Exploring the metabolic stability of engineered hairy roots after 16 years maintenance

Suvi T. Häkkinen, Elisabeth Moyano, Rosa M. Cusidó, Kirsi-Marja Oksman-Caldentey (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds - commercial examples include compounds such as paclitaxel. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells. Although genetic and metabolic stability has often been connected to transgenic hairy roots, there are only few reports on how a very long-term subculturing effects on the production capacity of hairy roots. In this study, hairy roots producing high tropane alkaloid levels were subjected to 16-year follow-up in relation to genetic and metabolic stability. Cryopreservation method for hairy roots of Hyoscyamus muticus was developed to replace laborious subculturing, and although the post-thaw recovery rates remained low, the expression of transgene remained unaltered in cryopreserved roots. It was shown that although displaying some fluctuation in the metabolite yields, even an exceedingly long-term subculturing was successfully applied without significant loss of metabolic activity.
Original languageEnglish
Article number1486
JournalFrontiers in Plant Science
Volume3
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

secondary metabolites
cell culture
Hyoscyamus muticus
tropane alkaloids
paclitaxel
Rhizobium rhizogenes
new drugs
plant hormones
cryopreservation
cultured cells
transgenes
long term effects
biochemical pathways
production technology
genetically modified organisms
metabolites
neoplasms
infection
cells
methodology

Keywords

  • plant cell culture
  • hairy roots
  • hyoscyamine 6β-hydroxylase
  • scopolamine
  • stability
  • cryopreservation

Cite this

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title = "Exploring the metabolic stability of engineered hairy roots after 16 years maintenance",
abstract = "Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds - commercial examples include compounds such as paclitaxel. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells. Although genetic and metabolic stability has often been connected to transgenic hairy roots, there are only few reports on how a very long-term subculturing effects on the production capacity of hairy roots. In this study, hairy roots producing high tropane alkaloid levels were subjected to 16-year follow-up in relation to genetic and metabolic stability. Cryopreservation method for hairy roots of Hyoscyamus muticus was developed to replace laborious subculturing, and although the post-thaw recovery rates remained low, the expression of transgene remained unaltered in cryopreserved roots. It was shown that although displaying some fluctuation in the metabolite yields, even an exceedingly long-term subculturing was successfully applied without significant loss of metabolic activity.",
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author = "H{\"a}kkinen, {Suvi T.} and Elisabeth Moyano and Cusid{\'o}, {Rosa M.} and Kirsi-Marja Oksman-Caldentey",
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Exploring the metabolic stability of engineered hairy roots after 16 years maintenance. / Häkkinen, Suvi T.; Moyano, Elisabeth; Cusidó, Rosa M.; Oksman-Caldentey, Kirsi-Marja (Corresponding Author).

In: Frontiers in Plant Science, Vol. 3, 1486, 2016.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Häkkinen, Suvi T.

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AU - Cusidó, Rosa M.

AU - Oksman-Caldentey, Kirsi-Marja

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