Estimation of spatial genetic structure in inter-regional populations of Trigonella foenum-graceum L. species through phenotypic variation and seed protein profiling

Jawad Ali Shah, Muhammad Mujtaba, Sohail Ahmad Jan, Sultan Akbar Jadoon, Malik Ashiq Rabbani, Abdul Ghafoor, Muhammad Jahanzaib, Haris Khurshid

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Fenugreek (Trigonella foenum-graceum L.) is an important legume crop mainly grown for its pharmacological and nutritional value in Mediterranean region, western Asia, Indian sub-continent and Africa. We evaluated 110 fenugreek accessions from diverse agro-ecological regions i.e. South Asia, Mediterranean, Middle East, Europe and Africa for phenotypic divergence and seed protein based variation. Significant agro-morphological variability was revealed by germplasm viz-a'-viz traits e.g days to flower initiation, days to flower completion, yield plant-1, plant habit, vigor, flower colour and plant height. Multivariate approach of Principal Component Analysis and Euclidean distance generated dendrogram distributed all accessions into 6 and 9 distinct groups for morpho-agronomic dissimilarities, respectively. Four principal components (PCs) with Eigen value higher than unity (E > 1), represented 65% variability in germplasm. Geographical distribution was evident by scatter plot as germplasm figured in 6 different sub-populations. Iranian accessions were most diverse, showing up in all sub-populations followed by Indian, Turkish, Ethiopian, Pakistani and Egyptian accessions which ranked in 5,4,4,3 and 3 sub-populations, respectively. Electrophoretic pattern of seed protein also exhibited considerable polymorphism in the range of 30~100 kDa. Maximum of 16 bands were produced in Turkish PI171872 and Indian PI175321 genotypes. UPGMA based cluster analysis distributed all accessions in 5 groups where accessions from close geographical proximity settled adjacently.

Original languageEnglish
Pages (from-to)171-185
JournalGenetika
Volume50
Issue number1
DOIs
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed

Keywords

  • Fenugreek
  • Genetic diversity
  • GIS
  • Spatial heterogeneity

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