Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources

Emilio Margolles-Clark, Marja Ilmén, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

127 Citations (Scopus)

Abstract

The filamentous fungus Trichoderma reesei produces a multitude of cellulolytic and hemicellulolytic enzymes. The expression of ten hemicellulase-encoding genes was studied by Northern analyses in various culture conditions including polymeric and di- and monomeric sugars. The study included genes encoding two β-xylanases (xyn 1 and xyn2) and a β-mannanase (man 1) which hydrolyze the main chain of hemicellulose polymers by endo fashion, and the acetyl xylan esterase (axe 1), the α-glucuronidase (glr 1), the α-L-arabinofuranosidase (abf1), and three α-galactosidases (agl1, agl2 and agl3), which attack the side groups of hemicellulosic substrates. The genes encoding the oligosaccharide-hydrolysing enzymes β-glucosidase (bgl1) and β-xylosidase (bxl1) were also studied and as a reference the cellulase encoding gene cbh1. A general picture of their relative expression patterns and relative expression levels in particular conditions was obtained. Growth in the presence of cellulose, sophorose, xylobiose, xylan and L-arabitol gave rise to abundant expression of most genes studied. αa-galactosidase I and II, β-xylosidase and α-L-arabinofuranosidase encoding genes were the ones most frequently expressed in the conditions studied, whereas expression of the mannanase and α-galactosidase III was rarely seen. Expression of the genes was not detected in the presence of glucose in the strain QM9414 but derepressed expression of most of them occurred after glucose depletion. Expression of at least xyn1, bxl1, agl1, agl2, agl3 and axe 1 is under control of the glucose repressor cre 1 as studied in the cre1-1 mutant Rut-C30 and Rut-C30 transformed with the wild type cre1 allele.

Original languageEnglish
Pages (from-to)167-179
Number of pages13
JournalJournal of Biotechnology
Volume57
Issue number1-3
DOIs
Publication statusPublished - 16 Sep 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Gene encoding
Fungi
Galactosidases
Carbon
Genes
Xylosidases
Glucose
acetylxylan esterase
Enzymes
Glucosidases
Gene Expression
Xylans
Oligosaccharides
Cellulase
Glucuronidase
Cellulose
Sugars
Polymers
Alleles

Keywords

  • Carbon source regulation
  • Glycosyl hydrolase
  • Induction

Cite this

@article{f57b3bf426274800963ae32a01411ae3,
title = "Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources",
abstract = "The filamentous fungus Trichoderma reesei produces a multitude of cellulolytic and hemicellulolytic enzymes. The expression of ten hemicellulase-encoding genes was studied by Northern analyses in various culture conditions including polymeric and di- and monomeric sugars. The study included genes encoding two β-xylanases (xyn 1 and xyn2) and a β-mannanase (man 1) which hydrolyze the main chain of hemicellulose polymers by endo fashion, and the acetyl xylan esterase (axe 1), the α-glucuronidase (glr 1), the α-L-arabinofuranosidase (abf1), and three α-galactosidases (agl1, agl2 and agl3), which attack the side groups of hemicellulosic substrates. The genes encoding the oligosaccharide-hydrolysing enzymes β-glucosidase (bgl1) and β-xylosidase (bxl1) were also studied and as a reference the cellulase encoding gene cbh1. A general picture of their relative expression patterns and relative expression levels in particular conditions was obtained. Growth in the presence of cellulose, sophorose, xylobiose, xylan and L-arabitol gave rise to abundant expression of most genes studied. αa-galactosidase I and II, β-xylosidase and α-L-arabinofuranosidase encoding genes were the ones most frequently expressed in the conditions studied, whereas expression of the mannanase and α-galactosidase III was rarely seen. Expression of the genes was not detected in the presence of glucose in the strain QM9414 but derepressed expression of most of them occurred after glucose depletion. Expression of at least xyn1, bxl1, agl1, agl2, agl3 and axe 1 is under control of the glucose repressor cre 1 as studied in the cre1-1 mutant Rut-C30 and Rut-C30 transformed with the wild type cre1 allele.",
keywords = "Carbon source regulation, Glycosyl hydrolase, Induction",
author = "Emilio Margolles-Clark and Marja Ilm{\'e}n and Merja Penttil{\"a}",
year = "1997",
month = "9",
day = "16",
doi = "10.1016/S0168-1656(97)00097-7",
language = "English",
volume = "57",
pages = "167--179",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "1-3",

}

Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources. / Margolles-Clark, Emilio; Ilmén, Marja; Penttilä, Merja.

In: Journal of Biotechnology, Vol. 57, No. 1-3, 16.09.1997, p. 167-179.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources

AU - Margolles-Clark, Emilio

AU - Ilmén, Marja

AU - Penttilä, Merja

PY - 1997/9/16

Y1 - 1997/9/16

N2 - The filamentous fungus Trichoderma reesei produces a multitude of cellulolytic and hemicellulolytic enzymes. The expression of ten hemicellulase-encoding genes was studied by Northern analyses in various culture conditions including polymeric and di- and monomeric sugars. The study included genes encoding two β-xylanases (xyn 1 and xyn2) and a β-mannanase (man 1) which hydrolyze the main chain of hemicellulose polymers by endo fashion, and the acetyl xylan esterase (axe 1), the α-glucuronidase (glr 1), the α-L-arabinofuranosidase (abf1), and three α-galactosidases (agl1, agl2 and agl3), which attack the side groups of hemicellulosic substrates. The genes encoding the oligosaccharide-hydrolysing enzymes β-glucosidase (bgl1) and β-xylosidase (bxl1) were also studied and as a reference the cellulase encoding gene cbh1. A general picture of their relative expression patterns and relative expression levels in particular conditions was obtained. Growth in the presence of cellulose, sophorose, xylobiose, xylan and L-arabitol gave rise to abundant expression of most genes studied. αa-galactosidase I and II, β-xylosidase and α-L-arabinofuranosidase encoding genes were the ones most frequently expressed in the conditions studied, whereas expression of the mannanase and α-galactosidase III was rarely seen. Expression of the genes was not detected in the presence of glucose in the strain QM9414 but derepressed expression of most of them occurred after glucose depletion. Expression of at least xyn1, bxl1, agl1, agl2, agl3 and axe 1 is under control of the glucose repressor cre 1 as studied in the cre1-1 mutant Rut-C30 and Rut-C30 transformed with the wild type cre1 allele.

AB - The filamentous fungus Trichoderma reesei produces a multitude of cellulolytic and hemicellulolytic enzymes. The expression of ten hemicellulase-encoding genes was studied by Northern analyses in various culture conditions including polymeric and di- and monomeric sugars. The study included genes encoding two β-xylanases (xyn 1 and xyn2) and a β-mannanase (man 1) which hydrolyze the main chain of hemicellulose polymers by endo fashion, and the acetyl xylan esterase (axe 1), the α-glucuronidase (glr 1), the α-L-arabinofuranosidase (abf1), and three α-galactosidases (agl1, agl2 and agl3), which attack the side groups of hemicellulosic substrates. The genes encoding the oligosaccharide-hydrolysing enzymes β-glucosidase (bgl1) and β-xylosidase (bxl1) were also studied and as a reference the cellulase encoding gene cbh1. A general picture of their relative expression patterns and relative expression levels in particular conditions was obtained. Growth in the presence of cellulose, sophorose, xylobiose, xylan and L-arabitol gave rise to abundant expression of most genes studied. αa-galactosidase I and II, β-xylosidase and α-L-arabinofuranosidase encoding genes were the ones most frequently expressed in the conditions studied, whereas expression of the mannanase and α-galactosidase III was rarely seen. Expression of the genes was not detected in the presence of glucose in the strain QM9414 but derepressed expression of most of them occurred after glucose depletion. Expression of at least xyn1, bxl1, agl1, agl2, agl3 and axe 1 is under control of the glucose repressor cre 1 as studied in the cre1-1 mutant Rut-C30 and Rut-C30 transformed with the wild type cre1 allele.

KW - Carbon source regulation

KW - Glycosyl hydrolase

KW - Induction

UR - http://www.scopus.com/inward/record.url?scp=0343581290&partnerID=8YFLogxK

U2 - 10.1016/S0168-1656(97)00097-7

DO - 10.1016/S0168-1656(97)00097-7

M3 - Article

AN - SCOPUS:0343581290

VL - 57

SP - 167

EP - 179

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1-3

ER -