Epifluorescence image analysis and cultivation of foodborne biofilm bacteria grown on stainless steel surfaces

Gun Wirtanen (Corresponding Author), Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

Abstract

In this study, biofilm was grown on stainless steel surfaces (AISI 304) for 2, 5, and 10 d at 25°C in slime broth inoculated with the food spoilage microbes Bacillus subtilis, Listeria monocytogenes, Pediococcus pentosaceus, and Pseudomonas fragi. The biofilm developing on steel surfaces were investigated using conventional plating, quantitative glycocalyx determination, and epifluorescence microscopy with image analysis. The results showed that B. subtilis and P. fragi could easily be cultivated after 2 d growth. After a growth period of 10 d, the cells were difficult to cultivate from the surface, and the growth was detected better by microscopy. L. monocytogenes, on the other hand, could easily be detected by cultivation after 2, 5, and 10 d. The greatest amount of slime was produced by P. pentosaceus, as was also shown by epifluorescence microscopy.

Original languageEnglish
Pages (from-to)678 - 683
Number of pages6
JournalJournal of Food Protection
Volume56
Issue number8
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

Fingerprint

Pseudomonas fragi
Stainless Steel
stainless steel
Biofilms
biofilm
Pediococcus pentosaceus
Microscopy
microscopy
Listeria monocytogenes
image analysis
Bacillus subtilis
Bacteria
bacteria
Growth
Glycocalyx
food spoilage
Steel
steel
microorganisms
Food

Cite this

@article{eb3408f91c304059b28ffe66cf5ddd3a,
title = "Epifluorescence image analysis and cultivation of foodborne biofilm bacteria grown on stainless steel surfaces",
abstract = "In this study, biofilm was grown on stainless steel surfaces (AISI 304) for 2, 5, and 10 d at 25°C in slime broth inoculated with the food spoilage microbes Bacillus subtilis, Listeria monocytogenes, Pediococcus pentosaceus, and Pseudomonas fragi. The biofilm developing on steel surfaces were investigated using conventional plating, quantitative glycocalyx determination, and epifluorescence microscopy with image analysis. The results showed that B. subtilis and P. fragi could easily be cultivated after 2 d growth. After a growth period of 10 d, the cells were difficult to cultivate from the surface, and the growth was detected better by microscopy. L. monocytogenes, on the other hand, could easily be detected by cultivation after 2, 5, and 10 d. The greatest amount of slime was produced by P. pentosaceus, as was also shown by epifluorescence microscopy.",
author = "Gun Wirtanen and Tiina Mattila-Sandholm",
note = "Project code: ELI12002",
year = "1993",
doi = "10.4315/0362-028X-56.8.678",
language = "English",
volume = "56",
pages = "678 -- 683",
journal = "Journal of Food Protection",
issn = "0362-028X",
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Epifluorescence image analysis and cultivation of foodborne biofilm bacteria grown on stainless steel surfaces. / Wirtanen, Gun (Corresponding Author); Mattila-Sandholm, Tiina.

In: Journal of Food Protection, Vol. 56, No. 8, 1993, p. 678 - 683.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Epifluorescence image analysis and cultivation of foodborne biofilm bacteria grown on stainless steel surfaces

AU - Wirtanen, Gun

AU - Mattila-Sandholm, Tiina

N1 - Project code: ELI12002

PY - 1993

Y1 - 1993

N2 - In this study, biofilm was grown on stainless steel surfaces (AISI 304) for 2, 5, and 10 d at 25°C in slime broth inoculated with the food spoilage microbes Bacillus subtilis, Listeria monocytogenes, Pediococcus pentosaceus, and Pseudomonas fragi. The biofilm developing on steel surfaces were investigated using conventional plating, quantitative glycocalyx determination, and epifluorescence microscopy with image analysis. The results showed that B. subtilis and P. fragi could easily be cultivated after 2 d growth. After a growth period of 10 d, the cells were difficult to cultivate from the surface, and the growth was detected better by microscopy. L. monocytogenes, on the other hand, could easily be detected by cultivation after 2, 5, and 10 d. The greatest amount of slime was produced by P. pentosaceus, as was also shown by epifluorescence microscopy.

AB - In this study, biofilm was grown on stainless steel surfaces (AISI 304) for 2, 5, and 10 d at 25°C in slime broth inoculated with the food spoilage microbes Bacillus subtilis, Listeria monocytogenes, Pediococcus pentosaceus, and Pseudomonas fragi. The biofilm developing on steel surfaces were investigated using conventional plating, quantitative glycocalyx determination, and epifluorescence microscopy with image analysis. The results showed that B. subtilis and P. fragi could easily be cultivated after 2 d growth. After a growth period of 10 d, the cells were difficult to cultivate from the surface, and the growth was detected better by microscopy. L. monocytogenes, on the other hand, could easily be detected by cultivation after 2, 5, and 10 d. The greatest amount of slime was produced by P. pentosaceus, as was also shown by epifluorescence microscopy.

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