EphB2 expression across 138 human tumor types in a tissue microarray

High levels of expression in gastrointestinal cancers

Alessandro Lugli, Hanspeter Spichtin, Robert Maurer, Martina Mirlacher, Jeff Kiefer, Pia Huusko, David Azorsa, Luigi Terracciano, Guido Sauter, Olli Kallioniemi, Spyro Mousses, Luigi Tornillo (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Purpose: To comprehensively evaluate ephrin receptor B2 (EphB2) expression in normal and neoplastic tissues. EphB2 is a tyrosine kinase recently implicated in the deregulation of cell-to-cell communication in many tumors.

Experimental Design: EphB2 protein expression was analyzed by immunohistochemistry on tissue microarrays that included 76 different normal tissues, >4,000 samples from 138 different cancer types, and 1,476 samples of colon cancer with clinical follow-up data.

Results: We found most prominent EphB2 expression in the intestinal epithelium (colonic crypts) with cancer of the colorectum displaying the highest EphB2 positivity of all tumors. Positivity was found in 100% of 118 colon adenomas but in 33.3% of 45 colon carcinomas. EphB2 expression was also observed in 75 tumor categories, including serous carcinoma of the endometrium (34.8%), adenocarcinoma of the esophagus (33.3%), intestinal adenocarcinoma of the stomach (30.2%), and adenocarcinoma of the small intestine (70%). The occasional finding of strong EphB2 positivity in tumors without EphB2 positivity in the corresponding normal cells [adenocarcinoma of the lung (4%) and pancreas (2.2%)] suggests that deregulation of EphB2 signaling may involve up-regulation of the protein expression. In colon carcinoma, loss of EphB2 expression was associated with advanced stage (P < 0.0001) and was an indicator of poor overall survival (P = 0.0098).

Conclusions: Our results provide an overview on the EphB2 protein expression in normal and neoplastic tissues. Deregulated EphB2 expression may play a role in several cancer types with loss of EphB2 expression serving as an indicator of the possible pathogenetic role of EphB2 signaling in the maintenance of tissue architecture of colon epithelium.

Original languageEnglish
Pages (from-to)6450 - 6458
Number of pages9
JournalClinical Cancer Research
Volume11
Issue number18
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

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Ephrin-B2
Eph Family Receptors
Gastrointestinal Neoplasms
Neoplasms
Colon
Adenocarcinoma
Carcinoma
Proteins

Cite this

Lugli, Alessandro ; Spichtin, Hanspeter ; Maurer, Robert ; Mirlacher, Martina ; Kiefer, Jeff ; Huusko, Pia ; Azorsa, David ; Terracciano, Luigi ; Sauter, Guido ; Kallioniemi, Olli ; Mousses, Spyro ; Tornillo, Luigi. / EphB2 expression across 138 human tumor types in a tissue microarray : High levels of expression in gastrointestinal cancers. In: Clinical Cancer Research. 2005 ; Vol. 11, No. 18. pp. 6450 - 6458.
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title = "EphB2 expression across 138 human tumor types in a tissue microarray: High levels of expression in gastrointestinal cancers",
abstract = "Purpose: To comprehensively evaluate ephrin receptor B2 (EphB2) expression in normal and neoplastic tissues. EphB2 is a tyrosine kinase recently implicated in the deregulation of cell-to-cell communication in many tumors. Experimental Design: EphB2 protein expression was analyzed by immunohistochemistry on tissue microarrays that included 76 different normal tissues, >4,000 samples from 138 different cancer types, and 1,476 samples of colon cancer with clinical follow-up data. Results: We found most prominent EphB2 expression in the intestinal epithelium (colonic crypts) with cancer of the colorectum displaying the highest EphB2 positivity of all tumors. Positivity was found in 100{\%} of 118 colon adenomas but in 33.3{\%} of 45 colon carcinomas. EphB2 expression was also observed in 75 tumor categories, including serous carcinoma of the endometrium (34.8{\%}), adenocarcinoma of the esophagus (33.3{\%}), intestinal adenocarcinoma of the stomach (30.2{\%}), and adenocarcinoma of the small intestine (70{\%}). The occasional finding of strong EphB2 positivity in tumors without EphB2 positivity in the corresponding normal cells [adenocarcinoma of the lung (4{\%}) and pancreas (2.2{\%})] suggests that deregulation of EphB2 signaling may involve up-regulation of the protein expression. In colon carcinoma, loss of EphB2 expression was associated with advanced stage (P < 0.0001) and was an indicator of poor overall survival (P = 0.0098). Conclusions: Our results provide an overview on the EphB2 protein expression in normal and neoplastic tissues. Deregulated EphB2 expression may play a role in several cancer types with loss of EphB2 expression serving as an indicator of the possible pathogenetic role of EphB2 signaling in the maintenance of tissue architecture of colon epithelium.",
author = "Alessandro Lugli and Hanspeter Spichtin and Robert Maurer and Martina Mirlacher and Jeff Kiefer and Pia Huusko and David Azorsa and Luigi Terracciano and Guido Sauter and Olli Kallioniemi and Spyro Mousses and Luigi Tornillo",
year = "2005",
doi = "10.1158/1078-0432.CCR-04-2458",
language = "English",
volume = "11",
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Lugli, A, Spichtin, H, Maurer, R, Mirlacher, M, Kiefer, J, Huusko, P, Azorsa, D, Terracciano, L, Sauter, G, Kallioniemi, O, Mousses, S & Tornillo, L 2005, 'EphB2 expression across 138 human tumor types in a tissue microarray: High levels of expression in gastrointestinal cancers', Clinical Cancer Research, vol. 11, no. 18, pp. 6450 - 6458. https://doi.org/10.1158/1078-0432.CCR-04-2458

EphB2 expression across 138 human tumor types in a tissue microarray : High levels of expression in gastrointestinal cancers. / Lugli, Alessandro; Spichtin, Hanspeter; Maurer, Robert; Mirlacher, Martina; Kiefer, Jeff; Huusko, Pia; Azorsa, David; Terracciano, Luigi; Sauter, Guido; Kallioniemi, Olli; Mousses, Spyro; Tornillo, Luigi (Corresponding Author).

In: Clinical Cancer Research, Vol. 11, No. 18, 2005, p. 6450 - 6458.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - EphB2 expression across 138 human tumor types in a tissue microarray

T2 - High levels of expression in gastrointestinal cancers

AU - Lugli, Alessandro

AU - Spichtin, Hanspeter

AU - Maurer, Robert

AU - Mirlacher, Martina

AU - Kiefer, Jeff

AU - Huusko, Pia

AU - Azorsa, David

AU - Terracciano, Luigi

AU - Sauter, Guido

AU - Kallioniemi, Olli

AU - Mousses, Spyro

AU - Tornillo, Luigi

PY - 2005

Y1 - 2005

N2 - Purpose: To comprehensively evaluate ephrin receptor B2 (EphB2) expression in normal and neoplastic tissues. EphB2 is a tyrosine kinase recently implicated in the deregulation of cell-to-cell communication in many tumors. Experimental Design: EphB2 protein expression was analyzed by immunohistochemistry on tissue microarrays that included 76 different normal tissues, >4,000 samples from 138 different cancer types, and 1,476 samples of colon cancer with clinical follow-up data. Results: We found most prominent EphB2 expression in the intestinal epithelium (colonic crypts) with cancer of the colorectum displaying the highest EphB2 positivity of all tumors. Positivity was found in 100% of 118 colon adenomas but in 33.3% of 45 colon carcinomas. EphB2 expression was also observed in 75 tumor categories, including serous carcinoma of the endometrium (34.8%), adenocarcinoma of the esophagus (33.3%), intestinal adenocarcinoma of the stomach (30.2%), and adenocarcinoma of the small intestine (70%). The occasional finding of strong EphB2 positivity in tumors without EphB2 positivity in the corresponding normal cells [adenocarcinoma of the lung (4%) and pancreas (2.2%)] suggests that deregulation of EphB2 signaling may involve up-regulation of the protein expression. In colon carcinoma, loss of EphB2 expression was associated with advanced stage (P < 0.0001) and was an indicator of poor overall survival (P = 0.0098). Conclusions: Our results provide an overview on the EphB2 protein expression in normal and neoplastic tissues. Deregulated EphB2 expression may play a role in several cancer types with loss of EphB2 expression serving as an indicator of the possible pathogenetic role of EphB2 signaling in the maintenance of tissue architecture of colon epithelium.

AB - Purpose: To comprehensively evaluate ephrin receptor B2 (EphB2) expression in normal and neoplastic tissues. EphB2 is a tyrosine kinase recently implicated in the deregulation of cell-to-cell communication in many tumors. Experimental Design: EphB2 protein expression was analyzed by immunohistochemistry on tissue microarrays that included 76 different normal tissues, >4,000 samples from 138 different cancer types, and 1,476 samples of colon cancer with clinical follow-up data. Results: We found most prominent EphB2 expression in the intestinal epithelium (colonic crypts) with cancer of the colorectum displaying the highest EphB2 positivity of all tumors. Positivity was found in 100% of 118 colon adenomas but in 33.3% of 45 colon carcinomas. EphB2 expression was also observed in 75 tumor categories, including serous carcinoma of the endometrium (34.8%), adenocarcinoma of the esophagus (33.3%), intestinal adenocarcinoma of the stomach (30.2%), and adenocarcinoma of the small intestine (70%). The occasional finding of strong EphB2 positivity in tumors without EphB2 positivity in the corresponding normal cells [adenocarcinoma of the lung (4%) and pancreas (2.2%)] suggests that deregulation of EphB2 signaling may involve up-regulation of the protein expression. In colon carcinoma, loss of EphB2 expression was associated with advanced stage (P < 0.0001) and was an indicator of poor overall survival (P = 0.0098). Conclusions: Our results provide an overview on the EphB2 protein expression in normal and neoplastic tissues. Deregulated EphB2 expression may play a role in several cancer types with loss of EphB2 expression serving as an indicator of the possible pathogenetic role of EphB2 signaling in the maintenance of tissue architecture of colon epithelium.

U2 - 10.1158/1078-0432.CCR-04-2458

DO - 10.1158/1078-0432.CCR-04-2458

M3 - Article

VL - 11

SP - 6450

EP - 6458

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

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ER -