Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood kraft pulp

  • Terhi Hakala*
  • , Tiina Liitiä
  • , Anna Suurnäkki
  • *Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    70 Citations (Scopus)

    Abstract

    In this paper we describe the effect of enzyme treatments on the production of polymeric xylan, oligosaccharides and hemicellulose lean pulp by alkaline extraction of bleached hardwood kraft pulp. Enzyme treatments were carried out before one or in between two subsequent alkaline extractions by purified Trichoderma reesei xylanase and endoglucanase II (Cel 5a) as well as by a commercial monocomponent endoglucanase (FibreCareR). Without enzyme pre-treatment 61% and 7% of the pulp xylan was extracted in high purity in the first and second alkaline stage, respectively. Higher molecular mass xylan was obtained in the second than in the first alkaline extraction. Xylanase treatment before alkaline extraction hydrolyzed up to 12% of xylan to xylooligosaccharides. According to our results, preparation of polymeric xylan, and/or oligosaccharides as well as hemicellulose lean pulp with cellulose content of 93-94%, is possible by enzyme-aided alkaline extraction process
    Original languageEnglish
    Pages (from-to)102-108
    JournalCarbohydrate Polymers
    Volume93
    Issue number1
    DOIs
    Publication statusPublished - 2013
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Dissolving pulp
    • endoglucanase
    • enzyme treatment
    • oligosaccharide
    • xylan
    • xylanase

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