Fibers with different solubility characteristics alter similarly the metabolic activity of intestinal microbiota in rats fed cereal brans and inulin

Soile M. Gråsten (Corresponding Author), Anne-Maria Pajari, Kirsi-Helena Liukkonen, Sirpa Karppinen, Hannu Mykkänen

Research output: Contribution to journalArticleScientificpeer-review

29 Citations (Scopus)

Abstract

We compared the effects of different sources of fiber (rye-bran, oat-bran, wheat-bran) and inulin on the activity of fecal bacterial enzymes and concentration of short chain fatty acids (SCFA) in the colonic contents of rats. A diet without fiber was used as a control. The cereal brans and inulin had similar, increasing effect on the activities of beta-glucuronidase and urease (P < 0.05). All bran diets also increased beta-glucosidase activity while the increase by inulin was non-significant. Concentration of total SCFA was higher in the inulin and rye-bran groups compared to non-fiber and wheat-bran groups (P < 0.05). Concentration of butyrate was higher in rye-bran and inulin groups compared to the non-fiber group (P < 0.05), and that of propionate higher in the inulin group compared to non-fiber and wheat-bran groups (P < 0.05). The SCFA profile in the oat-bran group did not differ significantly from SCFA profile in the other groups. These findings indicate that the source of the fiber does not markedly affect the enzyme activities of the fecal bacterial microbiota, but may cause differences in the profile of SCFA in the rat colon.
Original languageEnglish
Pages (from-to)1435-1444
JournalNutrition Research
Volume22
Issue number12
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Inulin
Volatile Fatty Acids
Solubility
Dietary Fiber
Diet
beta-Glucosidase
Urease
Butyrates
Microbiota
Glucuronidase
Propionates
Enzymes
Gastrointestinal Microbiome
Edible Grain
Colon
Secale

Keywords

  • rye
  • oats
  • wheat
  • inulin
  • bacterial enzymes
  • intestinal fermentation
  • rat

Cite this

Gråsten, Soile M. ; Pajari, Anne-Maria ; Liukkonen, Kirsi-Helena ; Karppinen, Sirpa ; Mykkänen, Hannu. / Fibers with different solubility characteristics alter similarly the metabolic activity of intestinal microbiota in rats fed cereal brans and inulin. In: Nutrition Research. 2002 ; Vol. 22, No. 12. pp. 1435-1444.
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abstract = "We compared the effects of different sources of fiber (rye-bran, oat-bran, wheat-bran) and inulin on the activity of fecal bacterial enzymes and concentration of short chain fatty acids (SCFA) in the colonic contents of rats. A diet without fiber was used as a control. The cereal brans and inulin had similar, increasing effect on the activities of beta-glucuronidase and urease (P < 0.05). All bran diets also increased beta-glucosidase activity while the increase by inulin was non-significant. Concentration of total SCFA was higher in the inulin and rye-bran groups compared to non-fiber and wheat-bran groups (P < 0.05). Concentration of butyrate was higher in rye-bran and inulin groups compared to the non-fiber group (P < 0.05), and that of propionate higher in the inulin group compared to non-fiber and wheat-bran groups (P < 0.05). The SCFA profile in the oat-bran group did not differ significantly from SCFA profile in the other groups. These findings indicate that the source of the fiber does not markedly affect the enzyme activities of the fecal bacterial microbiota, but may cause differences in the profile of SCFA in the rat colon.",
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Fibers with different solubility characteristics alter similarly the metabolic activity of intestinal microbiota in rats fed cereal brans and inulin. / Gråsten, Soile M. (Corresponding Author); Pajari, Anne-Maria; Liukkonen, Kirsi-Helena; Karppinen, Sirpa; Mykkänen, Hannu.

In: Nutrition Research, Vol. 22, No. 12, 2002, p. 1435-1444.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Gråsten, Soile M.

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AB - We compared the effects of different sources of fiber (rye-bran, oat-bran, wheat-bran) and inulin on the activity of fecal bacterial enzymes and concentration of short chain fatty acids (SCFA) in the colonic contents of rats. A diet without fiber was used as a control. The cereal brans and inulin had similar, increasing effect on the activities of beta-glucuronidase and urease (P < 0.05). All bran diets also increased beta-glucosidase activity while the increase by inulin was non-significant. Concentration of total SCFA was higher in the inulin and rye-bran groups compared to non-fiber and wheat-bran groups (P < 0.05). Concentration of butyrate was higher in rye-bran and inulin groups compared to the non-fiber group (P < 0.05), and that of propionate higher in the inulin group compared to non-fiber and wheat-bran groups (P < 0.05). The SCFA profile in the oat-bran group did not differ significantly from SCFA profile in the other groups. These findings indicate that the source of the fiber does not markedly affect the enzyme activities of the fecal bacterial microbiota, but may cause differences in the profile of SCFA in the rat colon.

KW - rye

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KW - intestinal fermentation

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