Factors affecting the regeneration capacity of isolated barley microspores (Hordeum vulgare L.)

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30 Citations (Scopus)

Abstract

The culture of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite malting barley cultivar) was studied. A careful choice of culture steps resulted in an average regeneration frequency of 300 green plants per starting material spike. Strong seasonal variation in regeneration capacity was observed. The choice of a cold pretreatment method affected the viability of microspores. A cold pretreatment of the collected starting material at +4°C for 4 weeks was needed for the efficient regeneration of green plants from isolated microspore cultures. Glutamine omission from and copper additions to microspore culture were studied. The omission of glutamine did not affect the number of regenerated green plants but did result in an increase in the number of regenerated albino plants. The addition of copper did not improve the regeneration capacity of isolated barley microspores. Transformation by particle bombardment of isolated microspores did not result in the production of transgenic plants.
Original languageEnglish
Pages (from-to)403-407
Number of pages5
JournalPlant Cell Reports
Volume20
Issue number5
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

microspores
Hordeum vulgare
barley
glutamine
pretreatment
copper
malting barley
albino
transgenic plants
inflorescences
seasonal variation
viability
cultivars

Keywords

  • Barley
  • Hordeum vulgare L.
  • Microspores
  • Regeneration capacity
  • Seasonal variation

Cite this

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title = "Factors affecting the regeneration capacity of isolated barley microspores (Hordeum vulgare L.)",
abstract = "The culture of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite malting barley cultivar) was studied. A careful choice of culture steps resulted in an average regeneration frequency of 300 green plants per starting material spike. Strong seasonal variation in regeneration capacity was observed. The choice of a cold pretreatment method affected the viability of microspores. A cold pretreatment of the collected starting material at +4°C for 4 weeks was needed for the efficient regeneration of green plants from isolated microspore cultures. Glutamine omission from and copper additions to microspore culture were studied. The omission of glutamine did not affect the number of regenerated green plants but did result in an increase in the number of regenerated albino plants. The addition of copper did not improve the regeneration capacity of isolated barley microspores. Transformation by particle bombardment of isolated microspores did not result in the production of transgenic plants.",
keywords = "Barley, Hordeum vulgare L., Microspores, Regeneration capacity, Seasonal variation",
author = "Anneli Ritala and Leena Mannonen and Kirsi-Marja Oksman-Caldentey",
year = "2001",
doi = "10.1007/s002990100345",
language = "English",
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pages = "403--407",
journal = "Plant Cell Reports",
issn = "0721-7714",
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Factors affecting the regeneration capacity of isolated barley microspores (Hordeum vulgare L.). / Ritala, Anneli; Mannonen, Leena; Oksman-Caldentey, Kirsi-Marja.

In: Plant Cell Reports, Vol. 20, No. 5, 2001, p. 403-407.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Factors affecting the regeneration capacity of isolated barley microspores (Hordeum vulgare L.)

AU - Ritala, Anneli

AU - Mannonen, Leena

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2001

Y1 - 2001

N2 - The culture of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite malting barley cultivar) was studied. A careful choice of culture steps resulted in an average regeneration frequency of 300 green plants per starting material spike. Strong seasonal variation in regeneration capacity was observed. The choice of a cold pretreatment method affected the viability of microspores. A cold pretreatment of the collected starting material at +4°C for 4 weeks was needed for the efficient regeneration of green plants from isolated microspore cultures. Glutamine omission from and copper additions to microspore culture were studied. The omission of glutamine did not affect the number of regenerated green plants but did result in an increase in the number of regenerated albino plants. The addition of copper did not improve the regeneration capacity of isolated barley microspores. Transformation by particle bombardment of isolated microspores did not result in the production of transgenic plants.

AB - The culture of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite malting barley cultivar) was studied. A careful choice of culture steps resulted in an average regeneration frequency of 300 green plants per starting material spike. Strong seasonal variation in regeneration capacity was observed. The choice of a cold pretreatment method affected the viability of microspores. A cold pretreatment of the collected starting material at +4°C for 4 weeks was needed for the efficient regeneration of green plants from isolated microspore cultures. Glutamine omission from and copper additions to microspore culture were studied. The omission of glutamine did not affect the number of regenerated green plants but did result in an increase in the number of regenerated albino plants. The addition of copper did not improve the regeneration capacity of isolated barley microspores. Transformation by particle bombardment of isolated microspores did not result in the production of transgenic plants.

KW - Barley

KW - Hordeum vulgare L.

KW - Microspores

KW - Regeneration capacity

KW - Seasonal variation

U2 - 10.1007/s002990100345

DO - 10.1007/s002990100345

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JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

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