Exploring the mineralization of hydrophobins at a liquid interface

Alexander Schulz, Marco Fioroni, Markus B. Linder, Andreas Nessel, Marco Bocola, Thomas Subkowski, Ulrich Schwaneberg, Alexander Böker (Corresponding Author), Francisco Rodríguez-Ropero (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Hydrophobins, a class of highly surface active amphiphilic proteins, can stabilize various interfaces. When used for emulsion stabilization, it has been recently shown that they are able to induce mineralization, resulting in hollow capsules. We found that not all types of hydrophobins trigger mineralization, and that the morphology of the mineral changes depending on the selected oil. We investigated the formation of hydrophobin films at interfaces by the use of CD spectroscopy. In order to elucidate the structural features that enhance the mineralization property and give a possible explanation for this behavior, we performed MD-simulations of two representative hydrophobins (EAS for class I and HFBII for class II) at a hexane–water interface, in the presence as well as in the absence of ions. Our studies showed that the class II hydrophobin HFBII, which did not induce mineralization, only slightly changes its structure during adsorption at the oil–water interface or upon addition of Ca2+ and HPO42− ions. In contrast to that, the class I hydrophobin EAS changed its conformation to a large extent during the adsorption and interacted strongly with added ions. We revealed that EAS preorganizes the ions at short distances matching the lattice dimensions of hydroxyapatite. The latter finding yields a straightforward explanation for the observed differences in mineralization behavior and allows us to search for other hydrophobins that could assist mineralization, despite their different functions in nature.
Original languageEnglish
Pages (from-to)11343-11352
JournalSoft Matter
Volume8
Issue number44
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Ions
Liquids
liquids
ions
Adsorption
Durapatite
Emulsions
adsorption
Capsules
Minerals
Conformations
capsules
Oils
Stabilization
emulsions
Spectroscopy
hollow
stabilization
oils
actuators

Cite this

Schulz, A., Fioroni, M., Linder, M. B., Nessel, A., Bocola, M., Subkowski, T., ... Rodríguez-Ropero, F. (2012). Exploring the mineralization of hydrophobins at a liquid interface. Soft Matter, 8(44), 11343-11352. https://doi.org/10.1039/c2sm26428e
Schulz, Alexander ; Fioroni, Marco ; Linder, Markus B. ; Nessel, Andreas ; Bocola, Marco ; Subkowski, Thomas ; Schwaneberg, Ulrich ; Böker, Alexander ; Rodríguez-Ropero, Francisco. / Exploring the mineralization of hydrophobins at a liquid interface. In: Soft Matter. 2012 ; Vol. 8, No. 44. pp. 11343-11352.
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abstract = "Hydrophobins, a class of highly surface active amphiphilic proteins, can stabilize various interfaces. When used for emulsion stabilization, it has been recently shown that they are able to induce mineralization, resulting in hollow capsules. We found that not all types of hydrophobins trigger mineralization, and that the morphology of the mineral changes depending on the selected oil. We investigated the formation of hydrophobin films at interfaces by the use of CD spectroscopy. In order to elucidate the structural features that enhance the mineralization property and give a possible explanation for this behavior, we performed MD-simulations of two representative hydrophobins (EAS for class I and HFBII for class II) at a hexane–water interface, in the presence as well as in the absence of ions. Our studies showed that the class II hydrophobin HFBII, which did not induce mineralization, only slightly changes its structure during adsorption at the oil–water interface or upon addition of Ca2+ and HPO42− ions. In contrast to that, the class I hydrophobin EAS changed its conformation to a large extent during the adsorption and interacted strongly with added ions. We revealed that EAS preorganizes the ions at short distances matching the lattice dimensions of hydroxyapatite. The latter finding yields a straightforward explanation for the observed differences in mineralization behavior and allows us to search for other hydrophobins that could assist mineralization, despite their different functions in nature.",
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Schulz, A, Fioroni, M, Linder, MB, Nessel, A, Bocola, M, Subkowski, T, Schwaneberg, U, Böker, A & Rodríguez-Ropero, F 2012, 'Exploring the mineralization of hydrophobins at a liquid interface', Soft Matter, vol. 8, no. 44, pp. 11343-11352. https://doi.org/10.1039/c2sm26428e

Exploring the mineralization of hydrophobins at a liquid interface. / Schulz, Alexander; Fioroni, Marco; Linder, Markus B.; Nessel, Andreas; Bocola, Marco; Subkowski, Thomas; Schwaneberg, Ulrich; Böker, Alexander (Corresponding Author); Rodríguez-Ropero, Francisco (Corresponding Author).

In: Soft Matter, Vol. 8, No. 44, 2012, p. 11343-11352.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Fioroni, Marco

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AU - Böker, Alexander

AU - Rodríguez-Ropero, Francisco

PY - 2012

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N2 - Hydrophobins, a class of highly surface active amphiphilic proteins, can stabilize various interfaces. When used for emulsion stabilization, it has been recently shown that they are able to induce mineralization, resulting in hollow capsules. We found that not all types of hydrophobins trigger mineralization, and that the morphology of the mineral changes depending on the selected oil. We investigated the formation of hydrophobin films at interfaces by the use of CD spectroscopy. In order to elucidate the structural features that enhance the mineralization property and give a possible explanation for this behavior, we performed MD-simulations of two representative hydrophobins (EAS for class I and HFBII for class II) at a hexane–water interface, in the presence as well as in the absence of ions. Our studies showed that the class II hydrophobin HFBII, which did not induce mineralization, only slightly changes its structure during adsorption at the oil–water interface or upon addition of Ca2+ and HPO42− ions. In contrast to that, the class I hydrophobin EAS changed its conformation to a large extent during the adsorption and interacted strongly with added ions. We revealed that EAS preorganizes the ions at short distances matching the lattice dimensions of hydroxyapatite. The latter finding yields a straightforward explanation for the observed differences in mineralization behavior and allows us to search for other hydrophobins that could assist mineralization, despite their different functions in nature.

AB - Hydrophobins, a class of highly surface active amphiphilic proteins, can stabilize various interfaces. When used for emulsion stabilization, it has been recently shown that they are able to induce mineralization, resulting in hollow capsules. We found that not all types of hydrophobins trigger mineralization, and that the morphology of the mineral changes depending on the selected oil. We investigated the formation of hydrophobin films at interfaces by the use of CD spectroscopy. In order to elucidate the structural features that enhance the mineralization property and give a possible explanation for this behavior, we performed MD-simulations of two representative hydrophobins (EAS for class I and HFBII for class II) at a hexane–water interface, in the presence as well as in the absence of ions. Our studies showed that the class II hydrophobin HFBII, which did not induce mineralization, only slightly changes its structure during adsorption at the oil–water interface or upon addition of Ca2+ and HPO42− ions. In contrast to that, the class I hydrophobin EAS changed its conformation to a large extent during the adsorption and interacted strongly with added ions. We revealed that EAS preorganizes the ions at short distances matching the lattice dimensions of hydroxyapatite. The latter finding yields a straightforward explanation for the observed differences in mineralization behavior and allows us to search for other hydrophobins that could assist mineralization, despite their different functions in nature.

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DO - 10.1039/c2sm26428e

M3 - Article

VL - 8

SP - 11343

EP - 11352

JO - Soft Matter

JF - Soft Matter

SN - 1744-683X

IS - 44

ER -

Schulz A, Fioroni M, Linder MB, Nessel A, Bocola M, Subkowski T et al. Exploring the mineralization of hydrophobins at a liquid interface. Soft Matter. 2012;8(44):11343-11352. https://doi.org/10.1039/c2sm26428e