Fibrolytic enzyme treatment prior to ensiling increased press-juice and crude protein yield from grass silage

Marketta Rinne (Corresponding Author), Erika Winquist, Ville Pihlajaniemi, Piritta Niemi, Arja Seppälä, Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Grass is a versatile raw material for green biorefineries and preserving it as silage provides a year-round feedstock. The objective of the current study was to evaluate the effect of fibrolytic enzyme application on silage as a feedstock for a biorefinery. Two batches of grass (mixture of timothy and meadow fescue) silages were ensiled in pilot scale after fibrolytic enzyme was applied to them at four levels. Enzyme application increased fibre degradation linearly during ensiling and increased lactic and acetic acid concentrations in the silage. Simultaneously, silage fermentation quality improved as indicated by decreasing pH and ammonia values. Press-juice and crude protein yields increased in response to the fibrolytic enzyme application, which is beneficial in a biorefinery concept for retrieving valuable nutrients from grass matrix. Optimized ensiling methodology can be considered as a pretreatment for a biorefinery process.
Original languageEnglish
Article number122572
JournalBioresource Technology
Volume299
Early online date6 Dec 2019
DOIs
Publication statusE-pub ahead of print - 6 Dec 2019
MoE publication typeA1 Journal article-refereed

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silage
Enzymes
grass
enzyme
Proteins
protein
Feedstocks
Lactic acid
Ammonia
Acetic acid
Acetic Acid
Fermentation
Nutrients
Raw materials
acetic acid
fermentation
meadow
Degradation
ammonia
Fibers

Cite this

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title = "Fibrolytic enzyme treatment prior to ensiling increased press-juice and crude protein yield from grass silage",
abstract = "Grass is a versatile raw material for green biorefineries and preserving it as silage provides a year-round feedstock. The objective of the current study was to evaluate the effect of fibrolytic enzyme application on silage as a feedstock for a biorefinery. Two batches of grass (mixture of timothy and meadow fescue) silages were ensiled in pilot scale after fibrolytic enzyme was applied to them at four levels. Enzyme application increased fibre degradation linearly during ensiling and increased lactic and acetic acid concentrations in the silage. Simultaneously, silage fermentation quality improved as indicated by decreasing pH and ammonia values. Press-juice and crude protein yields increased in response to the fibrolytic enzyme application, which is beneficial in a biorefinery concept for retrieving valuable nutrients from grass matrix. Optimized ensiling methodology can be considered as a pretreatment for a biorefinery process.",
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Fibrolytic enzyme treatment prior to ensiling increased press-juice and crude protein yield from grass silage. / Rinne, Marketta (Corresponding Author); Winquist, Erika; Pihlajaniemi, Ville; Niemi, Piritta; Seppälä, Arja; Siika-aho, Matti.

In: Bioresource Technology, Vol. 299, 122572, 03.2020.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Rinne, Marketta

AU - Winquist, Erika

AU - Pihlajaniemi, Ville

AU - Niemi, Piritta

AU - Seppälä, Arja

AU - Siika-aho, Matti

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AB - Grass is a versatile raw material for green biorefineries and preserving it as silage provides a year-round feedstock. The objective of the current study was to evaluate the effect of fibrolytic enzyme application on silage as a feedstock for a biorefinery. Two batches of grass (mixture of timothy and meadow fescue) silages were ensiled in pilot scale after fibrolytic enzyme was applied to them at four levels. Enzyme application increased fibre degradation linearly during ensiling and increased lactic and acetic acid concentrations in the silage. Simultaneously, silage fermentation quality improved as indicated by decreasing pH and ammonia values. Press-juice and crude protein yields increased in response to the fibrolytic enzyme application, which is beneficial in a biorefinery concept for retrieving valuable nutrients from grass matrix. Optimized ensiling methodology can be considered as a pretreatment for a biorefinery process.

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