Evaluation of the role of xyloglucanase in the enzymatic hydrolysis of lignocellulosic substrates

Zsuzsa Benkõ (Corresponding Author), Matti Siika-aho, Liisa Viikari, Kati Réczey

Research output: Contribution to journalArticleScientificpeer-review

47 Citations (Scopus)

Abstract

Xyloglucan is one of the major components in the primary cell wall of higher plants. It is intimately associated with cellulose chains, forming the load-bearing network of the cell wall. Enzymatic degradation of the xyloglucan polymer by xyloglucanases could potentially improve the overall hydrolysis of lignocellulosic substrates by enabling cellulases to hydrolyze the cellulose polymer more efficiently due to the increased surface area. In order to evaluate the role of xyloglucan in the enzymatic hydrolysis of lignocelluloses, 11 different pretreated cellulosic substrates were hydrolyzed using different combinations of purified cellobiohydrolases I and II, endoglucanase II, xyloglucanases of Trichoderma reesei and Aspergillus β-glucosidase. Xyloglucanase activity improved the total hydrolysis of lignocellulosic substrates in general. These results indicate that this enzyme can be used to enhance total hydrolysis, especially in cases when pretreatment of the lignocellulosic substrate is not optimal.
Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalEnzyme and Microbial Technology
Volume43
Issue number2
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Enzymatic hydrolysis
Hydrolysis
Cellulose 1,4-beta-Cellobiosidase
Substrates
Cellulose
Cell Wall
Polymers
Bearings (structural)
Cells
Glucosidases
Cellulases
Trichoderma
Aspergillus
Weight-Bearing
Enzymes
Degradation
xyloglucan endo(1-4)-beta-D-glucanase
xyloglucan

Keywords

  • Enzymatic saccharification
  • Cellulases
  • Xyloglucanase
  • Lignocellulases
  • Trichoderma reesei

Cite this

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title = "Evaluation of the role of xyloglucanase in the enzymatic hydrolysis of lignocellulosic substrates",
abstract = "Xyloglucan is one of the major components in the primary cell wall of higher plants. It is intimately associated with cellulose chains, forming the load-bearing network of the cell wall. Enzymatic degradation of the xyloglucan polymer by xyloglucanases could potentially improve the overall hydrolysis of lignocellulosic substrates by enabling cellulases to hydrolyze the cellulose polymer more efficiently due to the increased surface area. In order to evaluate the role of xyloglucan in the enzymatic hydrolysis of lignocelluloses, 11 different pretreated cellulosic substrates were hydrolyzed using different combinations of purified cellobiohydrolases I and II, endoglucanase II, xyloglucanases of Trichoderma reesei and Aspergillus β-glucosidase. Xyloglucanase activity improved the total hydrolysis of lignocellulosic substrates in general. These results indicate that this enzyme can be used to enhance total hydrolysis, especially in cases when pretreatment of the lignocellulosic substrate is not optimal.",
keywords = "Enzymatic saccharification, Cellulases, Xyloglucanase, Lignocellulases, Trichoderma reesei",
author = "Zsuzsa Benk{\~o} and Matti Siika-aho and Liisa Viikari and Kati R{\'e}czey",
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language = "English",
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Evaluation of the role of xyloglucanase in the enzymatic hydrolysis of lignocellulosic substrates. / Benkõ, Zsuzsa (Corresponding Author); Siika-aho, Matti; Viikari, Liisa; Réczey, Kati.

In: Enzyme and Microbial Technology, Vol. 43, No. 2, 2008, p. 109-114.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Evaluation of the role of xyloglucanase in the enzymatic hydrolysis of lignocellulosic substrates

AU - Benkõ, Zsuzsa

AU - Siika-aho, Matti

AU - Viikari, Liisa

AU - Réczey, Kati

PY - 2008

Y1 - 2008

N2 - Xyloglucan is one of the major components in the primary cell wall of higher plants. It is intimately associated with cellulose chains, forming the load-bearing network of the cell wall. Enzymatic degradation of the xyloglucan polymer by xyloglucanases could potentially improve the overall hydrolysis of lignocellulosic substrates by enabling cellulases to hydrolyze the cellulose polymer more efficiently due to the increased surface area. In order to evaluate the role of xyloglucan in the enzymatic hydrolysis of lignocelluloses, 11 different pretreated cellulosic substrates were hydrolyzed using different combinations of purified cellobiohydrolases I and II, endoglucanase II, xyloglucanases of Trichoderma reesei and Aspergillus β-glucosidase. Xyloglucanase activity improved the total hydrolysis of lignocellulosic substrates in general. These results indicate that this enzyme can be used to enhance total hydrolysis, especially in cases when pretreatment of the lignocellulosic substrate is not optimal.

AB - Xyloglucan is one of the major components in the primary cell wall of higher plants. It is intimately associated with cellulose chains, forming the load-bearing network of the cell wall. Enzymatic degradation of the xyloglucan polymer by xyloglucanases could potentially improve the overall hydrolysis of lignocellulosic substrates by enabling cellulases to hydrolyze the cellulose polymer more efficiently due to the increased surface area. In order to evaluate the role of xyloglucan in the enzymatic hydrolysis of lignocelluloses, 11 different pretreated cellulosic substrates were hydrolyzed using different combinations of purified cellobiohydrolases I and II, endoglucanase II, xyloglucanases of Trichoderma reesei and Aspergillus β-glucosidase. Xyloglucanase activity improved the total hydrolysis of lignocellulosic substrates in general. These results indicate that this enzyme can be used to enhance total hydrolysis, especially in cases when pretreatment of the lignocellulosic substrate is not optimal.

KW - Enzymatic saccharification

KW - Cellulases

KW - Xyloglucanase

KW - Lignocellulases

KW - Trichoderma reesei

U2 - 10.1016/j.enzmictec.2008.03.005

DO - 10.1016/j.enzmictec.2008.03.005

M3 - Article

VL - 43

SP - 109

EP - 114

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

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