TY - JOUR
T1 - Enzymatic solubilization of brewers' spent grain by combined action of carbohydrases and peptidases
AU - Treimo, Janneke
AU - Westereng, Bjorge
AU - Horn, Svein J.
AU - Forssell, Pirkko
AU - Robertson, James A.
AU - Faulds, Craig B.
AU - Waldron, Keith W.
AU - Buchert, Johanna
AU - Eijsink, Vincent G. H.
PY - 2009
Y1 - 2009
N2 - Brewers’ spent grain (BSG), a high-volume coproduct from the brewing
industry, primarily contains proteins, barley cell wall carbohydrates,
and lignin. To create new possibilities for the exploitation of this
large biomass stream, the solubilization of BSG by the combined action
of carbohydrases (Depol 740 and Econase) and peptidase (Alcalase and
Promod 439) was explored. Hydrolysis protocols were optimized with
respect to temperature (influencing both microbial contamination and
rate of enzymatic hydrolysis), pH, enzyme dose, order of enzyme
addition, and processing time. On the basis of this approach, one- and
two-step protocols are proposed taking 4−8 h and yielding combined or
separate fractions of hydrolyzed oligosaccharides and liberated
hydrolyzed protein. Optimized procedures resulted in the solubilization
of >80% of the proteinaceous material, up to 39% of the total
carbohydrates, and up to 42% of total dry matter in BSG. Of the original
xylan present in BSG, 36% could be solubilized. Sequential and
simultaneous treatments with the two enzyme types gave similar results.
In sequential processes, the order of the carbohydrase and peptidase
treatments had only minor effects on the outcome. Depol 740 released
more pentoses than Econase and gave slightly higher overall dry matter
solubilization yields.
AB - Brewers’ spent grain (BSG), a high-volume coproduct from the brewing
industry, primarily contains proteins, barley cell wall carbohydrates,
and lignin. To create new possibilities for the exploitation of this
large biomass stream, the solubilization of BSG by the combined action
of carbohydrases (Depol 740 and Econase) and peptidase (Alcalase and
Promod 439) was explored. Hydrolysis protocols were optimized with
respect to temperature (influencing both microbial contamination and
rate of enzymatic hydrolysis), pH, enzyme dose, order of enzyme
addition, and processing time. On the basis of this approach, one- and
two-step protocols are proposed taking 4−8 h and yielding combined or
separate fractions of hydrolyzed oligosaccharides and liberated
hydrolyzed protein. Optimized procedures resulted in the solubilization
of >80% of the proteinaceous material, up to 39% of the total
carbohydrates, and up to 42% of total dry matter in BSG. Of the original
xylan present in BSG, 36% could be solubilized. Sequential and
simultaneous treatments with the two enzyme types gave similar results.
In sequential processes, the order of the carbohydrase and peptidase
treatments had only minor effects on the outcome. Depol 740 released
more pentoses than Econase and gave slightly higher overall dry matter
solubilization yields.
U2 - 10.1021/jf803310f
DO - 10.1021/jf803310f
M3 - Article
SN - 0021-8561
VL - 57
SP - 3316
EP - 3324
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 8
ER -