Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood

Hetti Palonen, A. B. Thomsen (Corresponding Author), Maija Tenkanen, A. S. Schmidt, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

119 Citations (Scopus)

Abstract

The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58–64% cellulose, 2–16% hemicellulose, and 24–30% lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79% of theoretical, was obtained using a pretreatment of 200°C for 10 min at neutral pH.
Original languageEnglish
Pages (from-to)1 - 17
Number of pages17
JournalApplied Biochemistry and Biotechnology
Volume117
Issue number1
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Enzymatic hydrolysis
Softwoods
Hydrolysis
Lignin
Oxidation
Temperature
Cellulase
Abies
Picea
Cellulose
Sugars
Oxygen
Water
Liquids
Substrates
Chemical analysis
Pressure
hemicellulose

Keywords

  • Wet oxidation
  • enzymatic hydrolysis
  • lignocellulose
  • cellulases

Cite this

Palonen, Hetti ; Thomsen, A. B. ; Tenkanen, Maija ; Schmidt, A. S. ; Viikari, Liisa. / Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood. In: Applied Biochemistry and Biotechnology. 2004 ; Vol. 117, No. 1. pp. 1 - 17.
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abstract = "The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58–64{\%} cellulose, 2–16{\%} hemicellulose, and 24–30{\%} lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79{\%} of theoretical, was obtained using a pretreatment of 200°C for 10 min at neutral pH.",
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Palonen, H, Thomsen, AB, Tenkanen, M, Schmidt, AS & Viikari, L 2004, 'Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood', Applied Biochemistry and Biotechnology, vol. 117, no. 1, pp. 1 - 17. https://doi.org/10.1385/ABAB:117:1:01

Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood. / Palonen, Hetti; Thomsen, A. B. (Corresponding Author); Tenkanen, Maija; Schmidt, A. S.; Viikari, Liisa.

In: Applied Biochemistry and Biotechnology, Vol. 117, No. 1, 2004, p. 1 - 17.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood

AU - Palonen, Hetti

AU - Thomsen, A. B.

AU - Tenkanen, Maija

AU - Schmidt, A. S.

AU - Viikari, Liisa

PY - 2004

Y1 - 2004

N2 - The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58–64% cellulose, 2–16% hemicellulose, and 24–30% lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79% of theoretical, was obtained using a pretreatment of 200°C for 10 min at neutral pH.

AB - The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58–64% cellulose, 2–16% hemicellulose, and 24–30% lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79% of theoretical, was obtained using a pretreatment of 200°C for 10 min at neutral pH.

KW - Wet oxidation

KW - enzymatic hydrolysis

KW - lignocellulose

KW - cellulases

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DO - 10.1385/ABAB:117:1:01

M3 - Article

VL - 117

SP - 1

EP - 17

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

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ER -