Expression of Vitrioscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures

Annika Wilhelmson, Pauli T. Kallio, Kirsi-Marja Oksman-Caldentey, Anna-Maria Nuutila

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

The Vitreoscilla hemoglobin gene (vhb) was introduced into Hyoscyamus muticus with the aim of investigating its effect on growth and alkaloid production of Agrobacterium rhizogenes-induced hairy root cultures. We were able to generate several VHb-expressing hairy root lines with different integration patterns. Substantial somaclonal variation was observed in growth and hyoscyamine production amongst both VHb-expressing lines and controls. Despite this variation, the growth properties of single lines remained stable over time. Expression of VHb was found to improve growth of H. muticus hairy roots in shake-flask cultures. The dry weights of the root cultures expressing Vitreoscilla hemoglobin were on average 18 % higher than those of the controls. VHb expression also increased the volumetric hyoscyamine production, mainly due to the improved growth properties. However, this difference was not statistically significant due to the wide somaclonal variation and fluctuations over time in both VHb and control hairy root lines.
Original languageEnglish
Pages (from-to)48 - 53
Number of pages6
JournalPlanta Medica
Volume71
Issue number1
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

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Hyoscyamus
Cell culture
Hemoglobins
Hyoscyamine
Growth
Agrobacterium
Batch Cell Culture Techniques
Alkaloids
Genes
Weights and Measures

Keywords

  • Hyoscyamus muticus
  • Solanaceae
  • Vitreoscilla hemoglobin
  • hairy root cultures
  • growth improvement

Cite this

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title = "Expression of Vitrioscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures",
abstract = "The Vitreoscilla hemoglobin gene (vhb) was introduced into Hyoscyamus muticus with the aim of investigating its effect on growth and alkaloid production of Agrobacterium rhizogenes-induced hairy root cultures. We were able to generate several VHb-expressing hairy root lines with different integration patterns. Substantial somaclonal variation was observed in growth and hyoscyamine production amongst both VHb-expressing lines and controls. Despite this variation, the growth properties of single lines remained stable over time. Expression of VHb was found to improve growth of H. muticus hairy roots in shake-flask cultures. The dry weights of the root cultures expressing Vitreoscilla hemoglobin were on average 18 {\%} higher than those of the controls. VHb expression also increased the volumetric hyoscyamine production, mainly due to the improved growth properties. However, this difference was not statistically significant due to the wide somaclonal variation and fluctuations over time in both VHb and control hairy root lines.",
keywords = "Hyoscyamus muticus, Solanaceae, Vitreoscilla hemoglobin, hairy root cultures, growth improvement",
author = "Annika Wilhelmson and Kallio, {Pauli T.} and Kirsi-Marja Oksman-Caldentey and Anna-Maria Nuutila",
year = "2005",
doi = "10.1055/s-2005-837750",
language = "English",
volume = "71",
pages = "48 -- 53",
journal = "Planta Medica",
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publisher = "Georg Thieme Verlag",
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}

Expression of Vitrioscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. / Wilhelmson, Annika; Kallio, Pauli T.; Oksman-Caldentey, Kirsi-Marja; Nuutila, Anna-Maria.

In: Planta Medica, Vol. 71, No. 1, 2005, p. 48 - 53.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Expression of Vitrioscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures

AU - Wilhelmson, Annika

AU - Kallio, Pauli T.

AU - Oksman-Caldentey, Kirsi-Marja

AU - Nuutila, Anna-Maria

PY - 2005

Y1 - 2005

N2 - The Vitreoscilla hemoglobin gene (vhb) was introduced into Hyoscyamus muticus with the aim of investigating its effect on growth and alkaloid production of Agrobacterium rhizogenes-induced hairy root cultures. We were able to generate several VHb-expressing hairy root lines with different integration patterns. Substantial somaclonal variation was observed in growth and hyoscyamine production amongst both VHb-expressing lines and controls. Despite this variation, the growth properties of single lines remained stable over time. Expression of VHb was found to improve growth of H. muticus hairy roots in shake-flask cultures. The dry weights of the root cultures expressing Vitreoscilla hemoglobin were on average 18 % higher than those of the controls. VHb expression also increased the volumetric hyoscyamine production, mainly due to the improved growth properties. However, this difference was not statistically significant due to the wide somaclonal variation and fluctuations over time in both VHb and control hairy root lines.

AB - The Vitreoscilla hemoglobin gene (vhb) was introduced into Hyoscyamus muticus with the aim of investigating its effect on growth and alkaloid production of Agrobacterium rhizogenes-induced hairy root cultures. We were able to generate several VHb-expressing hairy root lines with different integration patterns. Substantial somaclonal variation was observed in growth and hyoscyamine production amongst both VHb-expressing lines and controls. Despite this variation, the growth properties of single lines remained stable over time. Expression of VHb was found to improve growth of H. muticus hairy roots in shake-flask cultures. The dry weights of the root cultures expressing Vitreoscilla hemoglobin were on average 18 % higher than those of the controls. VHb expression also increased the volumetric hyoscyamine production, mainly due to the improved growth properties. However, this difference was not statistically significant due to the wide somaclonal variation and fluctuations over time in both VHb and control hairy root lines.

KW - Hyoscyamus muticus

KW - Solanaceae

KW - Vitreoscilla hemoglobin

KW - hairy root cultures

KW - growth improvement

U2 - 10.1055/s-2005-837750

DO - 10.1055/s-2005-837750

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SP - 48

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JO - Planta Medica

JF - Planta Medica

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ER -