Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction

Atte Mikkelson, Sari Hyvärinen, Tarja Tamminen, Terhi Hakala

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

Abstract

Galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction was enzymatically hydrolysed into galactoglucomanno-oligosaccharides (GGMOs). Enzymes are known to hydrolyse GGM selectively and depending on the utilized enzyme a different pool of GGMOs can be obtained. The objective of this work was to utilize enzymatic hydrolysis as a tool to estimate carbohydrate pattern of GGM. The galactoglucomannan utilized as substrate in the experiments was isolated from pine wood chips by hot water extraction. The obtained GGM (purity ~73.5%, calculated based on the Man:Glc:Gal ratio of 4:1:0.5) was of relatively low molecular weight (3300 g/mol) and contained pectin and xylan as main impurities (Hyvärinen et al. EWLP 2012)
Original languageEnglish
Title of host publication12th European Workshop on Lignocellulosics and Pulp
Subtitle of host publicationEWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings
EditorsValtteri Mäkelä
Place of PublicationHelsinki
PublisherUniversity of Helsinki
Pages378-381
ISBN (Print)978-952-10-8187-3
Publication statusPublished - 2012
MoE publication typeB3 Non-refereed article in conference proceedings
Event12th European workshop on lignocellulosics and pulp, EWLP 2012 - Espoo, Finland
Duration: 27 Aug 201230 Aug 2012
Conference number: 12

Conference

Conference12th European workshop on lignocellulosics and pulp, EWLP 2012
Abbreviated titleEWLP 2012
CountryFinland
CityEspoo
Period27/08/1230/08/12

Keywords

  • Enzyme
  • hot water extraction
  • analysis

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    Mikkelson, A., Hyvärinen, S., Tamminen, T., & Hakala, T. (2012). Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction. In V. Mäkelä (Ed.), 12th European Workshop on Lignocellulosics and Pulp: EWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings (pp. 378-381). University of Helsinki.