Expression of heterologous Vitreoscilla hemoglobin in barley

Annika Wilhelmson, P.T. Kallio, Kirsi-Marja Oksman-Caldentey, Anna-Maria Nuutila

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Anaerobiosis occurs in seeds of many higher plants during imbibition. After successful germination followed by penetration of the enclosing structures by the radicle, the embryo switches from fermentative to aerobic energy metabolism. The microbial population, which is naturally present on the covering layers of barley grains, may lead to oxygen deficiency by competing for oxygen with the grain tissues. Although imbibed barley grains can survive several days under anoxia, they require oxygen to complete germination. Therefore, novel methods are useful to improve oxygen transfer and enhance germination of seeds. The advances in genetic engineering have opened new possibilities to study oxygen limitation and other critical mechanisms of germination. Previously, heterologous expression of Vitreoscilla hemoglobin (VHb) has been shown to improve either growth properties or productivity of various microbes and plants. Therefore, our metabolic engineering approach was to express the Vitreoscilla hemoglobin gene (vhb) in germinating barley to alleviate adverse effects of inadequate oxygen availability. However, our results show that VHb expression did not improve germination or growth of barley.
Original languageEnglish
Title of host publicationProceedings of the 9th International Barley Genetics Symposium
EditorsJ. Spunar, J. Janikova
Pages394-398
Publication statusPublished - 2004
MoE publication typeNot Eligible
Event9th International Barley Genetics Symposium - Brno, Czech Republic
Duration: 20 Jun 200426 Jun 2004
Conference number: 9

Conference

Conference9th International Barley Genetics Symposium
CountryCzech Republic
CityBrno
Period20/06/0426/06/04

Fingerprint

Vitreoscilla
hemoglobin
barley
oxygen
germination
hypoxia
root radicle
metabolic engineering
anaerobiosis
imbibition
genetic engineering
embryo (plant)
energy metabolism
seed germination
adverse effects
microorganisms
seeds

Keywords

  • barley
  • germination
  • anaerobiosis
  • vitreoscilla
  • hemoglobin
  • transgenic plant

Cite this

Wilhelmson, A., Kallio, P. T., Oksman-Caldentey, K-M., & Nuutila, A-M. (2004). Expression of heterologous Vitreoscilla hemoglobin in barley. In J. Spunar, & J. Janikova (Eds.), Proceedings of the 9th International Barley Genetics Symposium (pp. 394-398)
Wilhelmson, Annika ; Kallio, P.T. ; Oksman-Caldentey, Kirsi-Marja ; Nuutila, Anna-Maria. / Expression of heterologous Vitreoscilla hemoglobin in barley. Proceedings of the 9th International Barley Genetics Symposium. editor / J. Spunar ; J. Janikova. 2004. pp. 394-398
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title = "Expression of heterologous Vitreoscilla hemoglobin in barley",
abstract = "Anaerobiosis occurs in seeds of many higher plants during imbibition. After successful germination followed by penetration of the enclosing structures by the radicle, the embryo switches from fermentative to aerobic energy metabolism. The microbial population, which is naturally present on the covering layers of barley grains, may lead to oxygen deficiency by competing for oxygen with the grain tissues. Although imbibed barley grains can survive several days under anoxia, they require oxygen to complete germination. Therefore, novel methods are useful to improve oxygen transfer and enhance germination of seeds. The advances in genetic engineering have opened new possibilities to study oxygen limitation and other critical mechanisms of germination. Previously, heterologous expression of Vitreoscilla hemoglobin (VHb) has been shown to improve either growth properties or productivity of various microbes and plants. Therefore, our metabolic engineering approach was to express the Vitreoscilla hemoglobin gene (vhb) in germinating barley to alleviate adverse effects of inadequate oxygen availability. However, our results show that VHb expression did not improve germination or growth of barley.",
keywords = "barley, germination, anaerobiosis, vitreoscilla, hemoglobin, transgenic plant",
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Wilhelmson, A, Kallio, PT, Oksman-Caldentey, K-M & Nuutila, A-M 2004, Expression of heterologous Vitreoscilla hemoglobin in barley. in J Spunar & J Janikova (eds), Proceedings of the 9th International Barley Genetics Symposium. pp. 394-398, 9th International Barley Genetics Symposium, Brno, Czech Republic, 20/06/04.

Expression of heterologous Vitreoscilla hemoglobin in barley. / Wilhelmson, Annika; Kallio, P.T.; Oksman-Caldentey, Kirsi-Marja; Nuutila, Anna-Maria.

Proceedings of the 9th International Barley Genetics Symposium. ed. / J. Spunar; J. Janikova. 2004. p. 394-398.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

TY - GEN

T1 - Expression of heterologous Vitreoscilla hemoglobin in barley

AU - Wilhelmson, Annika

AU - Kallio, P.T.

AU - Oksman-Caldentey, Kirsi-Marja

AU - Nuutila, Anna-Maria

PY - 2004

Y1 - 2004

N2 - Anaerobiosis occurs in seeds of many higher plants during imbibition. After successful germination followed by penetration of the enclosing structures by the radicle, the embryo switches from fermentative to aerobic energy metabolism. The microbial population, which is naturally present on the covering layers of barley grains, may lead to oxygen deficiency by competing for oxygen with the grain tissues. Although imbibed barley grains can survive several days under anoxia, they require oxygen to complete germination. Therefore, novel methods are useful to improve oxygen transfer and enhance germination of seeds. The advances in genetic engineering have opened new possibilities to study oxygen limitation and other critical mechanisms of germination. Previously, heterologous expression of Vitreoscilla hemoglobin (VHb) has been shown to improve either growth properties or productivity of various microbes and plants. Therefore, our metabolic engineering approach was to express the Vitreoscilla hemoglobin gene (vhb) in germinating barley to alleviate adverse effects of inadequate oxygen availability. However, our results show that VHb expression did not improve germination or growth of barley.

AB - Anaerobiosis occurs in seeds of many higher plants during imbibition. After successful germination followed by penetration of the enclosing structures by the radicle, the embryo switches from fermentative to aerobic energy metabolism. The microbial population, which is naturally present on the covering layers of barley grains, may lead to oxygen deficiency by competing for oxygen with the grain tissues. Although imbibed barley grains can survive several days under anoxia, they require oxygen to complete germination. Therefore, novel methods are useful to improve oxygen transfer and enhance germination of seeds. The advances in genetic engineering have opened new possibilities to study oxygen limitation and other critical mechanisms of germination. Previously, heterologous expression of Vitreoscilla hemoglobin (VHb) has been shown to improve either growth properties or productivity of various microbes and plants. Therefore, our metabolic engineering approach was to express the Vitreoscilla hemoglobin gene (vhb) in germinating barley to alleviate adverse effects of inadequate oxygen availability. However, our results show that VHb expression did not improve germination or growth of barley.

KW - barley

KW - germination

KW - anaerobiosis

KW - vitreoscilla

KW - hemoglobin

KW - transgenic plant

M3 - Conference article in proceedings

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BT - Proceedings of the 9th International Barley Genetics Symposium

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Wilhelmson A, Kallio PT, Oksman-Caldentey K-M, Nuutila A-M. Expression of heterologous Vitreoscilla hemoglobin in barley. In Spunar J, Janikova J, editors, Proceedings of the 9th International Barley Genetics Symposium. 2004. p. 394-398