Factors affecting the activation of pulps with laccase

Anna Suurnäkki (Corresponding Author), Tarja Oksanen, M. Orlandi, L. Zoia, C. Canevali, L. Viikari

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Activation of fibres by radical formation is the first step when aiming at oxidative coupling of new functional groups on the fibre bound lignin. In this work, factors affecting the amount of phenoxy radicals created to unbleached TMP, CTMP, softwood kraft and hardwood kraft pulp fibres in the laccase catalysed oxidation were determined by EPR. Laccase was able to catalyse the oxidation of all the pulps studied. The reactivity of the pulp was found to be affected by both the physical accessibility of lignin in the fibres and the chemistry of the surface lignin accessible to laccase. Laccase dosage, use of extra oxygen in the laccase catalysed radicalization reaction, treatment time and also the amount and type of low-molecular weight compounds (LMWC) present in the pulp were all found to contribute to the radical content of pulp fibres measured after the enzymatic reaction. It could not been excluded that two types of reactions take place during the radical formation in fibres. Within the fibre matrix there may be both fibre material bound and soluble lignin fragments differing with respect to accessibility, molecular weight or chemical structure which can be radicalized at various rates, and the formed radicals may also undergo cross-coupling reactions reducing the amount of the total radicals.
Original languageEnglish
Pages (from-to)153-158
JournalEnzyme and Microbial Technology
Volume46
Issue number3-4
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignin
Pulp
Chemical activation
Fibers
Molecular Weight
Oxidative Coupling
Thymidine Monophosphate
Cross Reactions
Molecular weight
Oxidation
Kraft pulp
Softwoods
Hardwoods
Oxygen
Functional groups
Paramagnetic resonance

Keywords

  • Activation of fibres
  • Reactivity of pulps
  • Laccase treatment
  • Phenoxy radicals
  • EPR

Cite this

Suurnäkki, A., Oksanen, T., Orlandi, M., Zoia, L., Canevali, C., & Viikari, L. (2010). Factors affecting the activation of pulps with laccase. Enzyme and Microbial Technology, 46(3-4), 153-158. https://doi.org/10.1016/j.enzmictec.2009.11.009
Suurnäkki, Anna ; Oksanen, Tarja ; Orlandi, M. ; Zoia, L. ; Canevali, C. ; Viikari, L. / Factors affecting the activation of pulps with laccase. In: Enzyme and Microbial Technology. 2010 ; Vol. 46, No. 3-4. pp. 153-158.
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Suurnäkki, A, Oksanen, T, Orlandi, M, Zoia, L, Canevali, C & Viikari, L 2010, 'Factors affecting the activation of pulps with laccase', Enzyme and Microbial Technology, vol. 46, no. 3-4, pp. 153-158. https://doi.org/10.1016/j.enzmictec.2009.11.009

Factors affecting the activation of pulps with laccase. / Suurnäkki, Anna (Corresponding Author); Oksanen, Tarja; Orlandi, M.; Zoia, L.; Canevali, C.; Viikari, L.

In: Enzyme and Microbial Technology, Vol. 46, No. 3-4, 2010, p. 153-158.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Factors affecting the activation of pulps with laccase

AU - Suurnäkki, Anna

AU - Oksanen, Tarja

AU - Orlandi, M.

AU - Zoia, L.

AU - Canevali, C.

AU - Viikari, L.

PY - 2010

Y1 - 2010

N2 - Activation of fibres by radical formation is the first step when aiming at oxidative coupling of new functional groups on the fibre bound lignin. In this work, factors affecting the amount of phenoxy radicals created to unbleached TMP, CTMP, softwood kraft and hardwood kraft pulp fibres in the laccase catalysed oxidation were determined by EPR. Laccase was able to catalyse the oxidation of all the pulps studied. The reactivity of the pulp was found to be affected by both the physical accessibility of lignin in the fibres and the chemistry of the surface lignin accessible to laccase. Laccase dosage, use of extra oxygen in the laccase catalysed radicalization reaction, treatment time and also the amount and type of low-molecular weight compounds (LMWC) present in the pulp were all found to contribute to the radical content of pulp fibres measured after the enzymatic reaction. It could not been excluded that two types of reactions take place during the radical formation in fibres. Within the fibre matrix there may be both fibre material bound and soluble lignin fragments differing with respect to accessibility, molecular weight or chemical structure which can be radicalized at various rates, and the formed radicals may also undergo cross-coupling reactions reducing the amount of the total radicals.

AB - Activation of fibres by radical formation is the first step when aiming at oxidative coupling of new functional groups on the fibre bound lignin. In this work, factors affecting the amount of phenoxy radicals created to unbleached TMP, CTMP, softwood kraft and hardwood kraft pulp fibres in the laccase catalysed oxidation were determined by EPR. Laccase was able to catalyse the oxidation of all the pulps studied. The reactivity of the pulp was found to be affected by both the physical accessibility of lignin in the fibres and the chemistry of the surface lignin accessible to laccase. Laccase dosage, use of extra oxygen in the laccase catalysed radicalization reaction, treatment time and also the amount and type of low-molecular weight compounds (LMWC) present in the pulp were all found to contribute to the radical content of pulp fibres measured after the enzymatic reaction. It could not been excluded that two types of reactions take place during the radical formation in fibres. Within the fibre matrix there may be both fibre material bound and soluble lignin fragments differing with respect to accessibility, molecular weight or chemical structure which can be radicalized at various rates, and the formed radicals may also undergo cross-coupling reactions reducing the amount of the total radicals.

KW - Activation of fibres

KW - Reactivity of pulps

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KW - Phenoxy radicals

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JF - Enzyme and Microbial Technology

SN - 0141-0229

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ER -

Suurnäkki A, Oksanen T, Orlandi M, Zoia L, Canevali C, Viikari L. Factors affecting the activation of pulps with laccase. Enzyme and Microbial Technology. 2010;46(3-4):153-158. https://doi.org/10.1016/j.enzmictec.2009.11.009