Expression of recombinant fungal proteins in Pichia Pastoris

Naoki Sunagawa; Kiyohiko Igarashi

doi:10.1016/B978-0-12-819990-9.00020-2

Expression of recombinant fungal proteins in Pichia Pastoris

Naoki Sunagawa, Kiyohiko Igarashi

VTT Technical Research Centre of Finland

University of Tokyo

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

Abstract

Production of recombinant proteins, including enzymes, in a heterologous host is often necessary to obtain sufficient amounts for functional studies. In particular, yeast expression systems are cost-effective and convenient for producing recombinant proteins having post-translational modifications characteristic of eukaryotes. The yeast Pichia pastoris is a unicellular eukaryote that can be handled in the same environment as Escherichia coli. Here, we present an overview of the construction of expression systems in P. pastoris and their application for the production of recombinant proteins.

Original language	English
Title of host publication	Encyclopedia of Mycology
Editors	Óscar Zaragoza, Arturo Casadevall
Publisher	Elsevier
Pages	518-527
Volume	2
ISBN (Electronic)	978-0-323-85180-0
ISBN (Print)	978-0-1281-9990-9
DOIs	https://doi.org/10.1016/B978-0-12-819990-9.00020-2
Publication status	Published - 1 Jun 2021
MoE publication type	A3 Part of a book or another research book

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10.1016/B978-0-12-819990-9.00020-2

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ER -

Expression of recombinant fungal proteins in Pichia Pastoris

Abstract

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