Expression of a fungal cellobiohydrolase in insect cells

Ingemar von Ossowski, Tuula Teeri, Nisse Kalkkinen, Christian Oker-Blom (Corresponding Author)

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13 Citations (Scopus)

Abstract

The gene forTrichoderma reeseicellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced inSpodoptera frugiperdaandTrichoplusia niinsect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate.

Original languageEnglish
Pages (from-to)25-29
JournalBiochemical and Biophysical Research Communications
Volume233
Issue number1
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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