Factors affecting the conversion of apple polyphenols to phenolic acids and fruit matrix to short-chain fatty acids by human faecal microbiota in vitro

Sarah Bazzocco, Ismo Mattila, Sylvain Guyot, Catherine M. G. C. Renard, Anna-Marja Aura

Research output: Contribution to journalArticleScientificpeer-review

67 Citations (Scopus)

Abstract

Proanthocyanidins (PAs) in apples are condensed tannins comprised mostly of (−)-epicatechin units with some terminal (+)-catechins. PAs, especially those having a long chain-length, are absorbed in the upper intestine only to a small extent and are passed to the colon. In the colon they are subjected to microbial metabolism by colonic microbiota. In the present article, the ability of human microbiota to ferment apple PAs is studied. Freeze-dried fruit preparations (apple, enzymatically digested apple, isolated cell-walls, isolated PAs or ciders) from two varieties, Marie Ménard and Avrolles, containing PAs of different chain lengths, were compared. Fermentation studies were performed in an in vitro colon model using human faecal microbiota as an inoculum. The maximal extent of conversion to known microbial metabolites, was observed at late time point for Marie Ménard cider, having short PAs. In this case, the initial dose also contributed to the extent of conversion. Long-chain PAs were able to inhibit the in vitro microbial metabolism of PAs shown as low maxima at early time points. Presence of isolated PAs also suppressed SCFA formation from carbohydrates as compared with that from apple cell wall or faecal suspension without substrates. The low maximal extents at early time points suggest that there is a competition between the inhibitory effect of the PAs on microbial activity, and the ability to convert PAs by the microbiota.
Original languageEnglish
Pages (from-to)442 - 452
JournalEuropean Journal of Nutrition
Volume47
Issue number8
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Keywords

  • cell-wall
  • gut microbiota
  • in vitro fermentation
  • Malus domestica Borkh
  • procyanidin

Fingerprint Dive into the research topics of 'Factors affecting the conversion of apple polyphenols to phenolic acids and fruit matrix to short-chain fatty acids by human faecal microbiota in vitro'. Together they form a unique fingerprint.

  • Cite this