Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications

Maija Liisa Mattinen (Corresponding Author), Juan José Valle-Delgado, Timo Leskinen, Tuomas Anttila, Guillaume Riviere, Mika Sipponen, Arja Paananen, Kalle Lintinen, Mauri Kostiainen, Monika Österberg

    Research output: Contribution to journalArticleScientificpeer-review

    63 Citations (Scopus)


    Cross-linked and decolorized lignin nanoparticles (LNPs) were prepared enzymatically and chemically from softwood Kraft lignin. Colloidal lignin particles (CLPs, ca. 200 nm) in a non-malodorous aqueous dispersion could be dried and redispersed in tetrahydrofuran (THF) or in water retaining their stability i.e. spherical shape and size. Two fungal laccases, Trametes hirsuta (ThL) and Melanocarpus albomyces (MaL) were used in the cross-linking reactions. Reactivity of ThL and MaL on Lignoboost™ lignin and LNPs was confirmed by high performance size exclusion chromatography (HPSEC) and oxygen consumption measurements with simultaneous detection of red-brown color due to the formation of quinones. Zeta potential measurements verified oxidation of LNPs via formation of surface-oriented carboxylic acid groups. Dynamic light scattering (DLS) revealed minor changes in the particle size distributions of LNPs after laccase catalyzed radicalization, indicating preferably covalent intraparticular cross-linking over polymerization. Changes in the surface morphology of laccase treated LNPs were imaged by atomic force (AFM) and transmission emission (TEM) microscopy. Furthermore, decolorization of LNPs without degradation was obtained using ultrasonication with H2O2 in alkaline reaction conditions. The research results have high impact for the utilization of Kraft lignin as nanosized colloidal particles in advanced bionanomaterial applications in medicine, foods and cosmetics including different sectors from chemical industry.

    Original languageEnglish
    Pages (from-to)48-56
    Number of pages9
    JournalEnzyme and Microbial Technology
    Publication statusPublished - 1 Apr 2018
    MoE publication typeA1 Journal article-refereed


    • Chemical oxidation
    • Cross-linking
    • Decolorization
    • Laccase
    • Lignin nanoparticle
    • Stabilization


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