Expression system for eukaryotic organisms

Dominik Mojzita; Anssi Rantasalo; Jussi Jäntti; Christopher Landowski; Joosu Kuivanen

Expression system for eukaryotic organisms

Dominik Mojzita (Inventor), Anssi Rantasalo (Inventor), Jussi Jäntti (Inventor), Christopher Landowski (Inventor), Joosu Kuivanen (Inventor)

Research output: Patent › Patent

Abstract

The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.

Patent family as of 10.12.2021
AU2017224865 AA 20180913 AU20170224865 20170221
BR112018017088 A2 20190102 BR20181117088 20170221
CA3015440 AA 20180822 CA20173015440 20170221
CN109477115 A 20190315 CN201780020813 20170221
DE602017035634 D1 20210415 DE201760035634T 20170221
DK3405580 T3 20210525 DK20170712530T 20170221
EP3405580 A1 20181128 EP20170712530 20170221
EP3405580 B1 20210331 EP20170712530 20170221
ES2870962 T3 20211028 ES20170712530T 20170221
FI127283 B 20180315 FI20160005137 20160222
FI20165137 A 20170823 FI20160005137 20160222
HU054884 T2 20211028 HU20170712530T 20170221
IN201847035224 A 20180928 IN201847035224 20180919
JP2019505230 T2 20190228 JP20180544246T 20170221
JP6953667 B2 20211027 JP20180544246T 20170221
KR20180117147 A 20181026 KR20187027693 20170221
LT3405580 T 20210810 LT20170712530T 20170221
MX2018010055 A1 20181109 MX20180010055 20170221
PT3405580 T 20210521 PT20170712530T 20170221
US2018371468 AA 20181227 US20170737696 20170221
WO17144777 A1 20170831 WO2017FI50114 20170221

Link to current patent family on right

Original language	English
Patent number	EP3405580
IPC	C12N 15/ 79 A I
Priority date	21/02/17
Publication status	Published - 28 Nov 2018
MoE publication type	H1 Granted patent

Cite this

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title = "Expression system for eukaryotic organisms",

abstract = "The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.Patent family as of 10.12.2021AU2017224865 AA 20180913 AU20170224865 20170221 BR112018017088 A2 20190102 BR20181117088 20170221 CA3015440 AA 20180822 CA20173015440 20170221 CN109477115 A 20190315 CN201780020813 20170221 DE602017035634 D1 20210415 DE201760035634T 20170221 DK3405580 T3 20210525 DK20170712530T 20170221 EP3405580 A1 20181128 EP20170712530 20170221 EP3405580 B1 20210331 EP20170712530 20170221 ES2870962 T3 20211028 ES20170712530T 20170221 FI127283 B 20180315 FI20160005137 20160222 FI20165137 A 20170823 FI20160005137 20160222 HU054884 T2 20211028 HU20170712530T 20170221 IN201847035224 A 20180928 IN201847035224 20180919 JP2019505230 T2 20190228 JP20180544246T 20170221 JP6953667 B2 20211027 JP20180544246T 20170221 KR20180117147 A 20181026 KR20187027693 20170221 LT3405580 T 20210810 LT20170712530T 20170221 MX2018010055 A1 20181109 MX20180010055 20170221 PT3405580 T 20210521 PT20170712530T 20170221 US2018371468 AA 20181227 US20170737696 20170221 WO17144777 A1 20170831 WO2017FI50114 20170221Link to current patent family on right ",

author = "Dominik Mojzita and Anssi Rantasalo and Jussi J{\"a}ntti and Christopher Landowski and Joosu Kuivanen",

year = "2018",

month = nov,

day = "28",

language = "English",

type = "Patent",

note = "EP3405580; C12N 15/ 79 A I",

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TY - PAT

T1 - Expression system for eukaryotic organisms

AU - Mojzita, Dominik

AU - Rantasalo, Anssi

AU - Jäntti, Jussi

AU - Landowski, Christopher

AU - Kuivanen, Joosu

PY - 2018/11/28

Y1 - 2018/11/28

N2 - The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.Patent family as of 10.12.2021AU2017224865 AA 20180913 AU20170224865 20170221 BR112018017088 A2 20190102 BR20181117088 20170221 CA3015440 AA 20180822 CA20173015440 20170221 CN109477115 A 20190315 CN201780020813 20170221 DE602017035634 D1 20210415 DE201760035634T 20170221 DK3405580 T3 20210525 DK20170712530T 20170221 EP3405580 A1 20181128 EP20170712530 20170221 EP3405580 B1 20210331 EP20170712530 20170221 ES2870962 T3 20211028 ES20170712530T 20170221 FI127283 B 20180315 FI20160005137 20160222 FI20165137 A 20170823 FI20160005137 20160222 HU054884 T2 20211028 HU20170712530T 20170221 IN201847035224 A 20180928 IN201847035224 20180919 JP2019505230 T2 20190228 JP20180544246T 20170221 JP6953667 B2 20211027 JP20180544246T 20170221 KR20180117147 A 20181026 KR20187027693 20170221 LT3405580 T 20210810 LT20170712530T 20170221 MX2018010055 A1 20181109 MX20180010055 20170221 PT3405580 T 20210521 PT20170712530T 20170221 US2018371468 AA 20181227 US20170737696 20170221 WO17144777 A1 20170831 WO2017FI50114 20170221Link to current patent family on right

AB - The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.Patent family as of 10.12.2021AU2017224865 AA 20180913 AU20170224865 20170221 BR112018017088 A2 20190102 BR20181117088 20170221 CA3015440 AA 20180822 CA20173015440 20170221 CN109477115 A 20190315 CN201780020813 20170221 DE602017035634 D1 20210415 DE201760035634T 20170221 DK3405580 T3 20210525 DK20170712530T 20170221 EP3405580 A1 20181128 EP20170712530 20170221 EP3405580 B1 20210331 EP20170712530 20170221 ES2870962 T3 20211028 ES20170712530T 20170221 FI127283 B 20180315 FI20160005137 20160222 FI20165137 A 20170823 FI20160005137 20160222 HU054884 T2 20211028 HU20170712530T 20170221 IN201847035224 A 20180928 IN201847035224 20180919 JP2019505230 T2 20190228 JP20180544246T 20170221 JP6953667 B2 20211027 JP20180544246T 20170221 KR20180117147 A 20181026 KR20187027693 20170221 LT3405580 T 20210810 LT20170712530T 20170221 MX2018010055 A1 20181109 MX20180010055 20170221 PT3405580 T 20210521 PT20170712530T 20170221 US2018371468 AA 20181227 US20170737696 20170221 WO17144777 A1 20170831 WO2017FI50114 20170221Link to current patent family on right

UR - https://worldwide.espacenet.com/patent/search/family/058387847/publication/EP3405580A1?q=EP3405580

M3 - Patent

M1 - EP3405580

ER -

Expression system for eukaryotic organisms

Abstract

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