Evaluating the biodegradation of aromatic hydrocarbons by monitoring of several functional genes

Reetta Piskonen (Corresponding Author), Mari Nyyssönen, Merja Itävaara

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Various microbial activities determine the effectiveness of bioremediation processes. In this work, we evaluated the feasibility of gene array hybridization for monitoring the efficiency of biodegradation processes. Biodegradation of 14C-labelled naphthalene and toluene by the aromatic hydrocarbon-degrading Pseudomonas putida F1, P. putida mt-2 and P. putida G7 was followed in mixed liquid culture microcosm by a preliminary, nylon membrane-based gene array. In the beginning of the study, toluene was degraded rapidly and increased amount of toluene degradation genes was detected by the preliminary gene array developed for the study. After toluene was degraded, naphthalene mineralization started and the amount of naphthalene degradation genes increased as biodegradation proceeded. The amount of toluene degradation genes decreased towards the end of the study. The hybridization signal intensities determined by preliminary gene array were in good agreement with mineralization of naphthalene and toluene and with the amount of naphthalene dioxygenase and toluene dioxygenase genes quantified by dot blot hybridization. The clear correlation between the results obtained by the preliminary array and the biodegradation process suggests that gene array methods can be considered as a promising tool for monitoring the efficiency of biodegradation processes.
Original languageEnglish
Pages (from-to)883-895
Number of pages13
JournalBiodegradation
Volume19
Issue number6
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Aromatic Hydrocarbons
Aromatic hydrocarbons
Biodegradation
aromatic hydrocarbon
biodegradation
Genes
toluene
Toluene
Naphthalene
Monitoring
gene
naphthalene
monitoring
Pseudomonas putida
Degradation
degradation
mineralization
Environmental Biodegradation
Bioremediation
Nylons

Keywords

  • aromatic hydrocarbons
  • biodegradation
  • functional gene
  • monitoring
  • process

Cite this

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title = "Evaluating the biodegradation of aromatic hydrocarbons by monitoring of several functional genes",
abstract = "Various microbial activities determine the effectiveness of bioremediation processes. In this work, we evaluated the feasibility of gene array hybridization for monitoring the efficiency of biodegradation processes. Biodegradation of 14C-labelled naphthalene and toluene by the aromatic hydrocarbon-degrading Pseudomonas putida F1, P. putida mt-2 and P. putida G7 was followed in mixed liquid culture microcosm by a preliminary, nylon membrane-based gene array. In the beginning of the study, toluene was degraded rapidly and increased amount of toluene degradation genes was detected by the preliminary gene array developed for the study. After toluene was degraded, naphthalene mineralization started and the amount of naphthalene degradation genes increased as biodegradation proceeded. The amount of toluene degradation genes decreased towards the end of the study. The hybridization signal intensities determined by preliminary gene array were in good agreement with mineralization of naphthalene and toluene and with the amount of naphthalene dioxygenase and toluene dioxygenase genes quantified by dot blot hybridization. The clear correlation between the results obtained by the preliminary array and the biodegradation process suggests that gene array methods can be considered as a promising tool for monitoring the efficiency of biodegradation processes.",
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Evaluating the biodegradation of aromatic hydrocarbons by monitoring of several functional genes. / Piskonen, Reetta (Corresponding Author); Nyyssönen, Mari; Itävaara, Merja.

In: Biodegradation, Vol. 19, No. 6, 2008, p. 883-895.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Nyyssönen, Mari

AU - Itävaara, Merja

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