Enzyme production by recombinant Trichoderma reesei strains

Jaana M. Uusitalo, K. M. Helena Nevalainen, Anu M. Harkki, Jonathan K.C. Knowles, Merja E. Penttilä

Research output: Contribution to journalArticleScientificpeer-review

78 Citations (Scopus)

Abstract

The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

Original languageEnglish
Pages (from-to)35-49
Number of pages15
JournalJournal of Biotechnology
Volume17
Issue number1
DOIs
Publication statusPublished - 1 Jan 1991
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Enzymes
Cellulose 1,4-beta-Cellobiosidase
Chymosin
Cellulases
Genetic Engineering
Cellulase
Gene Silencing
Proteins
Genetic engineering
Gene encoding
Fungi
Genes

Keywords

  • Cellulase
  • Chymosin
  • Filamentous fungi
  • Heterologous gene expression
  • Trichoderma reesei

Cite this

Uusitalo, Jaana M. ; Helena Nevalainen, K. M. ; Harkki, Anu M. ; Knowles, Jonathan K.C. ; Penttilä, Merja E. / Enzyme production by recombinant Trichoderma reesei strains. In: Journal of Biotechnology. 1991 ; Vol. 17, No. 1. pp. 35-49.
@article{229fb68cf0d04c8591ecc2289576b85a,
title = "Enzyme production by recombinant Trichoderma reesei strains",
abstract = "The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.",
keywords = "Cellulase, Chymosin, Filamentous fungi, Heterologous gene expression, Trichoderma reesei",
author = "Uusitalo, {Jaana M.} and {Helena Nevalainen}, {K. M.} and Harkki, {Anu M.} and Knowles, {Jonathan K.C.} and Penttil{\"a}, {Merja E.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0168-1656(91)90025-Q",
language = "English",
volume = "17",
pages = "35--49",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "1",

}

Enzyme production by recombinant Trichoderma reesei strains. / Uusitalo, Jaana M.; Helena Nevalainen, K. M.; Harkki, Anu M.; Knowles, Jonathan K.C.; Penttilä, Merja E.

In: Journal of Biotechnology, Vol. 17, No. 1, 01.01.1991, p. 35-49.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzyme production by recombinant Trichoderma reesei strains

AU - Uusitalo, Jaana M.

AU - Helena Nevalainen, K. M.

AU - Harkki, Anu M.

AU - Knowles, Jonathan K.C.

AU - Penttilä, Merja E.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

AB - The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

KW - Cellulase

KW - Chymosin

KW - Filamentous fungi

KW - Heterologous gene expression

KW - Trichoderma reesei

UR - http://www.scopus.com/inward/record.url?scp=0025957467&partnerID=8YFLogxK

U2 - 10.1016/0168-1656(91)90025-Q

DO - 10.1016/0168-1656(91)90025-Q

M3 - Article

VL - 17

SP - 35

EP - 49

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1

ER -