Enzymatic solubilization of brewers' spent grain by combined action of carbohydrases and peptidases

Janneke Treimo (Corresponding Author), Bjorge Westereng, Svein J. Horn, Pirkko Forssell, James A. Robertson, Craig B. Faulds, Keith W. Waldron, Johanna Buchert, Vincent G. H. Eijsink

Research output: Contribution to journalArticleScientificpeer-review

34 Citations (Scopus)

Abstract

Brewers’ spent grain (BSG), a high-volume coproduct from the brewing industry, primarily contains proteins, barley cell wall carbohydrates, and lignin. To create new possibilities for the exploitation of this large biomass stream, the solubilization of BSG by the combined action of carbohydrases (Depol 740 and Econase) and peptidase (Alcalase and Promod 439) was explored. Hydrolysis protocols were optimized with respect to temperature (influencing both microbial contamination and rate of enzymatic hydrolysis), pH, enzyme dose, order of enzyme addition, and processing time. On the basis of this approach, one- and two-step protocols are proposed taking 4−8 h and yielding combined or separate fractions of hydrolyzed oligosaccharides and liberated hydrolyzed protein. Optimized procedures resulted in the solubilization of >80% of the proteinaceous material, up to 39% of the total carbohydrates, and up to 42% of total dry matter in BSG. Of the original xylan present in BSG, 36% could be solubilized. Sequential and simultaneous treatments with the two enzyme types gave similar results. In sequential processes, the order of the carbohydrase and peptidase treatments had only minor effects on the outcome. Depol 740 released more pentoses than Econase and gave slightly higher overall dry matter solubilization yields.
Original languageEnglish
Pages (from-to)3316-3324
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number8
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

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spent grains
brewers grains
peptidases
solubilization
Peptide Hydrolases
Hydrolysis
Enzymes
Carbohydrates
Subtilisins
Pentoses
Xylans
enzymes
Lignin
Hordeum
Brewing
barley protein
carbohydrates
Oligosaccharides
Biomass
Cell Wall

Cite this

Treimo, J., Westereng, B., Horn, S. J., Forssell, P., Robertson, J. A., Faulds, C. B., ... Eijsink, V. G. H. (2009). Enzymatic solubilization of brewers' spent grain by combined action of carbohydrases and peptidases. Journal of Agricultural and Food Chemistry, 57(8), 3316-3324. https://doi.org/10.1021/jf803310f
Treimo, Janneke ; Westereng, Bjorge ; Horn, Svein J. ; Forssell, Pirkko ; Robertson, James A. ; Faulds, Craig B. ; Waldron, Keith W. ; Buchert, Johanna ; Eijsink, Vincent G. H. / Enzymatic solubilization of brewers' spent grain by combined action of carbohydrases and peptidases. In: Journal of Agricultural and Food Chemistry. 2009 ; Vol. 57, No. 8. pp. 3316-3324.
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abstract = "Brewers’ spent grain (BSG), a high-volume coproduct from the brewing industry, primarily contains proteins, barley cell wall carbohydrates, and lignin. To create new possibilities for the exploitation of this large biomass stream, the solubilization of BSG by the combined action of carbohydrases (Depol 740 and Econase) and peptidase (Alcalase and Promod 439) was explored. Hydrolysis protocols were optimized with respect to temperature (influencing both microbial contamination and rate of enzymatic hydrolysis), pH, enzyme dose, order of enzyme addition, and processing time. On the basis of this approach, one- and two-step protocols are proposed taking 4−8 h and yielding combined or separate fractions of hydrolyzed oligosaccharides and liberated hydrolyzed protein. Optimized procedures resulted in the solubilization of >80{\%} of the proteinaceous material, up to 39{\%} of the total carbohydrates, and up to 42{\%} of total dry matter in BSG. Of the original xylan present in BSG, 36{\%} could be solubilized. Sequential and simultaneous treatments with the two enzyme types gave similar results. In sequential processes, the order of the carbohydrase and peptidase treatments had only minor effects on the outcome. Depol 740 released more pentoses than Econase and gave slightly higher overall dry matter solubilization yields.",
author = "Janneke Treimo and Bjorge Westereng and Horn, {Svein J.} and Pirkko Forssell and Robertson, {James A.} and Faulds, {Craig B.} and Waldron, {Keith W.} and Johanna Buchert and Eijsink, {Vincent G. H.}",
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Treimo, J, Westereng, B, Horn, SJ, Forssell, P, Robertson, JA, Faulds, CB, Waldron, KW, Buchert, J & Eijsink, VGH 2009, 'Enzymatic solubilization of brewers' spent grain by combined action of carbohydrases and peptidases', Journal of Agricultural and Food Chemistry, vol. 57, no. 8, pp. 3316-3324. https://doi.org/10.1021/jf803310f

Enzymatic solubilization of brewers' spent grain by combined action of carbohydrases and peptidases. / Treimo, Janneke (Corresponding Author); Westereng, Bjorge; Horn, Svein J.; Forssell, Pirkko; Robertson, James A.; Faulds, Craig B.; Waldron, Keith W.; Buchert, Johanna; Eijsink, Vincent G. H.

In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 8, 2009, p. 3316-3324.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Enzymatic solubilization of brewers' spent grain by combined action of carbohydrases and peptidases

AU - Treimo, Janneke

AU - Westereng, Bjorge

AU - Horn, Svein J.

AU - Forssell, Pirkko

AU - Robertson, James A.

AU - Faulds, Craig B.

AU - Waldron, Keith W.

AU - Buchert, Johanna

AU - Eijsink, Vincent G. H.

PY - 2009

Y1 - 2009

N2 - Brewers’ spent grain (BSG), a high-volume coproduct from the brewing industry, primarily contains proteins, barley cell wall carbohydrates, and lignin. To create new possibilities for the exploitation of this large biomass stream, the solubilization of BSG by the combined action of carbohydrases (Depol 740 and Econase) and peptidase (Alcalase and Promod 439) was explored. Hydrolysis protocols were optimized with respect to temperature (influencing both microbial contamination and rate of enzymatic hydrolysis), pH, enzyme dose, order of enzyme addition, and processing time. On the basis of this approach, one- and two-step protocols are proposed taking 4−8 h and yielding combined or separate fractions of hydrolyzed oligosaccharides and liberated hydrolyzed protein. Optimized procedures resulted in the solubilization of >80% of the proteinaceous material, up to 39% of the total carbohydrates, and up to 42% of total dry matter in BSG. Of the original xylan present in BSG, 36% could be solubilized. Sequential and simultaneous treatments with the two enzyme types gave similar results. In sequential processes, the order of the carbohydrase and peptidase treatments had only minor effects on the outcome. Depol 740 released more pentoses than Econase and gave slightly higher overall dry matter solubilization yields.

AB - Brewers’ spent grain (BSG), a high-volume coproduct from the brewing industry, primarily contains proteins, barley cell wall carbohydrates, and lignin. To create new possibilities for the exploitation of this large biomass stream, the solubilization of BSG by the combined action of carbohydrases (Depol 740 and Econase) and peptidase (Alcalase and Promod 439) was explored. Hydrolysis protocols were optimized with respect to temperature (influencing both microbial contamination and rate of enzymatic hydrolysis), pH, enzyme dose, order of enzyme addition, and processing time. On the basis of this approach, one- and two-step protocols are proposed taking 4−8 h and yielding combined or separate fractions of hydrolyzed oligosaccharides and liberated hydrolyzed protein. Optimized procedures resulted in the solubilization of >80% of the proteinaceous material, up to 39% of the total carbohydrates, and up to 42% of total dry matter in BSG. Of the original xylan present in BSG, 36% could be solubilized. Sequential and simultaneous treatments with the two enzyme types gave similar results. In sequential processes, the order of the carbohydrase and peptidase treatments had only minor effects on the outcome. Depol 740 released more pentoses than Econase and gave slightly higher overall dry matter solubilization yields.

U2 - 10.1021/jf803310f

DO - 10.1021/jf803310f

M3 - Article

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SP - 3316

EP - 3324

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 8

ER -