Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications

Maija Liisa Mattinen (Corresponding Author), Juan José Valle-Delgado, Timo Leskinen, Tuomas Anttila, Guillaume Riviere, Mika Sipponen, Arja Paananen, Kalle Lintinen, Mauri Kostiainen, Monika Österberg

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Cross-linked and decolorized lignin nanoparticles (LNPs) were prepared enzymatically and chemically from softwood Kraft lignin. Colloidal lignin particles (CLPs, ca. 200 nm) in a non-malodorous aqueous dispersion could be dried and redispersed in tetrahydrofuran (THF) or in water retaining their stability i.e. spherical shape and size. Two fungal laccases, Trametes hirsuta (ThL) and Melanocarpus albomyces (MaL) were used in the cross-linking reactions. Reactivity of ThL and MaL on Lignoboost™ lignin and LNPs was confirmed by high performance size exclusion chromatography (HPSEC) and oxygen consumption measurements with simultaneous detection of red-brown color due to the formation of quinones. Zeta potential measurements verified oxidation of LNPs via formation of surface-oriented carboxylic acid groups. Dynamic light scattering (DLS) revealed minor changes in the particle size distributions of LNPs after laccase catalyzed radicalization, indicating preferably covalent intraparticular cross-linking over polymerization. Changes in the surface morphology of laccase treated LNPs were imaged by atomic force (AFM) and transmission emission (TEM) microscopy. Furthermore, decolorization of LNPs without degradation was obtained using ultrasonication with H2O2 in alkaline reaction conditions. The research results have high impact for the utilization of Kraft lignin as nanosized colloidal particles in advanced bionanomaterial applications in medicine, foods and cosmetics including different sectors from chemical industry.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalEnzyme and Microbial Technology
Volume111
DOIs
Publication statusPublished - 1 Apr 2018
MoE publication typeA1 Journal article-refereed

Fingerprint

Lignin
Biocompatible Materials
Biomaterials
Nanoparticles
Laccase
Trametes
Chemical Industry
Quinones
Size exclusion chromatography
Cosmetics
Softwoods
Cross Reactions
Dynamic light scattering
Zeta potential
Chemical industry
Carboxylic Acids
Particle Size
Oxygen Consumption
Particle size analysis
Polymerization

Keywords

  • Chemical oxidation
  • Cross-linking
  • Decolorization
  • Laccase
  • Lignin nanoparticle
  • Stabilization

Cite this

Mattinen, M. L., Valle-Delgado, J. J., Leskinen, T., Anttila, T., Riviere, G., Sipponen, M., ... Österberg, M. (2018). Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications. Enzyme and Microbial Technology, 111, 48-56. https://doi.org/10.1016/j.enzmictec.2018.01.005
Mattinen, Maija Liisa ; Valle-Delgado, Juan José ; Leskinen, Timo ; Anttila, Tuomas ; Riviere, Guillaume ; Sipponen, Mika ; Paananen, Arja ; Lintinen, Kalle ; Kostiainen, Mauri ; Österberg, Monika. / Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications. In: Enzyme and Microbial Technology. 2018 ; Vol. 111. pp. 48-56.
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Mattinen, ML, Valle-Delgado, JJ, Leskinen, T, Anttila, T, Riviere, G, Sipponen, M, Paananen, A, Lintinen, K, Kostiainen, M & Österberg, M 2018, 'Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications', Enzyme and Microbial Technology, vol. 111, pp. 48-56. https://doi.org/10.1016/j.enzmictec.2018.01.005

Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications. / Mattinen, Maija Liisa (Corresponding Author); Valle-Delgado, Juan José; Leskinen, Timo; Anttila, Tuomas; Riviere, Guillaume; Sipponen, Mika; Paananen, Arja; Lintinen, Kalle; Kostiainen, Mauri; Österberg, Monika.

In: Enzyme and Microbial Technology, Vol. 111, 01.04.2018, p. 48-56.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzymatically and chemically oxidized lignin nanoparticles for biomaterial applications

AU - Mattinen, Maija Liisa

AU - Valle-Delgado, Juan José

AU - Leskinen, Timo

AU - Anttila, Tuomas

AU - Riviere, Guillaume

AU - Sipponen, Mika

AU - Paananen, Arja

AU - Lintinen, Kalle

AU - Kostiainen, Mauri

AU - Österberg, Monika

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Cross-linked and decolorized lignin nanoparticles (LNPs) were prepared enzymatically and chemically from softwood Kraft lignin. Colloidal lignin particles (CLPs, ca. 200 nm) in a non-malodorous aqueous dispersion could be dried and redispersed in tetrahydrofuran (THF) or in water retaining their stability i.e. spherical shape and size. Two fungal laccases, Trametes hirsuta (ThL) and Melanocarpus albomyces (MaL) were used in the cross-linking reactions. Reactivity of ThL and MaL on Lignoboost™ lignin and LNPs was confirmed by high performance size exclusion chromatography (HPSEC) and oxygen consumption measurements with simultaneous detection of red-brown color due to the formation of quinones. Zeta potential measurements verified oxidation of LNPs via formation of surface-oriented carboxylic acid groups. Dynamic light scattering (DLS) revealed minor changes in the particle size distributions of LNPs after laccase catalyzed radicalization, indicating preferably covalent intraparticular cross-linking over polymerization. Changes in the surface morphology of laccase treated LNPs were imaged by atomic force (AFM) and transmission emission (TEM) microscopy. Furthermore, decolorization of LNPs without degradation was obtained using ultrasonication with H2O2 in alkaline reaction conditions. The research results have high impact for the utilization of Kraft lignin as nanosized colloidal particles in advanced bionanomaterial applications in medicine, foods and cosmetics including different sectors from chemical industry.

AB - Cross-linked and decolorized lignin nanoparticles (LNPs) were prepared enzymatically and chemically from softwood Kraft lignin. Colloidal lignin particles (CLPs, ca. 200 nm) in a non-malodorous aqueous dispersion could be dried and redispersed in tetrahydrofuran (THF) or in water retaining their stability i.e. spherical shape and size. Two fungal laccases, Trametes hirsuta (ThL) and Melanocarpus albomyces (MaL) were used in the cross-linking reactions. Reactivity of ThL and MaL on Lignoboost™ lignin and LNPs was confirmed by high performance size exclusion chromatography (HPSEC) and oxygen consumption measurements with simultaneous detection of red-brown color due to the formation of quinones. Zeta potential measurements verified oxidation of LNPs via formation of surface-oriented carboxylic acid groups. Dynamic light scattering (DLS) revealed minor changes in the particle size distributions of LNPs after laccase catalyzed radicalization, indicating preferably covalent intraparticular cross-linking over polymerization. Changes in the surface morphology of laccase treated LNPs were imaged by atomic force (AFM) and transmission emission (TEM) microscopy. Furthermore, decolorization of LNPs without degradation was obtained using ultrasonication with H2O2 in alkaline reaction conditions. The research results have high impact for the utilization of Kraft lignin as nanosized colloidal particles in advanced bionanomaterial applications in medicine, foods and cosmetics including different sectors from chemical industry.

KW - Chemical oxidation

KW - Cross-linking

KW - Decolorization

KW - Laccase

KW - Lignin nanoparticle

KW - Stabilization

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