Ethanol production from wood via concentrated acid hydrolysis, chromatographic separation, and fermentation

Jari Heinonen, Anu Tamminen, Jaana Uusitalo, Tuomo Sainio (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

BACKGROUND: Production of bioethanol from wood using concentrated acid hydrolysis has received less attention than the dilute acid hydrolysis route. The feasibility of producing lignocellulosic bioethanol from spruce and birch via concentrated acid hydrolysis was studied experimentally. Hydrolysis with sulfuric acid, chromatographic purification of the hydrolysate, and fermentation of the monosaccharides were investigated.

RESULTS: Monosaccharide yields of 70% were obtained in the hydrolysis of spruce and birch. Only low amounts of by‐products were formed. With chromatographic purification of the hydrolysate, over 90% of the hydrolysis acid was recovered for recycling, and furfural and HMF were removed completely. Most of the acetic acid was recovered in a separate fraction. The monosaccharide yield in a single pass separation was approximately 70%. In the fermentation, S. cerevisiae produced higher amounts of ethanol and more efficiently than P. stipitis. Chromatographically purified hydrolysates gave higher ethanol productivities and yields than Ca(OH)2 neutralized hydrolysates.

CONCLUSIONS: Chromatographic purification of concentrated acid lignocellulosic hydrolysates has advantages when compared with neutralization with Ca(OH)2. With chromatography, most of the inhibitory compounds can be removed from the hydrolysates. In addition, due to the recycling of the hydrolysis acid, the economy of the bioethanol manufacturing process is increased considerably.
Original languageEnglish
Pages (from-to)689-696
Number of pages8
JournalJournal of Chemical Technology and Biotechnology
Volume87
Issue number5
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Fermentation
fermentation
ethanol
hydrolysis
Hydrolysis
Wood
Ethanol
Acids
acid
Bioethanol
monosaccharide
Monosaccharides
Purification
purification
Betula
Recycling
recycling
Furaldehyde
Furfural
neutralization

Keywords

  • lignocellulose
  • bioethanol
  • concentrated acid hydrolysis
  • chromatographic separation
  • detoxification
  • fermentation

Cite this

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title = "Ethanol production from wood via concentrated acid hydrolysis, chromatographic separation, and fermentation",
abstract = "BACKGROUND: Production of bioethanol from wood using concentrated acid hydrolysis has received less attention than the dilute acid hydrolysis route. The feasibility of producing lignocellulosic bioethanol from spruce and birch via concentrated acid hydrolysis was studied experimentally. Hydrolysis with sulfuric acid, chromatographic purification of the hydrolysate, and fermentation of the monosaccharides were investigated. RESULTS: Monosaccharide yields of 70{\%} were obtained in the hydrolysis of spruce and birch. Only low amounts of by‐products were formed. With chromatographic purification of the hydrolysate, over 90{\%} of the hydrolysis acid was recovered for recycling, and furfural and HMF were removed completely. Most of the acetic acid was recovered in a separate fraction. The monosaccharide yield in a single pass separation was approximately 70{\%}. In the fermentation, S. cerevisiae produced higher amounts of ethanol and more efficiently than P. stipitis. Chromatographically purified hydrolysates gave higher ethanol productivities and yields than Ca(OH)2 neutralized hydrolysates. CONCLUSIONS: Chromatographic purification of concentrated acid lignocellulosic hydrolysates has advantages when compared with neutralization with Ca(OH)2. With chromatography, most of the inhibitory compounds can be removed from the hydrolysates. In addition, due to the recycling of the hydrolysis acid, the economy of the bioethanol manufacturing process is increased considerably.",
keywords = "lignocellulose, bioethanol, concentrated acid hydrolysis, chromatographic separation, detoxification, fermentation",
author = "Jari Heinonen and Anu Tamminen and Jaana Uusitalo and Tuomo Sainio",
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Ethanol production from wood via concentrated acid hydrolysis, chromatographic separation, and fermentation. / Heinonen, Jari; Tamminen, Anu; Uusitalo, Jaana; Sainio, Tuomo (Corresponding Author).

In: Journal of Chemical Technology and Biotechnology, Vol. 87, No. 5, 2012, p. 689-696.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Ethanol production from wood via concentrated acid hydrolysis, chromatographic separation, and fermentation

AU - Heinonen, Jari

AU - Tamminen, Anu

AU - Uusitalo, Jaana

AU - Sainio, Tuomo

PY - 2012

Y1 - 2012

N2 - BACKGROUND: Production of bioethanol from wood using concentrated acid hydrolysis has received less attention than the dilute acid hydrolysis route. The feasibility of producing lignocellulosic bioethanol from spruce and birch via concentrated acid hydrolysis was studied experimentally. Hydrolysis with sulfuric acid, chromatographic purification of the hydrolysate, and fermentation of the monosaccharides were investigated. RESULTS: Monosaccharide yields of 70% were obtained in the hydrolysis of spruce and birch. Only low amounts of by‐products were formed. With chromatographic purification of the hydrolysate, over 90% of the hydrolysis acid was recovered for recycling, and furfural and HMF were removed completely. Most of the acetic acid was recovered in a separate fraction. The monosaccharide yield in a single pass separation was approximately 70%. In the fermentation, S. cerevisiae produced higher amounts of ethanol and more efficiently than P. stipitis. Chromatographically purified hydrolysates gave higher ethanol productivities and yields than Ca(OH)2 neutralized hydrolysates. CONCLUSIONS: Chromatographic purification of concentrated acid lignocellulosic hydrolysates has advantages when compared with neutralization with Ca(OH)2. With chromatography, most of the inhibitory compounds can be removed from the hydrolysates. In addition, due to the recycling of the hydrolysis acid, the economy of the bioethanol manufacturing process is increased considerably.

AB - BACKGROUND: Production of bioethanol from wood using concentrated acid hydrolysis has received less attention than the dilute acid hydrolysis route. The feasibility of producing lignocellulosic bioethanol from spruce and birch via concentrated acid hydrolysis was studied experimentally. Hydrolysis with sulfuric acid, chromatographic purification of the hydrolysate, and fermentation of the monosaccharides were investigated. RESULTS: Monosaccharide yields of 70% were obtained in the hydrolysis of spruce and birch. Only low amounts of by‐products were formed. With chromatographic purification of the hydrolysate, over 90% of the hydrolysis acid was recovered for recycling, and furfural and HMF were removed completely. Most of the acetic acid was recovered in a separate fraction. The monosaccharide yield in a single pass separation was approximately 70%. In the fermentation, S. cerevisiae produced higher amounts of ethanol and more efficiently than P. stipitis. Chromatographically purified hydrolysates gave higher ethanol productivities and yields than Ca(OH)2 neutralized hydrolysates. CONCLUSIONS: Chromatographic purification of concentrated acid lignocellulosic hydrolysates has advantages when compared with neutralization with Ca(OH)2. With chromatography, most of the inhibitory compounds can be removed from the hydrolysates. In addition, due to the recycling of the hydrolysis acid, the economy of the bioethanol manufacturing process is increased considerably.

KW - lignocellulose

KW - bioethanol

KW - concentrated acid hydrolysis

KW - chromatographic separation

KW - detoxification

KW - fermentation

U2 - 10.1002/jctb.2766

DO - 10.1002/jctb.2766

M3 - Article

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SP - 689

EP - 696

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 5

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