Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood

Hetti Palonen, Anne Belinda Thomsen (Corresponding Author), Maija Tenkanen, Anette S. Schmidt, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

157 Citations (Scopus)


The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58–64% cellulose, 2–16% hemicellulose, and 24–30% lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79% of theoretical, was obtained using a pretreatment of 200°C for 10 min at neutral pH.
Original languageEnglish
Pages (from-to)1-17
JournalApplied Biochemistry and Biotechnology
Issue number1
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed


  • Wet oxidation
  • enzymatic hydrolysis
  • lignocellulose
  • cellulases


Dive into the research topics of 'Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood'. Together they form a unique fingerprint.

Cite this