F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets

J. J. Joensuu (Corresponding Author), F. Verdonck, A. Ehrström, M. Peltola, H. Siljander-Rasi, Anna-Maria Nuutila, Kirsi-Marja Oksman-Caldentey, Teemu Teeri, E. Cox, B. M. Goddeeris, V. Niklander-Teeri

Research output: Contribution to journalArticleScientificpeer-review

29 Citations (Scopus)

Abstract

Transgenic plants are attractive bioreactors to large-scale production of recombinant proteins because of their relatively low cost. This study reports for the first time the use of transgenic plants to reduce enterotoxigenic Escherichia coli (ETEC) excretion in its natural host species. The DNA sequence encoding the major subunit and adhesin FaeG of F4+ ETEC was transformed into edible alfalfa plants. Targeting of FaeG production to chloroplasts led to FaeG levels of up to 1% of the total soluble protein fraction of the transgenic alfalfa. Recombinant plant-produced FaeG (pFaeG) remained stable for 2 years when the plant material was dried and stored at room temperature. Intragastric immunization of piglets with pFaeG induced a weak F4-specific humoral response. Co-administration of pFaeG and the mucosal adjuvant cholera toxin (CT) enhanced the immune response against FaeG, reflected a better induction of an F4-specific immune response. In addition, the intragastric co-administration of CT with pFaeG significantly reduced F4+ E. coli excretion following F4+ ETEC challenge as compared with pigs that had received nontransgenic plant material. In conclusion, transgenic plants producing the FaeG subunit protein could be used for production and delivery of oral vaccines against F4+ ETEC infections.
Original languageEnglish
Pages (from-to)2387-2394
Number of pages8
JournalVaccine
Volume24
Issue number13
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Bacterial Adhesins
Enterotoxigenic Escherichia coli
enterotoxigenic Escherichia coli
adhesins
Medicago sativa
piglets
alfalfa
Genetically Modified Plants
excretion
transgenic plants
cholera toxin
Cholera Toxin
immune response
Escherichia coli Infections
Edible Plants
Protein Subunits
Bioreactors
protein subunits
Chloroplasts
bioreactors

Keywords

  • F4 (K88) fimbriae
  • Enterotoxigenic Escherichia coli
  • Plant-made vaccine
  • Alfalfa
  • Chloroplast targeting
  • Piglet

Cite this

Joensuu, J. J., Verdonck, F., Ehrström, A., Peltola, M., Siljander-Rasi, H., Nuutila, A-M., ... Niklander-Teeri, V. (2006). F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets. Vaccine, 24(13), 2387-2394. https://doi.org/10.1016/j.vaccine.2005.11.056
Joensuu, J. J. ; Verdonck, F. ; Ehrström, A. ; Peltola, M. ; Siljander-Rasi, H. ; Nuutila, Anna-Maria ; Oksman-Caldentey, Kirsi-Marja ; Teeri, Teemu ; Cox, E. ; Goddeeris, B. M. ; Niklander-Teeri, V. / F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets. In: Vaccine. 2006 ; Vol. 24, No. 13. pp. 2387-2394.
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title = "F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets",
abstract = "Transgenic plants are attractive bioreactors to large-scale production of recombinant proteins because of their relatively low cost. This study reports for the first time the use of transgenic plants to reduce enterotoxigenic Escherichia coli (ETEC) excretion in its natural host species. The DNA sequence encoding the major subunit and adhesin FaeG of F4+ ETEC was transformed into edible alfalfa plants. Targeting of FaeG production to chloroplasts led to FaeG levels of up to 1{\%} of the total soluble protein fraction of the transgenic alfalfa. Recombinant plant-produced FaeG (pFaeG) remained stable for 2 years when the plant material was dried and stored at room temperature. Intragastric immunization of piglets with pFaeG induced a weak F4-specific humoral response. Co-administration of pFaeG and the mucosal adjuvant cholera toxin (CT) enhanced the immune response against FaeG, reflected a better induction of an F4-specific immune response. In addition, the intragastric co-administration of CT with pFaeG significantly reduced F4+ E. coli excretion following F4+ ETEC challenge as compared with pigs that had received nontransgenic plant material. In conclusion, transgenic plants producing the FaeG subunit protein could be used for production and delivery of oral vaccines against F4+ ETEC infections.",
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author = "Joensuu, {J. J.} and F. Verdonck and A. Ehrstr{\"o}m and M. Peltola and H. Siljander-Rasi and Anna-Maria Nuutila and Kirsi-Marja Oksman-Caldentey and Teemu Teeri and E. Cox and Goddeeris, {B. M.} and V. Niklander-Teeri",
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Joensuu, JJ, Verdonck, F, Ehrström, A, Peltola, M, Siljander-Rasi, H, Nuutila, A-M, Oksman-Caldentey, K-M, Teeri, T, Cox, E, Goddeeris, BM & Niklander-Teeri, V 2006, 'F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets', Vaccine, vol. 24, no. 13, pp. 2387-2394. https://doi.org/10.1016/j.vaccine.2005.11.056

F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets. / Joensuu, J. J. (Corresponding Author); Verdonck, F.; Ehrström, A.; Peltola, M.; Siljander-Rasi, H.; Nuutila, Anna-Maria; Oksman-Caldentey, Kirsi-Marja; Teeri, Teemu; Cox, E.; Goddeeris, B. M.; Niklander-Teeri, V.

In: Vaccine, Vol. 24, No. 13, 2006, p. 2387-2394.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets

AU - Joensuu, J. J.

AU - Verdonck, F.

AU - Ehrström, A.

AU - Peltola, M.

AU - Siljander-Rasi, H.

AU - Nuutila, Anna-Maria

AU - Oksman-Caldentey, Kirsi-Marja

AU - Teeri, Teemu

AU - Cox, E.

AU - Goddeeris, B. M.

AU - Niklander-Teeri, V.

PY - 2006

Y1 - 2006

N2 - Transgenic plants are attractive bioreactors to large-scale production of recombinant proteins because of their relatively low cost. This study reports for the first time the use of transgenic plants to reduce enterotoxigenic Escherichia coli (ETEC) excretion in its natural host species. The DNA sequence encoding the major subunit and adhesin FaeG of F4+ ETEC was transformed into edible alfalfa plants. Targeting of FaeG production to chloroplasts led to FaeG levels of up to 1% of the total soluble protein fraction of the transgenic alfalfa. Recombinant plant-produced FaeG (pFaeG) remained stable for 2 years when the plant material was dried and stored at room temperature. Intragastric immunization of piglets with pFaeG induced a weak F4-specific humoral response. Co-administration of pFaeG and the mucosal adjuvant cholera toxin (CT) enhanced the immune response against FaeG, reflected a better induction of an F4-specific immune response. In addition, the intragastric co-administration of CT with pFaeG significantly reduced F4+ E. coli excretion following F4+ ETEC challenge as compared with pigs that had received nontransgenic plant material. In conclusion, transgenic plants producing the FaeG subunit protein could be used for production and delivery of oral vaccines against F4+ ETEC infections.

AB - Transgenic plants are attractive bioreactors to large-scale production of recombinant proteins because of their relatively low cost. This study reports for the first time the use of transgenic plants to reduce enterotoxigenic Escherichia coli (ETEC) excretion in its natural host species. The DNA sequence encoding the major subunit and adhesin FaeG of F4+ ETEC was transformed into edible alfalfa plants. Targeting of FaeG production to chloroplasts led to FaeG levels of up to 1% of the total soluble protein fraction of the transgenic alfalfa. Recombinant plant-produced FaeG (pFaeG) remained stable for 2 years when the plant material was dried and stored at room temperature. Intragastric immunization of piglets with pFaeG induced a weak F4-specific humoral response. Co-administration of pFaeG and the mucosal adjuvant cholera toxin (CT) enhanced the immune response against FaeG, reflected a better induction of an F4-specific immune response. In addition, the intragastric co-administration of CT with pFaeG significantly reduced F4+ E. coli excretion following F4+ ETEC challenge as compared with pigs that had received nontransgenic plant material. In conclusion, transgenic plants producing the FaeG subunit protein could be used for production and delivery of oral vaccines against F4+ ETEC infections.

KW - F4 (K88) fimbriae

KW - Enterotoxigenic Escherichia coli

KW - Plant-made vaccine

KW - Alfalfa

KW - Chloroplast targeting

KW - Piglet

U2 - 10.1016/j.vaccine.2005.11.056

DO - 10.1016/j.vaccine.2005.11.056

M3 - Article

VL - 24

SP - 2387

EP - 2394

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 13

ER -