Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood kraft pulp

Terhi Hakala (Corresponding Author), Tiina Liitiä, Anna Suurnäkki

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

In this paper we describe the effect of enzyme treatments on the production of polymeric xylan, oligosaccharides and hemicellulose lean pulp by alkaline extraction of bleached hardwood kraft pulp. Enzyme treatments were carried out before one or in between two subsequent alkaline extractions by purified Trichoderma reesei xylanase and endoglucanase II (Cel 5a) as well as by a commercial monocomponent endoglucanase (FibreCareR). Without enzyme pre-treatment 61% and 7% of the pulp xylan was extracted in high purity in the first and second alkaline stage, respectively. Higher molecular mass xylan was obtained in the second than in the first alkaline extraction. Xylanase treatment before alkaline extraction hydrolyzed up to 12% of xylan to xylooligosaccharides. According to our results, preparation of polymeric xylan, and/or oligosaccharides as well as hemicellulose lean pulp with cellulose content of 93-94%, is possible by enzyme-aided alkaline extraction process
Original languageEnglish
Pages (from-to)102-108
JournalCarbohydrate Polymers
Volume93
Issue number1
DOIs
Publication statusPublished - 2013
MoE publication typeNot Eligible

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Xylans
Oligosaccharides
Kraft pulp
Hardwoods
Enzymes
Pulp
Cellulase
Molecular mass
Cellulose

Keywords

  • Dissolving pulp
  • endoglucanase
  • enzyme treatment
  • oligosaccharide
  • xylan
  • xylanase

Cite this

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title = "Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood kraft pulp",
abstract = "In this paper we describe the effect of enzyme treatments on the production of polymeric xylan, oligosaccharides and hemicellulose lean pulp by alkaline extraction of bleached hardwood kraft pulp. Enzyme treatments were carried out before one or in between two subsequent alkaline extractions by purified Trichoderma reesei xylanase and endoglucanase II (Cel 5a) as well as by a commercial monocomponent endoglucanase (FibreCareR). Without enzyme pre-treatment 61{\%} and 7{\%} of the pulp xylan was extracted in high purity in the first and second alkaline stage, respectively. Higher molecular mass xylan was obtained in the second than in the first alkaline extraction. Xylanase treatment before alkaline extraction hydrolyzed up to 12{\%} of xylan to xylooligosaccharides. According to our results, preparation of polymeric xylan, and/or oligosaccharides as well as hemicellulose lean pulp with cellulose content of 93-94{\%}, is possible by enzyme-aided alkaline extraction process",
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journal = "Carbohydrate Polymers",
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Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood kraft pulp. / Hakala, Terhi (Corresponding Author); Liitiä, Tiina; Suurnäkki, Anna.

In: Carbohydrate Polymers, Vol. 93, No. 1, 2013, p. 102-108.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood kraft pulp

AU - Hakala, Terhi

AU - Liitiä, Tiina

AU - Suurnäkki, Anna

PY - 2013

Y1 - 2013

N2 - In this paper we describe the effect of enzyme treatments on the production of polymeric xylan, oligosaccharides and hemicellulose lean pulp by alkaline extraction of bleached hardwood kraft pulp. Enzyme treatments were carried out before one or in between two subsequent alkaline extractions by purified Trichoderma reesei xylanase and endoglucanase II (Cel 5a) as well as by a commercial monocomponent endoglucanase (FibreCareR). Without enzyme pre-treatment 61% and 7% of the pulp xylan was extracted in high purity in the first and second alkaline stage, respectively. Higher molecular mass xylan was obtained in the second than in the first alkaline extraction. Xylanase treatment before alkaline extraction hydrolyzed up to 12% of xylan to xylooligosaccharides. According to our results, preparation of polymeric xylan, and/or oligosaccharides as well as hemicellulose lean pulp with cellulose content of 93-94%, is possible by enzyme-aided alkaline extraction process

AB - In this paper we describe the effect of enzyme treatments on the production of polymeric xylan, oligosaccharides and hemicellulose lean pulp by alkaline extraction of bleached hardwood kraft pulp. Enzyme treatments were carried out before one or in between two subsequent alkaline extractions by purified Trichoderma reesei xylanase and endoglucanase II (Cel 5a) as well as by a commercial monocomponent endoglucanase (FibreCareR). Without enzyme pre-treatment 61% and 7% of the pulp xylan was extracted in high purity in the first and second alkaline stage, respectively. Higher molecular mass xylan was obtained in the second than in the first alkaline extraction. Xylanase treatment before alkaline extraction hydrolyzed up to 12% of xylan to xylooligosaccharides. According to our results, preparation of polymeric xylan, and/or oligosaccharides as well as hemicellulose lean pulp with cellulose content of 93-94%, is possible by enzyme-aided alkaline extraction process

KW - Dissolving pulp

KW - endoglucanase

KW - enzyme treatment

KW - oligosaccharide

KW - xylan

KW - xylanase

U2 - 10.1016/j.carbpol.2012.05.013

DO - 10.1016/j.carbpol.2012.05.013

M3 - Article

VL - 93

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JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

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