Enzyme-aided fractionation of brewer's spent grains in pilot scale

Pirkko Forssell (Corresponding Author), J. Treimo, V. G. H. Eijsink, Craig Faulds, S. Collins, H. A. Schols, S. W. A. Hinz, Olavi Myllymäki, Tarja Tamminen, J. Zoldners, Kaarina Viljanen, K. W. Waldron, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Brewer's spent grain (BSG) is an important coproduct of the brewing industry and is generally used in animal feed. Recently, there has been considerable research into the use of enzymes to convert BSG into more value-added products. In this study, the efficiency of enzymatic fractionation of freshly produced BSG was studied in pilot scale. Carbohydrate-and protein-degrading enzymes were applied sequentially to produce three fractions: carbohydrate hydrolysate, protein hydrolysate, and a solid lignin-carbohydrate residue. To minimize microbial contamination, processing was performed at 60°C for 4 hr. In all, 14 and 36% of the original dry matter was liberated by carbohydrase and protease, respectively. The carbohydrate-degrading enzyme cocktail produced cellulose- and hemicellulose-derived mono- and oligosaccharides. The protease treatment released peptide-enriched hydrolysate. Approximately half of the original BSG solids were solubilized during the two-stage enzymatic process. Although the protease efficiently released the majority of BSG proteins, the carbohydrate matrix was much less accessible to enzyme attack. The impact of scale-up to pilot scale was compared with previous laboratory-scale studies, and the results were found to be in good agreement.
Original languageEnglish
Pages (from-to)91-99
Number of pages9
JournalJournal of the American Society of Brewing Chemists
Volume69
Issue number2
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

spent grains
brewers grains
fractionation
Carbohydrates
carbohydrates
Enzymes
enzymes
Peptide Hydrolases
proteinases
hydrolysates
Protein Hydrolysates
value-added products
coproducts
brewing industry
Monosaccharides
microbial contamination
Lignin
grain protein
protein hydrolysates
monosaccharides

Keywords

  • Brewer's spent grain
  • Fiber-degrading enzymes
  • Fractionation
  • Hydrolysis residue
  • Proteases

Cite this

Forssell, P., Treimo, J., Eijsink, V. G. H., Faulds, C., Collins, S., Schols, H. A., ... Buchert, J. (2011). Enzyme-aided fractionation of brewer's spent grains in pilot scale. Journal of the American Society of Brewing Chemists, 69(2), 91-99. https://doi.org/10.1094/ASBCJ-2011-0408-01
Forssell, Pirkko ; Treimo, J. ; Eijsink, V. G. H. ; Faulds, Craig ; Collins, S. ; Schols, H. A. ; Hinz, S. W. A. ; Myllymäki, Olavi ; Tamminen, Tarja ; Zoldners, J. ; Viljanen, Kaarina ; Waldron, K. W. ; Buchert, Johanna. / Enzyme-aided fractionation of brewer's spent grains in pilot scale. In: Journal of the American Society of Brewing Chemists. 2011 ; Vol. 69, No. 2. pp. 91-99.
@article{2095ecbdf1a14144a04a98acadf04f4d,
title = "Enzyme-aided fractionation of brewer's spent grains in pilot scale",
abstract = "Brewer's spent grain (BSG) is an important coproduct of the brewing industry and is generally used in animal feed. Recently, there has been considerable research into the use of enzymes to convert BSG into more value-added products. In this study, the efficiency of enzymatic fractionation of freshly produced BSG was studied in pilot scale. Carbohydrate-and protein-degrading enzymes were applied sequentially to produce three fractions: carbohydrate hydrolysate, protein hydrolysate, and a solid lignin-carbohydrate residue. To minimize microbial contamination, processing was performed at 60°C for 4 hr. In all, 14 and 36{\%} of the original dry matter was liberated by carbohydrase and protease, respectively. The carbohydrate-degrading enzyme cocktail produced cellulose- and hemicellulose-derived mono- and oligosaccharides. The protease treatment released peptide-enriched hydrolysate. Approximately half of the original BSG solids were solubilized during the two-stage enzymatic process. Although the protease efficiently released the majority of BSG proteins, the carbohydrate matrix was much less accessible to enzyme attack. The impact of scale-up to pilot scale was compared with previous laboratory-scale studies, and the results were found to be in good agreement.",
keywords = "Brewer's spent grain, Fiber-degrading enzymes, Fractionation, Hydrolysis residue, Proteases",
author = "Pirkko Forssell and J. Treimo and Eijsink, {V. G. H.} and Craig Faulds and S. Collins and Schols, {H. A.} and Hinz, {S. W. A.} and Olavi Myllym{\"a}ki and Tarja Tamminen and J. Zoldners and Kaarina Viljanen and Waldron, {K. W.} and Johanna Buchert",
year = "2011",
doi = "10.1094/ASBCJ-2011-0408-01",
language = "English",
volume = "69",
pages = "91--99",
journal = "Journal of the American Society of Brewing Chemists",
issn = "0361-0470",
number = "2",

}

Forssell, P, Treimo, J, Eijsink, VGH, Faulds, C, Collins, S, Schols, HA, Hinz, SWA, Myllymäki, O, Tamminen, T, Zoldners, J, Viljanen, K, Waldron, KW & Buchert, J 2011, 'Enzyme-aided fractionation of brewer's spent grains in pilot scale', Journal of the American Society of Brewing Chemists, vol. 69, no. 2, pp. 91-99. https://doi.org/10.1094/ASBCJ-2011-0408-01

Enzyme-aided fractionation of brewer's spent grains in pilot scale. / Forssell, Pirkko (Corresponding Author); Treimo, J.; Eijsink, V. G. H.; Faulds, Craig; Collins, S.; Schols, H. A.; Hinz, S. W. A.; Myllymäki, Olavi; Tamminen, Tarja; Zoldners, J.; Viljanen, Kaarina; Waldron, K. W.; Buchert, Johanna.

In: Journal of the American Society of Brewing Chemists, Vol. 69, No. 2, 2011, p. 91-99.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzyme-aided fractionation of brewer's spent grains in pilot scale

AU - Forssell, Pirkko

AU - Treimo, J.

AU - Eijsink, V. G. H.

AU - Faulds, Craig

AU - Collins, S.

AU - Schols, H. A.

AU - Hinz, S. W. A.

AU - Myllymäki, Olavi

AU - Tamminen, Tarja

AU - Zoldners, J.

AU - Viljanen, Kaarina

AU - Waldron, K. W.

AU - Buchert, Johanna

PY - 2011

Y1 - 2011

N2 - Brewer's spent grain (BSG) is an important coproduct of the brewing industry and is generally used in animal feed. Recently, there has been considerable research into the use of enzymes to convert BSG into more value-added products. In this study, the efficiency of enzymatic fractionation of freshly produced BSG was studied in pilot scale. Carbohydrate-and protein-degrading enzymes were applied sequentially to produce three fractions: carbohydrate hydrolysate, protein hydrolysate, and a solid lignin-carbohydrate residue. To minimize microbial contamination, processing was performed at 60°C for 4 hr. In all, 14 and 36% of the original dry matter was liberated by carbohydrase and protease, respectively. The carbohydrate-degrading enzyme cocktail produced cellulose- and hemicellulose-derived mono- and oligosaccharides. The protease treatment released peptide-enriched hydrolysate. Approximately half of the original BSG solids were solubilized during the two-stage enzymatic process. Although the protease efficiently released the majority of BSG proteins, the carbohydrate matrix was much less accessible to enzyme attack. The impact of scale-up to pilot scale was compared with previous laboratory-scale studies, and the results were found to be in good agreement.

AB - Brewer's spent grain (BSG) is an important coproduct of the brewing industry and is generally used in animal feed. Recently, there has been considerable research into the use of enzymes to convert BSG into more value-added products. In this study, the efficiency of enzymatic fractionation of freshly produced BSG was studied in pilot scale. Carbohydrate-and protein-degrading enzymes were applied sequentially to produce three fractions: carbohydrate hydrolysate, protein hydrolysate, and a solid lignin-carbohydrate residue. To minimize microbial contamination, processing was performed at 60°C for 4 hr. In all, 14 and 36% of the original dry matter was liberated by carbohydrase and protease, respectively. The carbohydrate-degrading enzyme cocktail produced cellulose- and hemicellulose-derived mono- and oligosaccharides. The protease treatment released peptide-enriched hydrolysate. Approximately half of the original BSG solids were solubilized during the two-stage enzymatic process. Although the protease efficiently released the majority of BSG proteins, the carbohydrate matrix was much less accessible to enzyme attack. The impact of scale-up to pilot scale was compared with previous laboratory-scale studies, and the results were found to be in good agreement.

KW - Brewer's spent grain

KW - Fiber-degrading enzymes

KW - Fractionation

KW - Hydrolysis residue

KW - Proteases

U2 - 10.1094/ASBCJ-2011-0408-01

DO - 10.1094/ASBCJ-2011-0408-01

M3 - Article

VL - 69

SP - 91

EP - 99

JO - Journal of the American Society of Brewing Chemists

JF - Journal of the American Society of Brewing Chemists

SN - 0361-0470

IS - 2

ER -