Expression of a fungal cellobiohydrolase in insect cells

Ingemar von Ossowski; Tuula Teeri; Nisse Kalkkinen; Christian Oker-Blom

doi:10.1006/bbrc.1997.6391

Expression of a fungal cellobiohydrolase in insect cells

Ingemar von Ossowski, Tuula Teeri, Nisse Kalkkinen, Christian Oker-Blom (Corresponding Author)

VTT Technical Research Centre of Finland

University of Helsinki

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

Abstract

The gene forTrichoderma reeseicellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced inSpodoptera frugiperdaandTrichoplusia niinsect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate.

Original language	English
Pages (from-to)	25-29
Journal	Biochemical and Biophysical Research Communications
Volume	233
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.6391
Publication status	Published - 1997
MoE publication type	A1 Journal article-refereed

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10.1006/bbrc.1997.6391

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title = "Expression of a fungal cellobiohydrolase in insect cells",

abstract = "The gene forTrichoderma reeseicellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced inSpodoptera frugiperdaandTrichoplusia niinsect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate.",

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AU - von Ossowski, Ingemar

AU - Teeri, Tuula

AU - Kalkkinen, Nisse

AU - Oker-Blom, Christian

PY - 1997

Y1 - 1997

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AB - The gene forTrichoderma reeseicellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced inSpodoptera frugiperdaandTrichoplusia niinsect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate.

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DO - 10.1006/bbrc.1997.6391

M3 - Article

SN - 0006-291X

VL - 233

SP - 25

EP - 29

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Expression of a fungal cellobiohydrolase in insect cells

Abstract

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