Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction

Atte Mikkelson; Sari Hyvärinen; Tarja Tamminen; Terhi Hakala

Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction

Atte Mikkelson
, Sari Hyvärinen
, Tarja Tamminen
, Terhi Hakala

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

Abstract

Galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction was enzymatically hydrolysed into galactoglucomanno-oligosaccharides (GGMOs). Enzymes are known to hydrolyse GGM selectively and depending on the utilized enzyme a different pool of GGMOs can be obtained. The objective of this work was to utilize enzymatic hydrolysis as a tool to estimate carbohydrate pattern of GGM. The galactoglucomannan utilized as substrate in the experiments was isolated from pine wood chips by hot water extraction. The obtained GGM (purity ~73.5%, calculated based on the Man:Glc:Gal ratio of 4:1:0.5) was of relatively low molecular weight (3300 g/mol) and contained pectin and xylan as main impurities (Hyvärinen et al. EWLP 2012)

Original language	English
Title of host publication	12th European Workshop on Lignocellulosics and Pulp
Subtitle of host publication	EWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings
Editors	Valtteri Mäkelä
Place of Publication	Helsinki
Publisher	University of Helsinki
Pages	378-381
ISBN (Print)	978-952-10-8187-3
Publication status	Published - 2012
MoE publication type	B3 Non-refereed article in conference proceedings
Event	12th European workshop on lignocellulosics and pulp, EWLP 2012 - Espoo, Finland Duration: 27 Aug 2012 → 30 Aug 2012 Conference number: 12

Conference

Conference	12th European workshop on lignocellulosics and pulp, EWLP 2012
Abbreviated title	EWLP 2012
Country/Territory	Finland
City	Espoo
Period	27/08/12 → 30/08/12

Keywords

Enzyme
hot water extraction
analysis

Cite this

@inproceedings{6476426069e04fd6b464f3e78f350d10,

title = "Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction",

abstract = "Galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction was enzymatically hydrolysed into galactoglucomanno-oligosaccharides (GGMOs). Enzymes are known to hydrolyse GGM selectively and depending on the utilized enzyme a different pool of GGMOs can be obtained. The objective of this work was to utilize enzymatic hydrolysis as a tool to estimate carbohydrate pattern of GGM. The galactoglucomannan utilized as substrate in the experiments was isolated from pine wood chips by hot water extraction. The obtained GGM (purity \textasciitilde{}73.5\%, calculated based on the Man:Glc:Gal ratio of 4:1:0.5) was of relatively low molecular weight (3300 g/mol) and contained pectin and xylan as main impurities (Hyv{\"a}rinen et al. EWLP 2012)",

keywords = "Enzyme, hot water extraction, analysis",

author = "Atte Mikkelson and Sari Hyv{\"a}rinen and Tarja Tamminen and Terhi Hakala",

note = "Project code: 27110; 12th European workshop on lignocellulosics and pulp, EWLP 2012, EWLP 2012 ; Conference date: 27-08-2012 Through 30-08-2012",

year = "2012",

language = "English",

isbn = "978-952-10-8187-3",

pages = "378--381",

editor = "Valtteri M{\"a}kel{\"a}",

booktitle = "12th European Workshop on Lignocellulosics and Pulp",

publisher = "University of Helsinki",

address = "Finland",

}

Mikkelson, A, Hyvärinen, S, Tamminen, T & Hakala, T 2012, Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction. in V Mäkelä (ed.), 12th European Workshop on Lignocellulosics and Pulp: EWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings. University of Helsinki, Helsinki, pp. 378-381, 12th European workshop on lignocellulosics and pulp, EWLP 2012, Espoo, Finland, 27/08/12.

Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction. / Mikkelson, Atte; Hyvärinen, Sari; Tamminen, Tarja et al.
12th European Workshop on Lignocellulosics and Pulp: EWLP 2012 : August 27-30, 2012, Espoo, Finland : Proceedings. ed. / Valtteri Mäkelä. Helsinki: University of Helsinki, 2012. p. 378-381.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

TY - GEN

T1 - Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction

AU - Mikkelson, Atte

AU - Hyvärinen, Sari

AU - Tamminen, Tarja

AU - Hakala, Terhi

N1 - Conference code: 12

PY - 2012

Y1 - 2012

N2 - Galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction was enzymatically hydrolysed into galactoglucomanno-oligosaccharides (GGMOs). Enzymes are known to hydrolyse GGM selectively and depending on the utilized enzyme a different pool of GGMOs can be obtained. The objective of this work was to utilize enzymatic hydrolysis as a tool to estimate carbohydrate pattern of GGM. The galactoglucomannan utilized as substrate in the experiments was isolated from pine wood chips by hot water extraction. The obtained GGM (purity ~73.5%, calculated based on the Man:Glc:Gal ratio of 4:1:0.5) was of relatively low molecular weight (3300 g/mol) and contained pectin and xylan as main impurities (Hyvärinen et al. EWLP 2012)

AB - Galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction was enzymatically hydrolysed into galactoglucomanno-oligosaccharides (GGMOs). Enzymes are known to hydrolyse GGM selectively and depending on the utilized enzyme a different pool of GGMOs can be obtained. The objective of this work was to utilize enzymatic hydrolysis as a tool to estimate carbohydrate pattern of GGM. The galactoglucomannan utilized as substrate in the experiments was isolated from pine wood chips by hot water extraction. The obtained GGM (purity ~73.5%, calculated based on the Man:Glc:Gal ratio of 4:1:0.5) was of relatively low molecular weight (3300 g/mol) and contained pectin and xylan as main impurities (Hyvärinen et al. EWLP 2012)

KW - Enzyme

KW - hot water extraction

KW - analysis

M3 - Conference article in proceedings

SN - 978-952-10-8187-3

SP - 378

EP - 381

BT - 12th European Workshop on Lignocellulosics and Pulp

A2 - Mäkelä, Valtteri

PB - University of Helsinki

CY - Helsinki

T2 - 12th European workshop on lignocellulosics and pulp, EWLP 2012

Y2 - 27 August 2012 through 30 August 2012

ER -

Enzyme-aided analysis of galactoglucomannan (GGM) isolated from pine wood chips by hot water extraction

Abstract

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Keywords

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