Evidence for post-mortem m-calpain autolysis in porcine muscle

Luigi Pomponio; Rene Lametsch; Anders H. Karlsson; Leonardo Nanni Costa; Alberto Grossi; Per Ertbjerg

doi:10.1016/j.meatsci.2008.03.019

Evidence for post-mortem m-calpain autolysis in porcine muscle

Luigi Pomponio
, Rene Lametsch
, Anders H. Karlsson
, Leonardo Nanni Costa
, Alberto Grossi
, Per Ertbjerg^*

^*Corresponding author for this work

Not published at VTT

Research output: Contribution to journal › Article › Scientific › peer-review

48 Citations (Scopus)

Abstract

The objective of the present work was to characterize changes in calpain activity in pork post-mortem. Samples from pig M. longissimus dorsi and M. semimembranosus were collected three days post-mortem from 75 animals and analyzed with casein zymography. The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. Use of M. longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. The activity of the autolyzed form of m-calpain was detectable at day 3 and further increased at day 6. The results also showed a decrease in the non-autolyzed m-calpain activity during post-mortem storage. Collectively, these results suggest that m-calpain is active post-mortem in porcine muscles.

Original language	English
Pages (from-to)	761-764
Journal	Meat Science
Volume	80
Issue number	3
DOIs	https://doi.org/10.1016/j.meatsci.2008.03.019
Publication status	Published - 2008
MoE publication type	A1 Journal article-refereed

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10.1016/j.meatsci.2008.03.019

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@article{a89c87007e904a33b57594a97303b46a,

title = "Evidence for post-mortem m-calpain autolysis in porcine muscle",

abstract = "The objective of the present work was to characterize changes in calpain activity in pork post-mortem. Samples from pig M. longissimus dorsi and M. semimembranosus were collected three days post-mortem from 75 animals and analyzed with casein zymography. The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. Use of M. longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. The activity of the autolyzed form of m-calpain was detectable at day 3 and further increased at day 6. The results also showed a decrease in the non-autolyzed m-calpain activity during post-mortem storage. Collectively, these results suggest that m-calpain is active post-mortem in porcine muscles.",

author = "Luigi Pomponio and Rene Lametsch and Karlsson, \{Anders H.\} and Costa, \{Leonardo Nanni\} and Alberto Grossi and Per Ertbjerg",

year = "2008",

doi = "10.1016/j.meatsci.2008.03.019",

language = "English",

volume = "80",

pages = "761--764",

journal = "Meat Science",

issn = "0309-1740",

publisher = "Elsevier",

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}

TY - JOUR

T1 - Evidence for post-mortem m-calpain autolysis in porcine muscle

AU - Pomponio, Luigi

AU - Lametsch, Rene

AU - Karlsson, Anders H.

AU - Costa, Leonardo Nanni

AU - Grossi, Alberto

AU - Ertbjerg, Per

PY - 2008

Y1 - 2008

N2 - The objective of the present work was to characterize changes in calpain activity in pork post-mortem. Samples from pig M. longissimus dorsi and M. semimembranosus were collected three days post-mortem from 75 animals and analyzed with casein zymography. The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. Use of M. longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. The activity of the autolyzed form of m-calpain was detectable at day 3 and further increased at day 6. The results also showed a decrease in the non-autolyzed m-calpain activity during post-mortem storage. Collectively, these results suggest that m-calpain is active post-mortem in porcine muscles.

AB - The objective of the present work was to characterize changes in calpain activity in pork post-mortem. Samples from pig M. longissimus dorsi and M. semimembranosus were collected three days post-mortem from 75 animals and analyzed with casein zymography. The results indicated post-mortem autolysis of m-calpain as two m-calpain bands were observed on the zymogram gel. Use of M. longissimus dorsi from three pigs collected at different times during storage further confirmed post-mortem autolysis of m-calpain. The activity of the autolyzed form of m-calpain was detectable at day 3 and further increased at day 6. The results also showed a decrease in the non-autolyzed m-calpain activity during post-mortem storage. Collectively, these results suggest that m-calpain is active post-mortem in porcine muscles.

U2 - 10.1016/j.meatsci.2008.03.019

DO - 10.1016/j.meatsci.2008.03.019

M3 - Article

SN - 0309-1740

VL - 80

SP - 761

EP - 764

JO - Meat Science

JF - Meat Science

IS - 3

ER -

Evidence for post-mortem m-calpain autolysis in porcine muscle

Abstract

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