Evaluation of the role of xyloglucanase in the enzymatic hydrolysis of lignocellulosic substrates

Zsuzsa Benkõ (Corresponding Author), Matti Siika-aho, Liisa Viikari, Kati Réczey

    Research output: Contribution to journalArticleScientificpeer-review

    61 Citations (Scopus)


    Xyloglucan is one of the major components in the primary cell wall of higher plants. It is intimately associated with cellulose chains, forming the load-bearing network of the cell wall. Enzymatic degradation of the xyloglucan polymer by xyloglucanases could potentially improve the overall hydrolysis of lignocellulosic substrates by enabling cellulases to hydrolyze the cellulose polymer more efficiently due to the increased surface area. In order to evaluate the role of xyloglucan in the enzymatic hydrolysis of lignocelluloses, 11 different pretreated cellulosic substrates were hydrolyzed using different combinations of purified cellobiohydrolases I and II, endoglucanase II, xyloglucanases of Trichoderma reesei and Aspergillus β-glucosidase. Xyloglucanase activity improved the total hydrolysis of lignocellulosic substrates in general. These results indicate that this enzyme can be used to enhance total hydrolysis, especially in cases when pretreatment of the lignocellulosic substrate is not optimal.
    Original languageEnglish
    Pages (from-to)109-114
    Number of pages6
    JournalEnzyme and Microbial Technology
    Issue number2
    Publication statusPublished - 2008
    MoE publication typeA1 Journal article-refereed


    • Enzymatic saccharification
    • Cellulases
    • Xyloglucanase
    • Lignocellulases
    • Trichoderma reesei


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