Expression of plant proteins in Pichia pastoris

Jaana Toikkanen, Eija Rintala, Michael Bailey, Marja-Leena Niku-Paavola, Teemu Teeri, Richard Fagerström

Research output: Contribution to conferenceConference articleScientific

Abstract

Various plant proteins are to be expressed in Pichia pastoris in our ongoing project. The Invitrogen¡¦s expression vectors and strains are applied on the heterologous production and we have converted the expression vectors Gateway compatible in order to facilitate the cloning steps. The proteins are produced in secreted form either with their native signal sequences or with the aid of mating factor ƒÑ prepro sequence. With one the proteins, the codon usage has been optimized for expression in P. pastoris, and it will be expressed both in secreted form, with its own signal sequence and with MfƒÑ prepro sequence, and intracellularly. Expression of one of these plant proteins will be presented as a case, and the effect of the cultivation conditions on the amount and activity of the product will be discussed.
Original languageEnglish
Publication statusPublished - 2004
MoE publication typeNot Eligible
Event3rd Recombinant Protein Production Meeting: A comparative view on host physiology - Tavira, Portugal
Duration: 11 Nov 200414 Nov 2004

Conference

Conference3rd Recombinant Protein Production Meeting
CountryPortugal
CityTavira
Period11/11/0414/11/04

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    Toikkanen, J., Rintala, E., Bailey, M., Niku-Paavola, M-L., Teeri, T., & Fagerström, R. (2004). Expression of plant proteins in Pichia pastoris. Paper presented at 3rd Recombinant Protein Production Meeting, Tavira, Portugal.