Evaluation of epifluorescence image analysis of biofilm growth on stainless steel surfaces

Gun Wirtanen (Corresponding Author), Timo Alanko, Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

Biofilm growth of Bacillus subtilis, Pseudomonas fragi, Pediococcus inopinatus and Listeria monocytogenes was studied on stainless steel surfaces at room and low temperatures to evaluate the results of traditional hygiene measures. The results were compared with those of image analysis of stainless steel surfaces in an epifluorescence microscope. Statistical analyses were carried out to determine the variations between the conventional cultivation swab method, the glycocalyx amount obtained using swabbing, and the values of the areas of the biofilm, slime and cells. As a general rule, old biofilms showed total counts at approximately the same levels as the young biofilm. The results showed that temperature affected the results for all strains except B. subtilis. The strains of Pe. inopinatus and Ps. fragi showed increased attachment at 6°C and L. monocytogenes at 25°C. The biofilm slime was more easily detached than the cells. The results indicated that the traditional swab method is not reliable for the measurement of biofilm formation on surfaces.
Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume5
Issue number6
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

Fingerprint

biofilms
Stainless Steel
Biofilms
image analysis
Image analysis
stainless steels
Stainless steel
evaluation
Pseudomonas fragi
Growth
Listeria monocytogenes
Bacillus subtilis
hygiene
Pediococcus
Listeria
Glycocalyx
Temperature
pseudomonas
Bacillus
Bacilli

Cite this

Wirtanen, Gun ; Alanko, Timo ; Mattila-Sandholm, Tiina. / Evaluation of epifluorescence image analysis of biofilm growth on stainless steel surfaces. In: Colloids and Surfaces B: Biointerfaces. 1996 ; Vol. 5, No. 6. pp. 319-326.
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abstract = "Biofilm growth of Bacillus subtilis, Pseudomonas fragi, Pediococcus inopinatus and Listeria monocytogenes was studied on stainless steel surfaces at room and low temperatures to evaluate the results of traditional hygiene measures. The results were compared with those of image analysis of stainless steel surfaces in an epifluorescence microscope. Statistical analyses were carried out to determine the variations between the conventional cultivation swab method, the glycocalyx amount obtained using swabbing, and the values of the areas of the biofilm, slime and cells. As a general rule, old biofilms showed total counts at approximately the same levels as the young biofilm. The results showed that temperature affected the results for all strains except B. subtilis. The strains of Pe. inopinatus and Ps. fragi showed increased attachment at 6°C and L. monocytogenes at 25°C. The biofilm slime was more easily detached than the cells. The results indicated that the traditional swab method is not reliable for the measurement of biofilm formation on surfaces.",
author = "Gun Wirtanen and Timo Alanko and Tiina Mattila-Sandholm",
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Evaluation of epifluorescence image analysis of biofilm growth on stainless steel surfaces. / Wirtanen, Gun (Corresponding Author); Alanko, Timo; Mattila-Sandholm, Tiina.

In: Colloids and Surfaces B: Biointerfaces, Vol. 5, No. 6, 1996, p. 319-326.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Evaluation of epifluorescence image analysis of biofilm growth on stainless steel surfaces

AU - Wirtanen, Gun

AU - Alanko, Timo

AU - Mattila-Sandholm, Tiina

N1 - Project code: B5SU00115

PY - 1996

Y1 - 1996

N2 - Biofilm growth of Bacillus subtilis, Pseudomonas fragi, Pediococcus inopinatus and Listeria monocytogenes was studied on stainless steel surfaces at room and low temperatures to evaluate the results of traditional hygiene measures. The results were compared with those of image analysis of stainless steel surfaces in an epifluorescence microscope. Statistical analyses were carried out to determine the variations between the conventional cultivation swab method, the glycocalyx amount obtained using swabbing, and the values of the areas of the biofilm, slime and cells. As a general rule, old biofilms showed total counts at approximately the same levels as the young biofilm. The results showed that temperature affected the results for all strains except B. subtilis. The strains of Pe. inopinatus and Ps. fragi showed increased attachment at 6°C and L. monocytogenes at 25°C. The biofilm slime was more easily detached than the cells. The results indicated that the traditional swab method is not reliable for the measurement of biofilm formation on surfaces.

AB - Biofilm growth of Bacillus subtilis, Pseudomonas fragi, Pediococcus inopinatus and Listeria monocytogenes was studied on stainless steel surfaces at room and low temperatures to evaluate the results of traditional hygiene measures. The results were compared with those of image analysis of stainless steel surfaces in an epifluorescence microscope. Statistical analyses were carried out to determine the variations between the conventional cultivation swab method, the glycocalyx amount obtained using swabbing, and the values of the areas of the biofilm, slime and cells. As a general rule, old biofilms showed total counts at approximately the same levels as the young biofilm. The results showed that temperature affected the results for all strains except B. subtilis. The strains of Pe. inopinatus and Ps. fragi showed increased attachment at 6°C and L. monocytogenes at 25°C. The biofilm slime was more easily detached than the cells. The results indicated that the traditional swab method is not reliable for the measurement of biofilm formation on surfaces.

U2 - 10.1016/0927-7765(95)01226-5

DO - 10.1016/0927-7765(95)01226-5

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EP - 326

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 6

ER -