paavola
, J. Mattinen
, Johanna Buchert

^*Corresponding author for this work

ENEA - Ricerca sul Sistema Elettrico (ERSE) SpA.
Åbo Akademi University

Research output: Contribution to journal › Article › Scientific › peer-review

31 Citations (Scopus)

Abstract

Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.

Original language	English
Pages (from-to)	1040-1045
Number of pages	6
Journal	Talanta
Volume	68
Issue number	3
DOIs	https://doi.org/10.1016/j.talanta.2005.06.009
Publication status	Published - 2006
MoE publication type	A1 Journal article-refereed

Keywords

Tyramine
Histamine
Putrescine
Cadaverine
Transglutaminase
Detection

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10.1016/j.talanta.2005.06.009

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title = "Enzymatic determination of biogenic amines with transglutaminase",

abstract = "Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.",

keywords = "Tyramine, Histamine, Putrescine, Cadaverine, Transglutaminase, Detection",

author = "Katri Punakivi and Maria Smolander and Marja-Leena Niku-Paavola and J. Mattinen and Johanna Buchert",

year = "2006",

doi = "10.1016/j.talanta.2005.06.009",

language = "English",

volume = "68",

pages = "1040--1045",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - Enzymatic determination of biogenic amines with transglutaminase

AU - Punakivi, Katri

AU - Smolander, Maria

AU - Niku-Paavola, Marja-Leena

AU - Mattinen, J.

AU - Buchert, Johanna

PY - 2006

Y1 - 2006

N2 - Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.

AB - Tyramine, histamine, putrescine and cadaverine, the most common biogenic amines indicating the food quality, were studied in the transglutaminase-catalyzed reaction. Transglutaminase (protein-glutamine gamma-glutamyltransferase EC 2.3.2.13) catalyzes an acyl transfer reaction between a donor substrate and an acceptor substrate (e.g. biogenic amine) and forms a cross-linkage between substrates with a release of ammonia. The reaction can be monitored by measuring the ammonia produced in the reaction. The concentration of produced ammonia was found to be proportional to the concentration of biogenic amine and could hence be used to determination of biogenic amines in food matrixes.

KW - Tyramine

KW - Histamine

KW - Putrescine

KW - Cadaverine

KW - Transglutaminase

KW - Detection

U2 - 10.1016/j.talanta.2005.06.009

DO - 10.1016/j.talanta.2005.06.009

M3 - Article

SN - 0039-9140

VL - 68

SP - 1040

EP - 1045

JO - Talanta

JF - Talanta

IS - 3

ER -

Enzymatic determination of biogenic amines with transglutaminase

Abstract

Keywords

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