Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue

Maria Kolak, Joanna Gertow, Jukka Westerbacka, Scott A. Summers, Jan Liska, Anders Franco-Cereceda, Matej Orešič, Hannele Yki-Järvinen, Per Eriksson, Rachel M. Fisher (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Background:
Inflammation and increased ceramide concentrations characterise adipose tissue of obese women with high liver fat content compared to equally obese women with normal liver fat content. The present study characterises enzymes involved in ceramide metabolism in subcutaneous and intra-abdominal adipose tissue.

Methods:
Pathways leading to increased ceramide concentrations in inflamed versus non-inflamed adipose tissue were investigated by quantifying expression levels of key enzymes involved in ceramide metabolism. Sphingomyelinases (sphingomyelin phosphodiesterases SMPD1-3) were investigated further using immunohistochemistry to establish their location within adipose tissue, and their mRNA expression levels were determined in subcutaneous and intra-abdominal adipose tissue from both non-obese and obese subject.

Results:
Gene expression levels of sphingomyelinases, enzymes that hydrolyse sphingomyelin to ceramide, rather than enzymes involved in de novo ceramide synthesis, were higher in inflamed compared to non-inflamed adipose tissue of obese women (with high and normal liver fat contents respectively). Sphingomyelinases were localised to both macrophages and adipocytes, but also to blood vessels and to extracellular regions surrounding vessels within adipose tissue. Expression levels of SMPD3 mRNA correlated significantly with concentrations of different ceramides and sphingomyelins. In both non-obese and obese subjects SMPD3 mRNA levels were higher in the more inflamed intra-abdominal compared to the subcutaneous adipose tissue depot.

Conclusions:
Generation of ceramides within adipose tissue as a result of sphingomyelinase action may contribute to inflammation in human adipose tissue.
Original languageEnglish
Article number115
JournalLipids in Health and Disease
Volume11
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Intra-Abdominal Fat
Ceramides
Adipose Tissue
Tissue
Sphingomyelin Phosphodiesterase
Enzymes
Sphingomyelins
Liver
Fats
Messenger RNA
Metabolism
Inflammation
Subcutaneous Fat
Adipocytes
Macrophages
Blood Vessels
Blood vessels
Gene expression
Immunohistochemistry
Gene Expression

Cite this

Kolak, M., Gertow, J., Westerbacka, J., Summers, S. A., Liska, J., Franco-Cereceda, A., ... Fisher, R. M. (2012). Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue. Lipids in Health and Disease, 11, [115]. https://doi.org/10.1186/1476-511X-11-115
Kolak, Maria ; Gertow, Joanna ; Westerbacka, Jukka ; Summers, Scott A. ; Liska, Jan ; Franco-Cereceda, Anders ; Orešič, Matej ; Yki-Järvinen, Hannele ; Eriksson, Per ; Fisher, Rachel M. / Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue. In: Lipids in Health and Disease. 2012 ; Vol. 11.
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title = "Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue",
abstract = "Background:Inflammation and increased ceramide concentrations characterise adipose tissue of obese women with high liver fat content compared to equally obese women with normal liver fat content. The present study characterises enzymes involved in ceramide metabolism in subcutaneous and intra-abdominal adipose tissue.Methods:Pathways leading to increased ceramide concentrations in inflamed versus non-inflamed adipose tissue were investigated by quantifying expression levels of key enzymes involved in ceramide metabolism. Sphingomyelinases (sphingomyelin phosphodiesterases SMPD1-3) were investigated further using immunohistochemistry to establish their location within adipose tissue, and their mRNA expression levels were determined in subcutaneous and intra-abdominal adipose tissue from both non-obese and obese subject.Results:Gene expression levels of sphingomyelinases, enzymes that hydrolyse sphingomyelin to ceramide, rather than enzymes involved in de novo ceramide synthesis, were higher in inflamed compared to non-inflamed adipose tissue of obese women (with high and normal liver fat contents respectively). Sphingomyelinases were localised to both macrophages and adipocytes, but also to blood vessels and to extracellular regions surrounding vessels within adipose tissue. Expression levels of SMPD3 mRNA correlated significantly with concentrations of different ceramides and sphingomyelins. In both non-obese and obese subjects SMPD3 mRNA levels were higher in the more inflamed intra-abdominal compared to the subcutaneous adipose tissue depot.Conclusions:Generation of ceramides within adipose tissue as a result of sphingomyelinase action may contribute to inflammation in human adipose tissue.",
author = "Maria Kolak and Joanna Gertow and Jukka Westerbacka and Summers, {Scott A.} and Jan Liska and Anders Franco-Cereceda and Matej Orešič and Hannele Yki-J{\"a}rvinen and Per Eriksson and Fisher, {Rachel M.}",
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doi = "10.1186/1476-511X-11-115",
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Kolak, M, Gertow, J, Westerbacka, J, Summers, SA, Liska, J, Franco-Cereceda, A, Orešič, M, Yki-Järvinen, H, Eriksson, P & Fisher, RM 2012, 'Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue', Lipids in Health and Disease, vol. 11, 115. https://doi.org/10.1186/1476-511X-11-115

Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue. / Kolak, Maria; Gertow, Joanna; Westerbacka, Jukka; Summers, Scott A.; Liska, Jan; Franco-Cereceda, Anders; Orešič, Matej; Yki-Järvinen, Hannele; Eriksson, Per; Fisher, Rachel M. (Corresponding Author).

In: Lipids in Health and Disease, Vol. 11, 115, 2012.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue

AU - Kolak, Maria

AU - Gertow, Joanna

AU - Westerbacka, Jukka

AU - Summers, Scott A.

AU - Liska, Jan

AU - Franco-Cereceda, Anders

AU - Orešič, Matej

AU - Yki-Järvinen, Hannele

AU - Eriksson, Per

AU - Fisher, Rachel M.

PY - 2012

Y1 - 2012

N2 - Background:Inflammation and increased ceramide concentrations characterise adipose tissue of obese women with high liver fat content compared to equally obese women with normal liver fat content. The present study characterises enzymes involved in ceramide metabolism in subcutaneous and intra-abdominal adipose tissue.Methods:Pathways leading to increased ceramide concentrations in inflamed versus non-inflamed adipose tissue were investigated by quantifying expression levels of key enzymes involved in ceramide metabolism. Sphingomyelinases (sphingomyelin phosphodiesterases SMPD1-3) were investigated further using immunohistochemistry to establish their location within adipose tissue, and their mRNA expression levels were determined in subcutaneous and intra-abdominal adipose tissue from both non-obese and obese subject.Results:Gene expression levels of sphingomyelinases, enzymes that hydrolyse sphingomyelin to ceramide, rather than enzymes involved in de novo ceramide synthesis, were higher in inflamed compared to non-inflamed adipose tissue of obese women (with high and normal liver fat contents respectively). Sphingomyelinases were localised to both macrophages and adipocytes, but also to blood vessels and to extracellular regions surrounding vessels within adipose tissue. Expression levels of SMPD3 mRNA correlated significantly with concentrations of different ceramides and sphingomyelins. In both non-obese and obese subjects SMPD3 mRNA levels were higher in the more inflamed intra-abdominal compared to the subcutaneous adipose tissue depot.Conclusions:Generation of ceramides within adipose tissue as a result of sphingomyelinase action may contribute to inflammation in human adipose tissue.

AB - Background:Inflammation and increased ceramide concentrations characterise adipose tissue of obese women with high liver fat content compared to equally obese women with normal liver fat content. The present study characterises enzymes involved in ceramide metabolism in subcutaneous and intra-abdominal adipose tissue.Methods:Pathways leading to increased ceramide concentrations in inflamed versus non-inflamed adipose tissue were investigated by quantifying expression levels of key enzymes involved in ceramide metabolism. Sphingomyelinases (sphingomyelin phosphodiesterases SMPD1-3) were investigated further using immunohistochemistry to establish their location within adipose tissue, and their mRNA expression levels were determined in subcutaneous and intra-abdominal adipose tissue from both non-obese and obese subject.Results:Gene expression levels of sphingomyelinases, enzymes that hydrolyse sphingomyelin to ceramide, rather than enzymes involved in de novo ceramide synthesis, were higher in inflamed compared to non-inflamed adipose tissue of obese women (with high and normal liver fat contents respectively). Sphingomyelinases were localised to both macrophages and adipocytes, but also to blood vessels and to extracellular regions surrounding vessels within adipose tissue. Expression levels of SMPD3 mRNA correlated significantly with concentrations of different ceramides and sphingomyelins. In both non-obese and obese subjects SMPD3 mRNA levels were higher in the more inflamed intra-abdominal compared to the subcutaneous adipose tissue depot.Conclusions:Generation of ceramides within adipose tissue as a result of sphingomyelinase action may contribute to inflammation in human adipose tissue.

U2 - 10.1186/1476-511X-11-115

DO - 10.1186/1476-511X-11-115

M3 - Article

VL - 11

JO - Lipids in Health and Disease

JF - Lipids in Health and Disease

SN - 1476-511X

M1 - 115

ER -