Extrusion cooking in enzymatic liquefaction of wheat starch

Pekka Reinikainen, Tapani Suortti, Juhani Olkku, Yrjö Mälkki, Pekka Linko

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)

Abstract

Wheat starch was treated for gelatinization, partial liquefaction, and subsequent further hydrolysis by extrusion cooking together with a thermostable Bacillus licheniformis α‐amylase followed by batch incubation of the extrudates at 80°C. The degree of hydrolysis depended on the extrusion feed moisture content, enzyme concentration, mass temperature during extrusion cooking, and the length of the counter screw elements. The degree of hydrolysis was followed both by dextrose equivalent and by molecular weight distribution pattern as determined by high performance gel permeation chromatography. A reaction velocity model was shown to fit well to the kinetic data obtained. The enzyme was only incompletely inactivated by mercuric chloride suggesting the presence of also other than sulfhydryl dependent amylase.

Original languageEnglish
Pages (from-to)20 - 26
Number of pages7
JournalStärke
Volume38
Issue number1
DOIs
Publication statusPublished - 1986
MoE publication typeNot Eligible

Fingerprint

wheat starch
Cooking
Liquefaction
extrusion
Starch
Triticum
Extrusion
cooking
Hydrolysis
hydrolysis
Amylases
amylases
mercuric chloride
Mercuric Chloride
Bacillus licheniformis
Gel permeation chromatography
Bacilli
Enzymes
Molecular weight distribution
gelatinization

Cite this

Reinikainen, P., Suortti, T., Olkku, J., Mälkki, Y., & Linko, P. (1986). Extrusion cooking in enzymatic liquefaction of wheat starch. Stärke, 38(1), 20 - 26. https://doi.org/10.1002/star.19860380106
Reinikainen, Pekka ; Suortti, Tapani ; Olkku, Juhani ; Mälkki, Yrjö ; Linko, Pekka. / Extrusion cooking in enzymatic liquefaction of wheat starch. In: Stärke. 1986 ; Vol. 38, No. 1. pp. 20 - 26.
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Reinikainen, P, Suortti, T, Olkku, J, Mälkki, Y & Linko, P 1986, 'Extrusion cooking in enzymatic liquefaction of wheat starch', Stärke, vol. 38, no. 1, pp. 20 - 26. https://doi.org/10.1002/star.19860380106

Extrusion cooking in enzymatic liquefaction of wheat starch. / Reinikainen, Pekka; Suortti, Tapani; Olkku, Juhani; Mälkki, Yrjö; Linko, Pekka.

In: Stärke, Vol. 38, No. 1, 1986, p. 20 - 26.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Reinikainen, Pekka

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AU - Olkku, Juhani

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AU - Linko, Pekka

PY - 1986

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AB - Wheat starch was treated for gelatinization, partial liquefaction, and subsequent further hydrolysis by extrusion cooking together with a thermostable Bacillus licheniformis α‐amylase followed by batch incubation of the extrudates at 80°C. The degree of hydrolysis depended on the extrusion feed moisture content, enzyme concentration, mass temperature during extrusion cooking, and the length of the counter screw elements. The degree of hydrolysis was followed both by dextrose equivalent and by molecular weight distribution pattern as determined by high performance gel permeation chromatography. A reaction velocity model was shown to fit well to the kinetic data obtained. The enzyme was only incompletely inactivated by mercuric chloride suggesting the presence of also other than sulfhydryl dependent amylase.

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Reinikainen P, Suortti T, Olkku J, Mälkki Y, Linko P. Extrusion cooking in enzymatic liquefaction of wheat starch. Stärke. 1986;38(1):20 - 26. https://doi.org/10.1002/star.19860380106