Extraction and determination of enzymes produced by Ceriporiopsis subvermispora during biopulping of Pinus taeda wood chips

Priscila Brasil de Souza-Cruz, Juanita Freer, Matti Siika-aho, Andre Ferraz (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

90 Citations (Scopus)

Abstract

Ceriporiopsis subvermispora is a white-rot basidiomycete that degrades lignin selectively and often is used in biopulping. Development of appropriate experimental procedures for recovery and determination of enzymes produced by C. subvermispora during biopulping is necessary to evaluate the role of each enzyme in this process. In this work, enzymes produced during biopulping were extracted and determined by using a series of substrates. Manganese-dependent peroxidases (MnP) predominated over laccases, which were of minor significance. The lack of laccases was further confirmed by assaying several substrates such as syrindaldazine, o-dianisidine and ABTS. A basal oxidation of syringaldazine and phenol red in the absence of exogenous Mn2+ led to the assumption that the extracts contained some Mn-independent peroxidase or enough Mn2+ to initiate MnP catalyzed reactions. The presence of Mn2+ was evidenced since phenol red was not oxidized by a 16-h-dialysed extract. Addition of 111 μM of Mn2+ to the reaction medium restored the oxidative activity. Lignin peroxidase activity was not detected in the extracts based on the Azure B assay. Cellulase activities were very low while xylanases predominated. The xylanolytic complex was deficient in beta-xylosidase. Successive extractions were performed for a quantitative recovery of the enzymes adsorbed on wood chips. The first 4-h extraction recovered an average of 50% of the total enzymatic activities.

Original languageEnglish
Pages (from-to)228 - 234
Number of pages7
JournalEnzyme and Microbial Technology
Volume34
Issue number3-4
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Keywords

  • Ceriporiopsis subvermispora
  • Oxidative enzymes
  • Biopulping
  • White-rot fungi

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