Fertile transgenic barley by particle bombardment of immature embryos

Anneli Ritala, Kristian Aspegren, Ulrika Kurten, Marjatta Salmenkallio-Marttila, Leena Mannonen, Riitta Hannus, Veli Kauppinen, Teemu Teeri, Tor-Magnus Enari

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Transgenic, fertile barley (Hordeum vulgare L.) from the Finnish elite cultivar Kymppi was obtained by particle bombardment of immature embryos. Immature embryos were bombarded to the embryonic axis side and grown to plants without selection. Neomycin phosphotransferase II (NPTII) activity was screened in small plantlets. One out of a total of 227 plants expressed the transferred nptII gene. This plant has until now produced 98 fertile spikes (T0), and four of the 90 T0 spikes analyzed to date contained the nptII gene. These shoots were further analyzed and they expressed the transferred gene. From green grains, embryos were isolated and grown to plantlets (T1). The four transgenic shoots of Toivo (the T0 plant) produced 25 plantlets as T1 progeny. Altogether fifteen of these T1 plants carried the transferred nptII gene as detected with the PCR technique, fourteen of which expressed the nptII gene. The integration and inheritance of the transferred nptII gene was confirmed by Southern blot hybridization. Although present as several copies, the transferred gene was inherited as a single Mendelian locus into the T2 progeny.
Original languageEnglish
Pages (from-to)317-325
Number of pages9
JournalPlant Molecular Biology
Volume24
Issue number2
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

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Hordeum
embryo (plant)
Embryonic Structures
immatures
barley
genetically modified organisms
Genes
genes
plantlets
inflorescences
Kanamycin Kinase
kanamycin kinase
shoots
Southern Blotting
Southern blotting
Hordeum vulgare
inheritance (genetics)
hybridization
Polymerase Chain Reaction
loci

Cite this

Ritala, A., Aspegren, K., Kurten, U., Salmenkallio-Marttila, M., Mannonen, L., Hannus, R., ... Enari, T-M. (1994). Fertile transgenic barley by particle bombardment of immature embryos. Plant Molecular Biology, 24(2), 317-325. https://doi.org/10.1007/BF00020170
Ritala, Anneli ; Aspegren, Kristian ; Kurten, Ulrika ; Salmenkallio-Marttila, Marjatta ; Mannonen, Leena ; Hannus, Riitta ; Kauppinen, Veli ; Teeri, Teemu ; Enari, Tor-Magnus. / Fertile transgenic barley by particle bombardment of immature embryos. In: Plant Molecular Biology. 1994 ; Vol. 24, No. 2. pp. 317-325.
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abstract = "Transgenic, fertile barley (Hordeum vulgare L.) from the Finnish elite cultivar Kymppi was obtained by particle bombardment of immature embryos. Immature embryos were bombarded to the embryonic axis side and grown to plants without selection. Neomycin phosphotransferase II (NPTII) activity was screened in small plantlets. One out of a total of 227 plants expressed the transferred nptII gene. This plant has until now produced 98 fertile spikes (T0), and four of the 90 T0 spikes analyzed to date contained the nptII gene. These shoots were further analyzed and they expressed the transferred gene. From green grains, embryos were isolated and grown to plantlets (T1). The four transgenic shoots of Toivo (the T0 plant) produced 25 plantlets as T1 progeny. Altogether fifteen of these T1 plants carried the transferred nptII gene as detected with the PCR technique, fourteen of which expressed the nptII gene. The integration and inheritance of the transferred nptII gene was confirmed by Southern blot hybridization. Although present as several copies, the transferred gene was inherited as a single Mendelian locus into the T2 progeny.",
author = "Anneli Ritala and Kristian Aspegren and Ulrika Kurten and Marjatta Salmenkallio-Marttila and Leena Mannonen and Riitta Hannus and Veli Kauppinen and Teemu Teeri and Tor-Magnus Enari",
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Ritala, A, Aspegren, K, Kurten, U, Salmenkallio-Marttila, M, Mannonen, L, Hannus, R, Kauppinen, V, Teeri, T & Enari, T-M 1994, 'Fertile transgenic barley by particle bombardment of immature embryos', Plant Molecular Biology, vol. 24, no. 2, pp. 317-325. https://doi.org/10.1007/BF00020170

Fertile transgenic barley by particle bombardment of immature embryos. / Ritala, Anneli; Aspegren, Kristian; Kurten, Ulrika; Salmenkallio-Marttila, Marjatta; Mannonen, Leena; Hannus, Riitta; Kauppinen, Veli; Teeri, Teemu; Enari, Tor-Magnus.

In: Plant Molecular Biology, Vol. 24, No. 2, 1994, p. 317-325.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Fertile transgenic barley by particle bombardment of immature embryos

AU - Ritala, Anneli

AU - Aspegren, Kristian

AU - Kurten, Ulrika

AU - Salmenkallio-Marttila, Marjatta

AU - Mannonen, Leena

AU - Hannus, Riitta

AU - Kauppinen, Veli

AU - Teeri, Teemu

AU - Enari, Tor-Magnus

N1 - Project code: BIO3030

PY - 1994

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N2 - Transgenic, fertile barley (Hordeum vulgare L.) from the Finnish elite cultivar Kymppi was obtained by particle bombardment of immature embryos. Immature embryos were bombarded to the embryonic axis side and grown to plants without selection. Neomycin phosphotransferase II (NPTII) activity was screened in small plantlets. One out of a total of 227 plants expressed the transferred nptII gene. This plant has until now produced 98 fertile spikes (T0), and four of the 90 T0 spikes analyzed to date contained the nptII gene. These shoots were further analyzed and they expressed the transferred gene. From green grains, embryos were isolated and grown to plantlets (T1). The four transgenic shoots of Toivo (the T0 plant) produced 25 plantlets as T1 progeny. Altogether fifteen of these T1 plants carried the transferred nptII gene as detected with the PCR technique, fourteen of which expressed the nptII gene. The integration and inheritance of the transferred nptII gene was confirmed by Southern blot hybridization. Although present as several copies, the transferred gene was inherited as a single Mendelian locus into the T2 progeny.

AB - Transgenic, fertile barley (Hordeum vulgare L.) from the Finnish elite cultivar Kymppi was obtained by particle bombardment of immature embryos. Immature embryos were bombarded to the embryonic axis side and grown to plants without selection. Neomycin phosphotransferase II (NPTII) activity was screened in small plantlets. One out of a total of 227 plants expressed the transferred nptII gene. This plant has until now produced 98 fertile spikes (T0), and four of the 90 T0 spikes analyzed to date contained the nptII gene. These shoots were further analyzed and they expressed the transferred gene. From green grains, embryos were isolated and grown to plantlets (T1). The four transgenic shoots of Toivo (the T0 plant) produced 25 plantlets as T1 progeny. Altogether fifteen of these T1 plants carried the transferred nptII gene as detected with the PCR technique, fourteen of which expressed the nptII gene. The integration and inheritance of the transferred nptII gene was confirmed by Southern blot hybridization. Although present as several copies, the transferred gene was inherited as a single Mendelian locus into the T2 progeny.

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Ritala A, Aspegren K, Kurten U, Salmenkallio-Marttila M, Mannonen L, Hannus R et al. Fertile transgenic barley by particle bombardment of immature embryos. Plant Molecular Biology. 1994;24(2):317-325. https://doi.org/10.1007/BF00020170