Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction

Atte Mikkelson, Sari Hyvärinen, Tarja Tamminen, Terhi Hakala

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

    Abstract

    Galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction was enzymatically hydrolysed into galactoglucomanno-oligosaccharides (GGMOs). Enzymes are known to hydrolyse GGM selectively and depending on the utilized enzyme a different pool of GGMOs can be obtained. The objective of this work was to utilize enzymatic hydrolysis as a tool to estimate carbohydrate pattern of GGM. The galactoglucomannan utilized as substrate in the experiments was isolated from pine wood chips by hot water extraction. The obtained GGM (purity ~73.5%, calculated based on the Man:Glc:Gal ratio of 4:1:0.5) was of relatively low molecular weight (3300 g/mol) and contained pectin and xylan as main impurities (Hyvärinen et al. EWLP 2012)
    Original languageEnglish
    Title of host publication12th European Workshop on Lignocellulosics and Pulp
    Subtitle of host publicationEWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings
    EditorsValtteri Mäkelä
    Place of PublicationHelsinki
    PublisherUniversity of Helsinki
    Pages378-381
    ISBN (Print)978-952-10-8187-3
    Publication statusPublished - 2012
    MoE publication typeB3 Non-refereed article in conference proceedings
    Event12th European workshop on lignocellulosics and pulp, EWLP 2012 - Espoo, Finland
    Duration: 27 Aug 201230 Aug 2012
    Conference number: 12

    Conference

    Conference12th European workshop on lignocellulosics and pulp, EWLP 2012
    Abbreviated titleEWLP 2012
    Country/TerritoryFinland
    CityEspoo
    Period27/08/1230/08/12

    Keywords

    • Enzyme
    • hot water extraction
    • analysis

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