Enzyme production by recombinant Trichoderma reesei strains

Jaana M. Uusitalo; K. M. Helena Nevalainen; Anu M. Harkki; Jonathan K.C. Knowles; Merja E. Penttilä

doi:10.1016/0168-1656(91)90025-Q

Enzyme production by recombinant Trichoderma reesei strains

Jaana M. Uusitalo^*, K. M. Helena Nevalainen, Anu M. Harkki, Jonathan K.C. Knowles, Merja E. Penttilä

^*Corresponding author for this work

Alko Inc.

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Abstract

The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

Original language	English
Pages (from-to)	35-49
Number of pages	15
Journal	Journal of Biotechnology
Volume	17
Issue number	1
DOIs	https://doi.org/10.1016/0168-1656(91)90025-Q
Publication status	Published - 1 Jan 1991
MoE publication type	A1 Journal article-refereed

Keywords

Cellulase
Chymosin
Filamentous fungi
Heterologous gene expression
Trichoderma reesei

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10.1016/0168-1656(91)90025-Q

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title = "Enzyme production by recombinant Trichoderma reesei strains",

abstract = "The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.",

keywords = "Cellulase, Chymosin, Filamentous fungi, Heterologous gene expression, Trichoderma reesei",

author = "Uusitalo, {Jaana M.} and {Helena Nevalainen}, {K. M.} and Harkki, {Anu M.} and Knowles, {Jonathan K.C.} and Penttil{\"a}, {Merja E.}",

year = "1991",

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doi = "10.1016/0168-1656(91)90025-Q",

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TY - JOUR

T1 - Enzyme production by recombinant Trichoderma reesei strains

AU - Uusitalo, Jaana M.

AU - Helena Nevalainen, K. M.

AU - Harkki, Anu M.

AU - Knowles, Jonathan K.C.

AU - Penttilä, Merja E.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

AB - The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

KW - Cellulase

KW - Chymosin

KW - Filamentous fungi

KW - Heterologous gene expression

KW - Trichoderma reesei

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DO - 10.1016/0168-1656(91)90025-Q

M3 - Article

C2 - 1367015

AN - SCOPUS:0025957467

SN - 0168-1656

VL - 17

SP - 35

EP - 49

JO - Journal of Biotechnology

JF - Journal of Biotechnology

IS - 1

ER -

Enzyme production by recombinant Trichoderma reesei strains

Abstract

Keywords

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