Epifluorescence image analysis and cultivation of foodborne biofilm bacteria grown on stainless steel surfaces

Gun Wirtanen (Corresponding Author), Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

Abstract

In this study, biofilm was grown on stainless steel surfaces (AISI 304) for 2, 5, and 10 d at 25°C in slime broth inoculated with the food spoilage microbes Bacillus subtilis, Listeria monocytogenes, Pediococcus pentosaceus, and Pseudomonas fragi. The biofilm developing on steel surfaces were investigated using conventional plating, quantitative glycocalyx determination, and epifluorescence microscopy with image analysis. The results showed that B. subtilis and P. fragi could easily be cultivated after 2 d growth. After a growth period of 10 d, the cells were difficult to cultivate from the surface, and the growth was detected better by microscopy. L. monocytogenes, on the other hand, could easily be detected by cultivation after 2, 5, and 10 d. The greatest amount of slime was produced by P. pentosaceus, as was also shown by epifluorescence microscopy.

Original languageEnglish
Pages (from-to)678 - 683
Number of pages6
JournalJournal of Food Protection
Volume56
Issue number8
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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