Expression of a fungal cellobiohydrolase in insect cells

Ingemar von Ossowski, Tuula Teeri, Nisse Kalkkinen, Christian Oker-Blom (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

The gene forTrichoderma reeseicellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced inSpodoptera frugiperdaandTrichoplusia niinsect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate.

Original languageEnglish
Pages (from-to)25 - 29
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume233
Issue number1
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Cellulose 1,4-beta-Cellobiosidase
Baculoviridae
Chromatography
Hydrophobic and Hydrophilic Interactions
Immunoblotting
Insects
Genes
Molecular Weight
Molecular weight
Monoclonal Antibodies
Substrates
Proteins

Cite this

Ossowski, Ingemar von ; Teeri, Tuula ; Kalkkinen, Nisse ; Oker-Blom, Christian. / Expression of a fungal cellobiohydrolase in insect cells. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 233, No. 1. pp. 25 - 29.
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Expression of a fungal cellobiohydrolase in insect cells. / Ossowski, Ingemar von; Teeri, Tuula; Kalkkinen, Nisse; Oker-Blom, Christian (Corresponding Author).

In: Biochemical and Biophysical Research Communications, Vol. 233, No. 1, 1997, p. 25 - 29.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

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AU - Oker-Blom, Christian

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JO - Biochemical and Biophysical Research Communications

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