Ferrocene-containing polymers as electron transfer mediators in carbon paste electrodes modified with PQQ-dependent aldose dehydrogenase

Maria Smolander (Corresponding Author), Lo Gorton, Hung Sui Lee, Terje Skotheim, Hsing-Lin Lan

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The aim of this work was to improve an aldose‐detecting biosensor based on quinoprotein aldose dehydrogenase (ALDH). Polymer‐bound ferrocene derivatives with four different structures were studied as electron transfer mediators between the coenzyme of ALDH, pyrroloquinoline qninone (PQQ), and the conducting electrode material.
The ferrocene polymers were mixed into carbon paste, which was used for the preparation of aldose‐detecting electrodes by immobilizing ALDH on the carbon paste surface by adsorption. The current responses of the ferrocene polymer modified electrodes were studied and the effect of the polymer structure on the response was examined.
The electrodes modified with all four polymers showed maximum response at the working potential around 300 mV (vs. Ag/AgCl). The operational stability of the ferrocene polymer modified electrodes was better than that of corresponding dimethylferrocene modified electrodes.
The ferrocene polymer modified electrodes seemed to be suitable for the measurement of real samples.
Original languageEnglish
Pages (from-to)941-946
JournalElectroanalysis
Volume7
Issue number10
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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Ointments
Oxidoreductases
Polymers
Carbon
Electrodes
Electrons
Coenzymes
ferrocene
pyrroloquinoline
Biosensors
Derivatives
Adsorption

Cite this

@article{235829ad9ce741a6aa3e50bcba2c64c6,
title = "Ferrocene-containing polymers as electron transfer mediators in carbon paste electrodes modified with PQQ-dependent aldose dehydrogenase",
abstract = "The aim of this work was to improve an aldose‐detecting biosensor based on quinoprotein aldose dehydrogenase (ALDH). Polymer‐bound ferrocene derivatives with four different structures were studied as electron transfer mediators between the coenzyme of ALDH, pyrroloquinoline qninone (PQQ), and the conducting electrode material. The ferrocene polymers were mixed into carbon paste, which was used for the preparation of aldose‐detecting electrodes by immobilizing ALDH on the carbon paste surface by adsorption. The current responses of the ferrocene polymer modified electrodes were studied and the effect of the polymer structure on the response was examined. The electrodes modified with all four polymers showed maximum response at the working potential around 300 mV (vs. Ag/AgCl). The operational stability of the ferrocene polymer modified electrodes was better than that of corresponding dimethylferrocene modified electrodes. The ferrocene polymer modified electrodes seemed to be suitable for the measurement of real samples.",
author = "Maria Smolander and Lo Gorton and Lee, {Hung Sui} and Terje Skotheim and Hsing-Lin Lan",
note = "Project code: B5SU00064",
year = "1995",
doi = "10.1002/elan.1140071006",
language = "English",
volume = "7",
pages = "941--946",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
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}

Ferrocene-containing polymers as electron transfer mediators in carbon paste electrodes modified with PQQ-dependent aldose dehydrogenase. / Smolander, Maria (Corresponding Author); Gorton, Lo; Lee, Hung Sui; Skotheim, Terje; Lan, Hsing-Lin.

In: Electroanalysis, Vol. 7, No. 10, 1995, p. 941-946.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Ferrocene-containing polymers as electron transfer mediators in carbon paste electrodes modified with PQQ-dependent aldose dehydrogenase

AU - Smolander, Maria

AU - Gorton, Lo

AU - Lee, Hung Sui

AU - Skotheim, Terje

AU - Lan, Hsing-Lin

N1 - Project code: B5SU00064

PY - 1995

Y1 - 1995

N2 - The aim of this work was to improve an aldose‐detecting biosensor based on quinoprotein aldose dehydrogenase (ALDH). Polymer‐bound ferrocene derivatives with four different structures were studied as electron transfer mediators between the coenzyme of ALDH, pyrroloquinoline qninone (PQQ), and the conducting electrode material. The ferrocene polymers were mixed into carbon paste, which was used for the preparation of aldose‐detecting electrodes by immobilizing ALDH on the carbon paste surface by adsorption. The current responses of the ferrocene polymer modified electrodes were studied and the effect of the polymer structure on the response was examined. The electrodes modified with all four polymers showed maximum response at the working potential around 300 mV (vs. Ag/AgCl). The operational stability of the ferrocene polymer modified electrodes was better than that of corresponding dimethylferrocene modified electrodes. The ferrocene polymer modified electrodes seemed to be suitable for the measurement of real samples.

AB - The aim of this work was to improve an aldose‐detecting biosensor based on quinoprotein aldose dehydrogenase (ALDH). Polymer‐bound ferrocene derivatives with four different structures were studied as electron transfer mediators between the coenzyme of ALDH, pyrroloquinoline qninone (PQQ), and the conducting electrode material. The ferrocene polymers were mixed into carbon paste, which was used for the preparation of aldose‐detecting electrodes by immobilizing ALDH on the carbon paste surface by adsorption. The current responses of the ferrocene polymer modified electrodes were studied and the effect of the polymer structure on the response was examined. The electrodes modified with all four polymers showed maximum response at the working potential around 300 mV (vs. Ag/AgCl). The operational stability of the ferrocene polymer modified electrodes was better than that of corresponding dimethylferrocene modified electrodes. The ferrocene polymer modified electrodes seemed to be suitable for the measurement of real samples.

U2 - 10.1002/elan.1140071006

DO - 10.1002/elan.1140071006

M3 - Article

VL - 7

SP - 941

EP - 946

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

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ER -