Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae

Merja Penttilä, Lars Andre, Markku Saloheimo, Päivi Lehtovaara, Jonathan Knowles

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The cDNA copies of the two endo‐β‐1,4‐glucanase genes, egl1 and egl3, from the filamentous fungus Trichoderma reesei were expressed in yeast Saccharomyces cerevisiae under the control of the yeast phosphoglycerate kinase gene promoter. Active EGI and EGIII enzyme was produced and secreted by yeast into the growth medium. The recombinant EGI enzyme was larger and more heterogeneous in size than the native enzyme secreted by Trichoderma, due to differences in the extent of N‐glycosylation between these two organisms. The morphology of the yeast cells producing EGI or EGIII was clearly different from control strain.
Original languageEnglish
Pages (from-to)175-185
JournalYeast
Volume3
Issue number3
DOIs
Publication statusPublished - 1987
MoE publication typeA1 Journal article-refereed

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Trichoderma
Cellulase
Yeast
Saccharomyces cerevisiae
Yeasts
Enzymes
Phosphoglycerate Kinase
Genes
Strain control
Fungi
Complementary DNA
Cells
Growth

Cite this

Penttilä, Merja ; Andre, Lars ; Saloheimo, Markku ; Lehtovaara, Päivi ; Knowles, Jonathan. / Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae. In: Yeast. 1987 ; Vol. 3, No. 3. pp. 175-185.
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Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae. / Penttilä, Merja; Andre, Lars; Saloheimo, Markku; Lehtovaara, Päivi; Knowles, Jonathan.

In: Yeast, Vol. 3, No. 3, 1987, p. 175-185.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae

AU - Penttilä, Merja

AU - Andre, Lars

AU - Saloheimo, Markku

AU - Lehtovaara, Päivi

AU - Knowles, Jonathan

PY - 1987

Y1 - 1987

N2 - The cDNA copies of the two endo‐β‐1,4‐glucanase genes, egl1 and egl3, from the filamentous fungus Trichoderma reesei were expressed in yeast Saccharomyces cerevisiae under the control of the yeast phosphoglycerate kinase gene promoter. Active EGI and EGIII enzyme was produced and secreted by yeast into the growth medium. The recombinant EGI enzyme was larger and more heterogeneous in size than the native enzyme secreted by Trichoderma, due to differences in the extent of N‐glycosylation between these two organisms. The morphology of the yeast cells producing EGI or EGIII was clearly different from control strain.

AB - The cDNA copies of the two endo‐β‐1,4‐glucanase genes, egl1 and egl3, from the filamentous fungus Trichoderma reesei were expressed in yeast Saccharomyces cerevisiae under the control of the yeast phosphoglycerate kinase gene promoter. Active EGI and EGIII enzyme was produced and secreted by yeast into the growth medium. The recombinant EGI enzyme was larger and more heterogeneous in size than the native enzyme secreted by Trichoderma, due to differences in the extent of N‐glycosylation between these two organisms. The morphology of the yeast cells producing EGI or EGIII was clearly different from control strain.

U2 - 10.1002/yea.320030305 |

DO - 10.1002/yea.320030305 |

M3 - Article

VL - 3

SP - 175

EP - 185

JO - Yeast

JF - Yeast

SN - 0749-503X

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ER -