Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli

A. Usvalampi (Corresponding Author), K. Kiviharju, M. Leisola, Antti Nyyssölä

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Factors affecting the production of the rare sugar l-xylulose from xylitol using resting cells were investigated. An E. coli BPT228 strain that recombinantly expresses a gene for xylitol dehydrogenase was used in the experiments. The ratio of xylitol to l-xylulose was three times lower in the cytoplasm than in the medium. The effects of pH, temperature, shaking speed, and initial xylitol concentration on l-xylulose production were investigated in shaking flasks using statistical experimental design methods. The highest production rates were found at high shaking speed and at high temperature (over 44°C). The optimal pH for both productivity and conversion was between 7.5 and 8.0, and the optimal xylitol concentration was in the range 250–350 g l−1. A specific productivity of 1.09 ± 0.10 g g−1 h−1 was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy.
Original languageEnglish
Pages (from-to)1323-1330
Number of pages8
JournalJournal of industrial microbiology and biotechnology
Volume36
Issue number10
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Xylulose
Xylitol
Escherichia coli
Bioreactors
Productivity
D-Xylulose Reductase
Sugars
Design of experiments
Temperature
Genes
Experiments
Cytoplasm
Research Design

Keywords

  • L-Xylulose
  • rare sugars
  • resting cells
  • xylitol
  • xylitol dehydrogenase

Cite this

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title = "Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli",
abstract = "Factors affecting the production of the rare sugar l-xylulose from xylitol using resting cells were investigated. An E. coli BPT228 strain that recombinantly expresses a gene for xylitol dehydrogenase was used in the experiments. The ratio of xylitol to l-xylulose was three times lower in the cytoplasm than in the medium. The effects of pH, temperature, shaking speed, and initial xylitol concentration on l-xylulose production were investigated in shaking flasks using statistical experimental design methods. The highest production rates were found at high shaking speed and at high temperature (over 44°C). The optimal pH for both productivity and conversion was between 7.5 and 8.0, and the optimal xylitol concentration was in the range 250–350 g l−1. A specific productivity of 1.09 ± 0.10 g g−1 h−1 was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy.",
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Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli. / Usvalampi, A. (Corresponding Author); Kiviharju, K.; Leisola, M.; Nyyssölä, Antti.

In: Journal of industrial microbiology and biotechnology, Vol. 36, No. 10, 2009, p. 1323-1330.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli

AU - Usvalampi, A.

AU - Kiviharju, K.

AU - Leisola, M.

AU - Nyyssölä, Antti

PY - 2009

Y1 - 2009

N2 - Factors affecting the production of the rare sugar l-xylulose from xylitol using resting cells were investigated. An E. coli BPT228 strain that recombinantly expresses a gene for xylitol dehydrogenase was used in the experiments. The ratio of xylitol to l-xylulose was three times lower in the cytoplasm than in the medium. The effects of pH, temperature, shaking speed, and initial xylitol concentration on l-xylulose production were investigated in shaking flasks using statistical experimental design methods. The highest production rates were found at high shaking speed and at high temperature (over 44°C). The optimal pH for both productivity and conversion was between 7.5 and 8.0, and the optimal xylitol concentration was in the range 250–350 g l−1. A specific productivity of 1.09 ± 0.10 g g−1 h−1 was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy.

AB - Factors affecting the production of the rare sugar l-xylulose from xylitol using resting cells were investigated. An E. coli BPT228 strain that recombinantly expresses a gene for xylitol dehydrogenase was used in the experiments. The ratio of xylitol to l-xylulose was three times lower in the cytoplasm than in the medium. The effects of pH, temperature, shaking speed, and initial xylitol concentration on l-xylulose production were investigated in shaking flasks using statistical experimental design methods. The highest production rates were found at high shaking speed and at high temperature (over 44°C). The optimal pH for both productivity and conversion was between 7.5 and 8.0, and the optimal xylitol concentration was in the range 250–350 g l−1. A specific productivity of 1.09 ± 0.10 g g−1 h−1 was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy.

KW - L-Xylulose

KW - rare sugars

KW - resting cells

KW - xylitol

KW - xylitol dehydrogenase

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DO - 10.1007/s10295-009-0616-x

M3 - Article

VL - 36

SP - 1323

EP - 1330

JO - Journal of industrial microbiology and biotechnology

JF - Journal of industrial microbiology and biotechnology

SN - 1367-5435

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ER -