Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

Peter Richard; Mervi H. Toivari; Merja Penttilä

doi:10.1016/S0014-5793(99)01016-9

Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

Peter Richard, Mervi H. Toivari, Merja Penttilä

Research output: Contribution to journal › Article › Scientific › peer-review

27 Citations (Scopus)

Abstract

The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The following Michaelis constants were estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0). Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in wild type S. cerevisiae cells. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	135-138
Number of pages	4
Journal	FEBS Letters
Volume	457
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)01016-9
Publication status	Published - 20 Aug 1999
MoE publication type	A1 Journal article-refereed

Keywords

D-Xylulose
Enzyme kinetics
Saccharomyces cerevisiae
Xylitol dehydrogenase
Xylose metabolism

Access to Document

10.1016/S0014-5793(99)01016-9

Cite this

@article{8193e12aec7e4677bab7109dff3dc989,

title = "Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase",

abstract = "The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The following Michaelis constants were estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0). Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in wild type S. cerevisiae cells. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "D-Xylulose, Enzyme kinetics, Saccharomyces cerevisiae, Xylitol dehydrogenase, Xylose metabolism",

author = "Peter Richard and Toivari, {Mervi H.} and Merja Penttil{\"a}",

year = "1999",

month = aug,

day = "20",

doi = "10.1016/S0014-5793(99)01016-9",

language = "English",

volume = "457",

pages = "135--138",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "1",

}

TY - JOUR

T1 - Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

AU - Richard, Peter

AU - Toivari, Mervi H.

AU - Penttilä, Merja

PY - 1999/8/20

Y1 - 1999/8/20

N2 - The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The following Michaelis constants were estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0). Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in wild type S. cerevisiae cells. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The following Michaelis constants were estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0). Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in wild type S. cerevisiae cells. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - D-Xylulose

KW - Enzyme kinetics

KW - Saccharomyces cerevisiae

KW - Xylitol dehydrogenase

KW - Xylose metabolism

UR - http://www.scopus.com/inward/record.url?scp=0032769768&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)01016-9

DO - 10.1016/S0014-5793(99)01016-9

M3 - Article

C2 - 10486580

AN - SCOPUS:0032769768

SN - 0014-5793

VL - 457

SP - 135

EP - 138

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this