Abstract
Various plant proteins were expressed in Pichia pastoris in our project.
The Invitrogen¡¦s expression vectors and strains were applied to the
heterologous production and we converted the expression vectors Gateway
compatible in order to facilitate the cloning steps. The proteins were
produced in secreted form either with their native signal sequences or with
the aid of mating factor Ą prepro sequence. With one the proteins, the codon
usage had been optimized for expression in P. pastoris, and it was expressed
both in secreted form, with its own signal sequence and with MfĄ prepro
sequence, and intracellularly. Expression of one of these plant proteins will
be presented as a case, and the effect of the cultivation conditions on the
yield and quality of the product will be discussed.
Original language | English |
---|---|
Publication status | Published - 2005 |
MoE publication type | Not Eligible |
Event | Joint 3rd Swiss–Czech and BioTech2005 Symposium "Advances in Yeast Biotechnology" - Wadenswil, Czech Republic Duration: 17 May 2005 → 19 May 2005 |
Conference
Conference | Joint 3rd Swiss–Czech and BioTech2005 Symposium "Advances in Yeast Biotechnology" |
---|---|
Country/Territory | Czech Republic |
City | Wadenswil |
Period | 17/05/05 → 19/05/05 |