Abstract
Galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction was enzymatically hydrolysed into galactoglucomanno-oligosaccharides (GGMOs). Enzymes are known to hydrolyse GGM selectively and depending on the utilized enzyme a different pool of GGMOs can be obtained. The objective of this work was to utilize enzymatic hydrolysis as a tool to estimate carbohydrate pattern of GGM. The galactoglucomannan utilized as substrate in the experiments was isolated from pine wood chips by hot water extraction. The obtained GGM (purity ~73.5%, calculated based on the Man:Glc:Gal ratio of 4:1:0.5) was of relatively low molecular weight (3300 g/mol) and contained pectin and xylan as main impurities (Hyvärinen et al. EWLP 2012)
| Original language | English |
|---|---|
| Title of host publication | 12th European Workshop on Lignocellulosics and Pulp |
| Subtitle of host publication | EWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings |
| Editors | Valtteri Mäkelä |
| Place of Publication | Helsinki |
| Publisher | University of Helsinki |
| Pages | 378-381 |
| ISBN (Print) | 978-952-10-8187-3 |
| Publication status | Published - 2012 |
| MoE publication type | B3 Non-refereed article in conference proceedings |
| Event | 12th European workshop on lignocellulosics and pulp, EWLP 2012 - Espoo, Finland Duration: 27 Aug 2012 → 30 Aug 2012 Conference number: 12 |
Conference
| Conference | 12th European workshop on lignocellulosics and pulp, EWLP 2012 |
|---|---|
| Abbreviated title | EWLP 2012 |
| Country | Finland |
| City | Espoo |
| Period | 27/08/12 → 30/08/12 |
Keywords
- Enzyme
- hot water extraction
- analysis
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Cite this
Mikkelson, A., Hyvärinen, S., Tamminen, T., & Hakala, T. (2012). Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction. In V. Mäkelä (Ed.), 12th European Workshop on Lignocellulosics and Pulp: EWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings (pp. 378-381). University of Helsinki.