Enzyme production by recombinant Trichoderma reesei strains

Jaana M. Uusitalo, K. M. Helena Nevalainen, Anu M. Harkki, Jonathan K.C. Knowles, Merja E. Penttilä

    Research output: Contribution to journalArticleScientificpeer-review

    81 Citations (Scopus)

    Abstract

    The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

    Original languageEnglish
    Pages (from-to)35-49
    Number of pages15
    JournalJournal of Biotechnology
    Volume17
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 1991
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Cellulase
    • Chymosin
    • Filamentous fungi
    • Heterologous gene expression
    • Trichoderma reesei

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