Experiences of kraft lignin functionalization by enzymatic and chemical oxidation

Anna Kalliola (Corresponding Author), Martta Asikainen, Riku Talja, Tarja Tamminen

    Research output: Contribution to journalArticleScientificpeer-review

    14 Citations (Scopus)


    Linear hydrophilic derivatives are expected to soften lignin and improve its utilization in composite applications. Oxidation by means of laccase in the presence of oxygen was employed in an attempt to functionalize commercial kraft lignin by vanillic acid-PEG ester and ether derivatives. Thielavia arenaria and Melanocarpus albomyces laccases at pH 6 and 8 were used. According to O2 consumption and the increase in molar mass, the tested laccases were active toward the lignin and the vanillic acid derivatives and also formed corresponding phenoxyl radicals. However, homogenous polymerization instead of cross-coupling and functionalization took place. As an alternative, lignin functionalization by the ester derivative by chemical oxidation under alkali-O2 conditions was also tested. Efficient lignin polymerization was observed. However, functionalization was not detected. Interestingly, a clear decrease in lignin glass transition temperature was obtained by an isolation procedure that included freeze-drying. This suggests that functionalization may not be necessary to induce the desired softening effect.
    Original languageEnglish
    Pages (from-to)7336-7351
    Issue number4
    Publication statusPublished - 2014
    MoE publication typeA1 Journal article-refereed


    • kraft lignin
    • vanillic acid
    • polyethylene glycol
    • composite
    • glass transition temperature
    • laccase
    • oxygen
    • oxidation


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