Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli

A. Usvalampi (Corresponding Author), K. Kiviharju, M. Leisola, Antti Nyyssölä

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Factors affecting the production of the rare sugar l-xylulose from xylitol using resting cells were investigated. An E. coli BPT228 strain that recombinantly expresses a gene for xylitol dehydrogenase was used in the experiments. The ratio of xylitol to l-xylulose was three times lower in the cytoplasm than in the medium. The effects of pH, temperature, shaking speed, and initial xylitol concentration on l-xylulose production were investigated in shaking flasks using statistical experimental design methods. The highest production rates were found at high shaking speed and at high temperature (over 44°C). The optimal pH for both productivity and conversion was between 7.5 and 8.0, and the optimal xylitol concentration was in the range 250–350 g l−1. A specific productivity of 1.09 ± 0.10 g g−1 h−1 was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy.
Original languageEnglish
Pages (from-to)1323-1330
Number of pages8
JournalJournal of industrial microbiology and biotechnology
Volume36
Issue number10
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Keywords

  • L-Xylulose
  • rare sugars
  • resting cells
  • xylitol
  • xylitol dehydrogenase

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